Abstract
Keratinase [EC 3.4.21/24/99.11] production by Bacillus pumilus GHD was investigated by solid-state fermentation (SSF) using sugar cane bagasse as a substrate. The SSF conditions were optimised using the “one variable at a time” strategy. A Box-Behnken design was employed to investigate the effects of adding the most common mineral salts on improving enzyme production. Maximal enzyme activity and amino acid production [730 ± 4.9 U/g dry solid (ds) and 368 ± 4.2 mg/g ds, respectively] was achieved using a combination of salts in concentrations of 0.087, 0.120, 0.204 g /10 g ds for NaCl, ZnCl 2 and MgSO 4 , respectively. Capsicum annuum seeds were planted and irrigated with the amino-acid-rich culture filtrate of B. pumilus GHD. Growth rate, number of leaves and pigments (chlorophyll a and carotenoids) increased significantly with a 20-day irrigation at dilutions of 1:5 and 1:10 (v/v). The results of scanning electron microscopy showed the complete degradation of nail and horn upon treatment with the crude enzyme.
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Claims 4
Claim · model-written summary
A 20-day irrigation with 1:5 diluted culture filtrate significantly increased growth rate and leaf number.
- Taxon
- Capsicum annuum
Capsicum annuumt0 - Treatment
- 20-day irrigation at dilutions of 1:5e0
- Outcome
- Growth rate, number of leavese0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Growth rate, number of leaves and pigments (chlorophyll a and carotenoids) increased significantly with a 20-day irrigation at dilutions of 1:5 and 1:10 (v/v)”
t0“Capsicum annuum seeds were planted”
Claim · model-written summary
A 20-day irrigation with 1:5 diluted culture filtrate significantly increased chlorophyll a and carotenoids.
- Taxon
- Capsicum annuumCapsicum annuumt0
- Treatment
- 20-day irrigation at dilutions of 1:5e0
- Outcome
- pigments (chlorophyll a and carotenoids)e0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Growth rate, number of leaves and pigments (chlorophyll a and carotenoids) increased significantly with a 20-day irrigation at dilutions of 1:5 and 1:10 (v/v)”
t0“Capsicum annuum seeds were planted”
Claim · model-written summary
A 20-day irrigation with 1:10 diluted culture filtrate significantly increased growth rate and leaf number.
- Taxon
- Capsicum annuumCapsicum annuumt0
- Treatment
- 20-day irrigation at dilutions of 1:5 and 1:10 (v/v)e0
- Outcome
- Growth rate, number of leavese0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Growth rate, number of leaves and pigments (chlorophyll a and carotenoids) increased significantly with a 20-day irrigation at dilutions of 1:5 and 1:10 (v/v)”
t0“Capsicum annuum seeds were planted”
Claim · model-written summary
A 20-day irrigation with 1:10 diluted culture filtrate significantly increased chlorophyll a and carotenoids.
- Taxon
- Capsicum annuumCapsicum annuumt0
- Treatment
- 20-day irrigation at dilutions of 1:5 and 1:10 (v/v)e0
- Outcome
- pigments (chlorophyll a and carotenoids)e0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Growth rate, number of leaves and pigments (chlorophyll a and carotenoids) increased significantly with a 20-day irrigation at dilutions of 1:5 and 1:10 (v/v)”
t0“Capsicum annuum seeds were planted”
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See all 29 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.