Abstract
Plasticity of the tobacco genome was studied by testing the DNAs of protoplast-derived regenerants with three different repetitive DNA sequences by the method of quantitative DNA/DNA hybridizations. A large population of 91 regenerants belonging to 35 different protoclones was analysed and a high degree of heterogeneity in the contents of the different DNA repeats was detected. The contents of middle repetitive sequences of two types were more stable or changed in the same direction, while the highly repetitive sequence varied independently and displayed a significant reduction in comparison with the two other sequences. Comparing the variation within the subpopulations of plants of the same clonal origin and the variation among the protoclones led to a conclusion that the pre-existing DNA variability in the starting plant material and/or thein vitro stress during the very early stages of protoclone regeneration played a decisive role in the formation of modified genomes in regenerants.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 5
Claim · model-written summary
Repetitive DNA contents showed high heterogeneity among tobacco regenerants.
- Taxon
- tobacco
Nicotiana tabacumt0 - Outcome
- contents of the different DNA repeatse0
- Direction
- high degree of heterogeneitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a high degree of heterogeneity in the contents of the different DNA repeats was detected”
t0“tobacco plants”
Claim · model-written summary
Two middle repetitive sequence types were more stable or changed in the same direction.
- Taxon
- tobaccoNicotiana tabacumt0
- Outcome
- contents of middle repetitive sequences of two typese0
- Direction
- more stable or changed in the same directione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“contents of middle repetitive sequences of two types were more stable or changed in the same direction”
t0“tobacco plants”
Claim · model-written summary
The highly repetitive sequence varied independently.
- Taxon
- tobaccoNicotiana tabacumt0
- Outcome
- highly repetitive sequencee0
- Direction
- varied independentlye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the highly repetitive sequence varied independently”
t0“tobacco plants”
Claim · model-written summary
The highly repetitive sequence was significantly reduced versus the two other sequence types.
- Taxon
- tobaccoNicotiana tabacumt0
- Outcome
- highly repetitive sequencee0
- Direction
- reductione0
- Comparator
- the two other sequencese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the highly repetitive sequence varied independently and displayed a significant reduction in comparison with the two other sequences”
t0“tobacco plants”
Claim · model-written summary
The authors infer starting material variation and early in vitro stress were decisive in modified regenerant genomes.
- Taxon
- tobaccoNicotiana tabacumt0
- Outcome
- formation of modified genomes in regenerantse0
- Qualifier
- pre-existing DNA variability in the starting plant material and/or thein vitro stresse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the pre-existing DNA variability in the starting plant material and/or thein vitro stress during the very early stages of protoclone regeneration played a decisive role in the formation of modified genomes in regenerants”
t0“tobacco plants”