Abstract
Ethylene is the plant hormone that controls several features of plant growth and development. The rate-limiting step in its synthesis is the formation of the ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC) from S-adenosylmethionine (AdoMet), catalyzed by ACC synthase. We have isolated a complementary DNA sequence encoding ACC synthase from zucchini (Cucurbita) fruits. The biological activity of the clone was confirmed by the ability of the cloned sequence to direct ACC synthase activity in Escherichia coli and yeast. In vivo studies using the ACC cDNA as probe showed that the ACC synthase gene is induced by a diverse group of inducers, including wounding, Li+ ions, and the plant hormone auxin.
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Claims 6
Claim · model-written summary
A cDNA encoding ACC synthase was isolated from zucchini fruits.
CucurbitaNot reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We have isolated a complementary DNA sequence encoding ACC synthase from zucchini (Cucurbita) fruits”
Claim · model-written summary
The cloned sequence directed ACC synthase activity in Escherichia coli.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the cloned sequence to direct ACC synthase activity in Escherichia coli”
t0“zucchini (Cucurbita)”
Claim · model-written summary
The cloned sequence directed ACC synthase activity in yeast.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the cloned sequence to direct ACC synthase activity in Escherichia coli and yeast”
t0“zucchini (Cucurbita)”
Claim · model-written summary
Wounding induced the ACC synthase gene.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ACC synthase gene is induced by a diverse group of inducers, including wounding, Li+ ions, and the plant hormone auxin”
t0“zucchini (Cucurbita)”
Claim · model-written summary
Li+ ions induced the ACC synthase gene.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ACC synthase gene is induced by a diverse group of inducers, including wounding, Li+ ions, and the plant hormone auxin”
t0“zucchini (Cucurbita)”
Claim · model-written summary
Auxin induced the ACC synthase gene.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ACC synthase gene is induced by a diverse group of inducers, including wounding, Li+ ions, and the plant hormone auxin”
t0“zucchini (Cucurbita)”
The same questions in other papers
class · molecular: expression · Cucurbita (pumpkins)Results agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.