Abstract
A cDNA encoding a putative H+-translocating pyrophosphatase (H+-PPase) has been cloned from Zea mays by suppression subtractive hybridization (SSH) coupled with in silico cloning approach. The isolated 2974 bp full-length cDNA named ZmGPP contains a single 2400 bp open reading frame encoding a putative protein of 799 amino acids. The predicted protein has 16 transmembrane domains and is significantly similar to Golgi apparatus resident type-II H+-PPase from Arabidopsis thaliana. DNA gel blotting analysis shows that ZmGPP is a low-copy gene. Organ expression pattern analysis reveals that ZmGPPexpressed highly in leaf and tassel, followed by in stem, root, and ear. The Real-time RT-PCR assays showed that the expression of ZmGPP was up-regulated both in shoots and roots of maize seedlings under dehydration, cold and high salt stresses. Those results suggest that the ZmGPP product may play an important role in abiotic stress tolerance of Z. mays.
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Claims 10
Claim · model-written summary
ZmGPP cDNA was cloned from maize.
Zea maysOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A cDNA encoding a putative H+-translocating pyrophosphatase (H+-PPase) has been cloned from Zea mays by suppression subtractive hybridization (SSH) coupled with in silico cloning approach.”
e1“The isolated 2974 bp full-length cDNA named ZmGPP”
Claim · model-written summary
ZmGPP has 2400 bp open reading frame.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The isolated 2974 bp full-length cDNA named ZmGPP contains a single 2400 bp open reading frame encoding a putative protein of 799 amino acids.”
t0“Zea mays”
Claim · model-written summary
ZmGPP has 799 amino acids putative protein.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The isolated 2974 bp full-length cDNA named ZmGPP contains a single 2400 bp open reading frame encoding a putative protein of 799 amino acids.”
t0“Zea mays”
Claim · model-written summary
ZmGPP has 16 transmembrane domains.
Not reportedGene
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The predicted protein has 16 transmembrane domains and is significantly similar to Golgi apparatus resident type-II H+-PPase from Arabidopsis thaliana.”
t0“Zea mays”
Claim · model-written summary
The predicted protein is significantly similar to Arabidopsis type-II H+-PPase.
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The predicted protein has 16 transmembrane domains and is significantly similar to Golgi apparatus resident type-II H+-PPase from Arabidopsis thaliana.”
t0“Zea mays”
Claim · model-written summary
ZmGPP is a low-copy gene.
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“DNA gel blotting analysis shows that ZmGPP is a low-copy gene.”
t0“Zea mays”
Claim · model-written summary
ZmGPP expression was highest in leaf and tassel, followed by stem, root, and ear.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Organ expression pattern analysis reveals that ZmGPPexpressed highly in leaf and tassel, followed by in stem, root, and ear.”
t0“Zea mays”
Claim · model-written summary
ZmGPP expression was up-regulated in shoots and roots under dehydration.
- Organ / stage
- shoots and rootse0
- Treatment
- dehydratione0
- Gene
- ZmGPPe0
- Outcome
- expression of ZmGPPe0
- Direction
- up-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The Real-time RT-PCR assays showed that the expression of ZmGPP was up-regulated both in shoots and roots of maize seedlings under dehydration, cold and high salt stresses.”
Claim · model-written summary
ZmGPP expression was up-regulated in shoots and roots under cold.
- Organ / stage
- shoots and rootse0
- Treatment
- colde0
- Gene
- ZmGPPe0
- Outcome
- expression of ZmGPPe0
- Direction
- up-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The Real-time RT-PCR assays showed that the expression of ZmGPP was up-regulated both in shoots and roots of maize seedlings under dehydration, cold and high salt stresses.”
Claim · model-written summary
ZmGPP expression was up-regulated in shoots and roots under high salt stresses.
- Organ / stage
- shoots and rootse0
- Treatment
- high salt stressese0
- Gene
- ZmGPPe0
- Outcome
- expression of ZmGPPe0
- Direction
- up-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The Real-time RT-PCR assays showed that the expression of ZmGPP was up-regulated both in shoots and roots of maize seedlings under dehydration, cold and high salt stresses.”
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See all 65 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.