Abstract
BACKGROUND: Late blight is the most serious potato disease world-wide. The most effective and environmentally sound way for controlling late blight is to incorporate natural resistance into potato cultivars. Several late blight resistance genes have been cloned recently. However, there is almost no information available about the resistance pathways mediated by any of those genes. RESULTS: We previously cloned a late blight resistance gene, RB, from a diploid wild potato species Solanum bulbocastanum. Transgenic potato lines containing a single RB gene showed a rate-limiting resistance against all known races of Phytophthora infestans, the late blight pathogen. To better understand the RB-mediated resistance we silenced the potato Rar1 and Sgt1 genes that have been implicated in mediating disease resistance responses against various plant pathogens and pests. The Rar1 and Sgt1 genes of a RB-containing potato clone were silenced using a RNA interference (RNAi)-based approach. All of the silenced potato plants displayed phenotypically normal growth. The late blight resistance of the Rar1 and Sgt1 silenced lines were evaluated by a traditional greenhouse inoculation method and quantified using a GFP-tagged P. infestans strain. The resistance of the Rar1-silenced plants was not affected. However, silencing of the Sgt1 gene abolished the RB-mediated resistance. CONCLUSION: Our study shows that silencing of the Sgt1 gene in potato does not result in lethality. However, the Sgt1 gene is essential for the RB-mediated late blight resistance. In contrast, the Rar1 gene is not required for RB-mediated resistance. These results provide additional evidence for the universal role of the Sgt1 gene in various R gene-mediated plant defense responses.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 4
Claim · model-written summary
RB transgenic potato lines resisted all known Phytophthora infestans races.
- Taxon
- potato
Solanum tuberosume0 - Interactor
- Phytophthora infestans
Phytophthora infestanse0pathogen - Gene
- RBe0
- Outcome
- rate-limiting resistancee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transgenic potato lines containing a single RB gene showed a rate-limiting resistance against all known races of Phytophthora infestans”
Claim · model-written summary
Rar1 and Sgt1 silenced potato plants showed normal growth.
- Taxon
- potatoSolanum tuberosume0
- Gene
- Rar1 and Sgt1e1
- Outcome
- phenotypically normal growthe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“All of the silenced potato plants displayed phenotypically normal growth”
e1“The Rar1 and Sgt1 genes of a RB-containing potato clone were silenced”
Claim · model-written summary
Silencing Rar1 did not affect late blight resistance.
- Taxon
- potatoSolanum tuberosumt0
- Treatment
- Rar1-silencede0
- Gene
- Rar1e0
- Outcome
- resistancee0
- Direction
- not affectede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The resistance of the Rar1-silenced plants was not affected”
e1“The Rar1 and Sgt1 genes of a RB-containing potato clone were silenced”
t0“potato late blight”
Claim · model-written summary
Silencing Sgt1 abolished RB-mediated resistance.
- Taxon
- potatoSolanum tuberosumt0
- Treatment
- silencing of the Sgt1 genee0
- Gene
- Sgt1e0
- Outcome
- RB-mediated resistancee0
- Direction
- abolishede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“silencing of the Sgt1 gene abolished the RB-mediated resistance”
t0“potato late blight”
The same questions in other papers
loss of function · disease · Solanum tuberosum (potato)Positive and negative results
11 papers in the same direction as this paper · 15 in the opposite direction
- positive
- positive
- positive
23 papers more in this group
See all 27 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.