Abstract
Phaseolus vulgaris (common bean) is an agronomic important legume crop native to America, where two centres of genetic diversification (GD) are recognised, one in Mesoamerica and the other in the south Andes. Mesoamerican bean accessions have preferential and more efficient nodulation with Rhizobium etli strains carrying the allele nodC type-α, which is predominant in soils of Mesoamerica. It was previously demonstrated that the host nuclear factor NF-YC1, which is involved in nodule formation and rhizobial infection, contributes to this preferential selection and enhances nodulation in the domesticated accession NAG12 from Mesoamerica. Here, we show that both domesticated and wild Mesoamerican beans exhibit higher nodulation performance with a nodC type-α than with a nodC type-δ strain. Transcripts of NF-YC1 significantly increased in roots of these accessions 24 h post-inoculation (hpi) with the nodC type-α strain. On the other hand, accessions from the Andean GD centre formed a higher number of nodules with a strain carrying the nodC type-δ, which is predominant in Andean soils. However, NF-YC1 transcript levels did not exhibit significant changes in Andean accessions upon inoculation with the nodC type-δ strain, at least at 24 hpi. RNA interference (RNAi)-mediated gene silencing of NF-YC1 in the domesticated Andean accession Alubia showed that NF-YC1 or a closely related member of this family is required for nodule formation and bacterial infection, in agreement with observations in Mesoamerican common beans. Isolation and sequencing of the full-length ORF of NF-YC1 from Alubia revealed that it was identical to the sequence previously identified in the Mesoamerican accession NAG12. Interestingly, overexpression of NF-YC1 had a negative impact on nodule formation in the Alubia accession, independently of the R. etli lineage. Our findings suggest that transcriptional and functional variation of NF-YC1 occurs among genetically diverse bean accessions, which might positively or negatively contribute to the fine-tuning mechanisms that regulate nodule formation in the common bean-R. etli symbiosis.
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Claims 7
Claim · model-written summary
Domesticated and wild Mesoamerican beans nodulated better with a nodC type-α strain than with a nodC type-δ strain.
- Taxon
- Phaseolus vulgaris
Phaseolus vulgarist0 - Material
- domesticated and wild Mesoamerican beanse2
- Treatment
- nodC type-αe2
- Interactor
- Rhizobium etliRhizobium etlit0symbiont
- Outcome
- nodulation performancee2
- Direction
- highere2
- Comparator
- nodC type-δ straine2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“accessions of Phaseolus vulgaris during the symbiotic association with Rhizobium etli”
e1“Mesoamerican bean accessions have preferential and more efficient nodulation with Rhizobium etli strains carrying the allele nodC type-α”
e2“both domesticated and wild Mesoamerican beans exhibit higher nodulation performance with a nodC type-α than with a nodC type-δ strain”
Claim · model-written summary
NF-YC1 transcripts increased significantly in roots of bean accessions 24 h after inoculation with the nodC type-α strain.
- Taxon
- Phaseolus vulgarisPhaseolus vulgarist0
- Organ / stage
- rootse3
- Treatment
- nodC type-αe3
- Interactor
- Rhizobium etliRhizobium etlit0symbiont
- Gene
- NF-YC1e3
- Outcome
- Transcripts of NF-YC1e3
- Direction
- increasede3
- Value
- 24 he3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“accessions of Phaseolus vulgaris during the symbiotic association with Rhizobium etli”
e3“Transcripts of NF-YC1 significantly increased in roots of these accessions 24 h post-inoculation (hpi) with the nodC type-α strain”
Claim · model-written summary
Andean bean accessions formed a higher number of nodules with the nodC type-δ strain.
- Taxon
- Phaseolus vulgarisPhaseolus vulgarist0
- Material
- accessions from the Andean GD centree4
- Treatment
- nodC type-δe4
- Interactor
- Rhizobium etliRhizobium etlit0symbiont
- Outcome
- number of nodulese4
- Direction
- highere4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“accessions of Phaseolus vulgaris during the symbiotic association with Rhizobium etli”
e4“accessions from the Andean GD centre formed a higher number of nodules with a strain carrying the nodC type-δ”
Claim · model-written summary
NF-YC1 transcript levels in Andean accessions did not change significantly after inoculation with the nodC type-δ strain.
- Taxon
- Phaseolus vulgarisPhaseolus vulgarist0
- Treatment
- inoculation with the nodC type-δ straine5
- Interactor
- Rhizobium etliRhizobium etlit0symbiont
- Gene
- NF-YC1e5
- Outcome
- NF-YC1 transcript levelse5
- Direction
- did not exhibit significant changese5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“accessions of Phaseolus vulgaris during the symbiotic association with Rhizobium etli”
e5“NF-YC1 transcript levels did not exhibit significant changes in Andean accessions upon inoculation with the nodC type-δ strain, at least at 24 hpi”
Claim · model-written summary
Silencing NF-YC1 in the Andean accession Alubia showed that NF-YC1, or a closely related member of its family, is required for nodule formation and bacterial infection.
- Taxon
- Phaseolus vulgarisPhaseolus vulgarist0
- Material
- Alubiae6
- Treatment
- RNA interference (RNAi)-mediated gene silencing of NF-YC1e6
- Interactor
- Rhizobium etliRhizobium etlit0symbiont
- Gene
- NF-YC1e6
- Outcome
- nodule formation and bacterial infectione6
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“accessions of Phaseolus vulgaris during the symbiotic association with Rhizobium etli”
e6“RNA interference (RNAi)-mediated gene silencing of NF-YC1 in the domesticated Andean accession Alubia showed that NF-YC1 or a closely related member of this family is required for nodule formation and bacterial infection”
Claim · model-written summary
Overexpression of NF-YC1 had a negative impact on nodule formation in the Alubia accession, independently of the R. etli lineage.
- Taxon
- Phaseolus vulgarisPhaseolus vulgarist0
- Material
- Alubiae7
- Treatment
- overexpression of NF-YC1e7
- Interactor
- Rhizobium etliRhizobium etlit0symbiont
- Outcome
- nodule formatione7
- Direction
- negative impacte7
- Qualifier
- independently of the R. etli lineagee7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“accessions of Phaseolus vulgaris during the symbiotic association with Rhizobium etli”
e7“overexpression of NF-YC1 had a negative impact on nodule formation in the Alubia accession, independently of the R. etli lineage”
Claim · model-written summary
The full-length NF-YC1 ORF from Alubia is identical to the sequence previously identified in the Mesoamerican accession NAG12.
- Taxon
- Phaseolus vulgarisPhaseolus vulgarist0
- Material
- Alubiae0
- Gene
- NF-YC1e0
- Outcome
- full-length ORF of NF-YC1e0
- Qualifier
- identical to the sequence previously identified in the Mesoamerican accession NAG12e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“accessions of Phaseolus vulgaris during the symbiotic association with Rhizobium etli”
e0“Isolation and sequencing of the full-length ORF of NF-YC1 from Alubia revealed that it was identical to the sequence previously identified in the Mesoamerican accession NAG12”
The same questions in other papers
loss of function · Rhizobium etli · Phaseolus vulgaris (common bean)Positive and negative results
1 in the opposite direction
- negative
inoculation · Rhizobium etli · Phaseolus vulgaris (common bean)Positive and negative results
5 in the opposite direction
- positive
- positive
- positive
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.