Abstract
Sterilization procedure, media composition, explants selection and control of physical environment are critical for successful cultures and callus induction with surface sterilization being very challenging in most plants. Five different sterilization methods were evaluated to come up with the best for subsequent use to establish an in vitro regeneration method for the induction of callus in Curcuma caesia using excised leaf and rhizome explants. Murashige and Skoog (MS) media supplemented with various concentration of 2,4-Dichlorophenoxy acetic acid (2,4-D)/Indole-3-acetic acid (IAA) (0.5- 5.0mg/L), singly or in combination with Benzyl aminopurine (BAP)/Kinetin (KIN) (0.1-5.0mg/L), 0.3% sucrose and 0.08% agar were used. The result of the sterilization procedures showed 15% NaHClO3 (5min) + 70% Ethanol (30s) + 0.1% HgCl2 (5min) to be the most effective in controlling contamination in C. caesia among all the treatments tested. The response to callus induction was found to depend on the type of explants used and growth regulators combination. Leaf explants gave the highest percentage of callus induction. Highest percentage of callus induction (66.70%) was obtained in the growth regulator combination of 2, 4-D (0.5mg/L) + BAP (0.1mg/L) and least (14.29%) in IAA (2.0mg/L) + BAP (0.5mg/L). Equal and higher concentration of 2, 4-D + BAP of 5.0mg/L each also provided better result (40.00%). No callus was obtained in all the single concentration of 2, 4-D used.
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Claims 6
Claim · model-written summary
The 15% NaHClO3 + 70% ethanol + 0.1% HgCl2 sterilization sequence was the most effective in controlling contamination in Curcuma caesia
- Taxon
- Curcuma caesia
Curcuma caesiat0 - Treatment
- 15% NaHClO3 (5min) + 70% Ethanol (30s) + 0.1% HgCl2 (5min)e1
- Outcome
- controlling contaminatione1
- Direction
- most effectivee1
- Comparator
- all the treatments testede1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Curcuma caesia”
e1“15% NaHClO3 (5min) + 70% Ethanol (30s) + 0.1% HgCl2 (5min) to be the most effective in controlling contamination in C. caesia among all the treatments tested”
Claim · model-written summary
Leaf explants gave the highest percentage of callus induction in Curcuma caesia
- Taxon
- Curcuma caesiaCurcuma caesiat0
- Organ / stage
- Leafe2
- Treatment
- Leaf explantse2
- Outcome
- percentage of callus inductione2
- Direction
- higheste2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Curcuma caesia”
e2“Leaf explants gave the highest percentage of callus induction”
Claim · model-written summary
Callus induction was highest (66.70%) with 2,4-D 0.5 mg/L plus BAP 0.1 mg/L in Curcuma caesia
- Taxon
- Curcuma caesiaCurcuma caesiat0
- Treatment
- 2, 4-D (0.5mg/L) + BAP (0.1mg/L)e3
- Outcome
- percentage of callus inductione3
- Direction
- Higheste3
- Value
- 66.70%e3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Curcuma caesia”
e3“Highest percentage of callus induction (66.70%) was obtained in the growth regulator combination of 2, 4-D (0.5mg/L) + BAP (0.1mg/L) and least (14.29%) in IAA (2.0mg/L) + BAP (0.5mg/L)”
Claim · model-written summary
Callus induction was lowest (14.29%) with IAA 2.0 mg/L plus BAP 0.5 mg/L in Curcuma caesia
- Taxon
- Curcuma caesiaCurcuma caesiat0
- Treatment
- IAA (2.0mg/L) + BAP (0.5mg/L)e3
- Outcome
- percentage of callus inductione3
- Direction
- leaste3
- Value
- 14.29%e3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Curcuma caesia”
e3“Highest percentage of callus induction (66.70%) was obtained in the growth regulator combination of 2, 4-D (0.5mg/L) + BAP (0.1mg/L) and least (14.29%) in IAA (2.0mg/L) + BAP (0.5mg/L)”
Claim · model-written summary
Equal and higher 2,4-D plus BAP at 5.0 mg/L each gave a better callus result (40.00%)
- Taxon
- Curcuma caesiaCurcuma caesiat0
- Treatment
- 2, 4-D + BAP of 5.0mg/L eache4
- Outcome
- resulte4
- Direction
- bettere4
- Value
- 40.00%e4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Curcuma caesia”
e4“Equal and higher concentration of 2, 4-D + BAP of 5.0mg/L each also provided better result (40.00%)”
Claim · model-written summary
No callus was obtained with any single concentration of 2,4-D in Curcuma caesia
- Taxon
- Curcuma caesiaCurcuma caesiat0
- Treatment
- single concentration of 2, 4-De5
- Outcome
- No callus was obtainede5
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Curcuma caesia”
e5“No callus was obtained in all the single concentration of 2, 4-D used”
The same questions in other papers
2,4-D · growth · Curcuma caesia (black zedoary)Results agree
1 paper in the same direction as this paper
- positive
N-benzyladenine · growth · Curcuma caesia (black zedoary)Results agree
2 papers in the same direction as this paper
- positive
- positive
in vitro · growth · Curcuma caesia (black zedoary)Results agree
2 papers in the same direction as this paper
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.