Abstract
Nrat1 is a plasma membrane-localized aluminum transporter recently identified in rice, which is a member of Nramp family. Here, we further characterized this transporter in terms of transport substrate specificity. Heterologous assay in yeast showed that Al transport activity by Nrat1 was unaffected by the presence of high concentration of Ca, but significantly inhibited by trivalent ions including Yb and Ga, analogs of Al. Knockout of Nrat1 did not affect the uptake of Cd and Mn in rice. On the other hand, over-expression of Nrat1 led to enhanced Al uptake by rice roots compared with wild-type rice, but did not affect Cd uptake. These results provide further evidence that unlike other Nramp members, Nrat1 is an influx transporter for trivalent Al ion.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 5
Claim · model-written summary
High Ca did not affect Nrat1 aluminum transport in yeast.
Not reportedTaxonComparator
Verbatim
e0“Al transport activity by Nrat1 was unaffected by the presence of high concentration of Ca”
Claim · model-written summary
Trivalent Yb and Ga significantly inhibited Nrat1 aluminum transport in yeast.
- Treatment
- trivalent ions including Yb and Gae0
- Gene
- Nrat1e0
- Outcome
- Al transport activitye0
- Direction
- inhibitede0
Not reportedTaxonComparator
Verbatim
e0“Al transport activity by Nrat1 was unaffected by the presence of high concentration of Ca, but significantly inhibited by trivalent ions including Yb and Ga, analogs of Al”
Claim · model-written summary
Nrat1 knockout did not affect rice uptake of Cd and Mn.
- Taxon
- rice
Oryza sativae0 - Treatment
- Knockout of Nrat1e0
- Gene
- Nrat1e0
- Outcome
- uptake of Cd and Mne0
- Direction
- did not affecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Knockout of Nrat1 did not affect the uptake of Cd and Mn in rice”
Claim · model-written summary
Nrat1 overexpression enhanced aluminum uptake by rice roots relative to wild type.
- Taxon
- riceOryza sativae0
- Organ / stage
- rootse0
- Treatment
- over-expression of Nrat1e0
- Gene
- Nrat1e0
- Outcome
- Al uptakee0
- Direction
- enhancede0
- Comparator
- wild-type ricee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“over-expression of Nrat1 led to enhanced Al uptake by rice roots compared with wild-type rice”
Claim · model-written summary
Nrat1 overexpression did not affect Cd uptake in rice.
- Taxon
- riceOryza sativae0
- Treatment
- over-expression of Nrat1e0
- Gene
- Nrat1e0
- Outcome
- Cd uptakee0
- Direction
- did not affecte0
- Comparator
- wild-type ricee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“over-expression of Nrat1 led to enhanced Al uptake by rice roots compared with wild-type rice, but did not affect Cd uptake”
The same questions in other papers
calcium atom · nutrient: content · Oryza sativa (rice)Positive and negative results
4 papers in the same direction as this paper · 23 in the opposite direction
- positive
- positive
- positive
24 papers more in this group
See all 28 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.