Abstract
An isolate of Pseudomonas aureofaciens from the phylloplane of sugar beet which was chromosomally modified for monitoring purposes by the insertion of two gene cassettes (kmr-xylE and lacZY) was introduced to the phytosphere of spring wheat in a number of experiments and the resulting microbial perturbations quantified. Such studies involving innocuous bacterial isolates can serve as a guide in the assessment of risk associated with the release of functionally modified microorganisms. Introductions of P. aureofaciens on seeds caused large microbial perturbations (up to 2 log units) at the seedling stage on seeds and roots. As the inoculated plants matured (tillering, flowering and ripening), perturbations of total microbial populations were found to be non-significant. Microbial perturbation on maturing wheat roots as a result of seed inoculations with P. aureofaciens could only be detected using more sensitive monitoring procedures describing the Pseudomonas community in terms of colony appearance rate on a selective Pseudomonas medium. Spray applications of the marked P. aureofaciens isolate onto the leaf surface of wheat caused no significant perturbations of the indigenous microbial populations present on the phylloplane.
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Claims 4
Claim · model-written summary
Seed introduction caused microbial perturbations up to 2 log units on seedling seeds and roots.
- Taxon
- wheat
Triticum aestivumt0 - Organ / stage
- seedling stage on seeds and rootse0
- Treatment
- Introductions of P. aureofaciens on seedse0
- Interactor
- P. aureofaciensPseudomonas chlororaphis subsp. aureofaciense0symbiont
- Outcome
- microbial perturbationse0
- Direction
- largee0
- Value
- up to 2 log unitse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Introductions of P. aureofaciens on seeds caused large microbial perturbations (up to 2 log units) at the seedling stage on seeds and roots.”
t0“wheat”
Claim · model-written summary
Total microbial perturbations were nonsignificant as inoculated wheat matured.
- Taxon
- wheatTriticum aestivumt0
- Organ / stage
- tillering, flowering and ripeninge0
- Outcome
- perturbations of total microbial populationse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“As the inoculated plants matured (tillering, flowering and ripening), perturbations of total microbial populations were found to be non-significant.”
t0“wheat”
Claim · model-written summary
Sensitive monitoring detected microbial perturbation on maturing wheat roots after seed inoculation.
- Taxon
- wheatTriticum aestivume0
- Treatment
- seed inoculations with P. aureofaciense0
- Interactor
- P. aureofaciensPseudomonas chlororaphis subsp. aureofaciense0symbiont
- Outcome
- Microbial perturbation on maturing wheat rootse0
- Comparator
- more sensitive monitoring procedurese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Microbial perturbation on maturing wheat roots as a result of seed inoculations with P. aureofaciens could only be detected using more sensitive monitoring procedures describing the Pseudomonas community in terms of colony appearance rate on a selective Pseudomonas medium.”
Claim · model-written summary
Leaf spray application caused no significant perturbation of indigenous phylloplane microbes.
- Taxon
- wheatTriticum aestivume0
- Treatment
- Spray applications of the marked P. aureofaciens isolatee0
- Interactor
- P. aureofaciensPseudomonas chlororaphis subsp. aureofaciense0symbiont
- Outcome
- perturbations of the indigenous microbial populationse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Spray applications of the marked P. aureofaciens isolate onto the leaf surface of wheat caused no significant perturbations of the indigenous microbial populations present on the phylloplane.”
The same questions in other papers
application · soil: biology · Triticum aestivum (wheat)Positive and negative results
2 papers in the same direction as this paper · 19 in the opposite direction
- positive
- positive
- positive
18 papers more in this group
See all 22 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.