Abstract
Isolated tomato (Lycopersicon esculentum var. Kc 146) fruit mitochondria could be stored successfully in the frozen state without a cryoprotective agent if the mitochondria were frozen quickly by immersion in liquid nitrogen and later thawed quickly at 30 C. Criteria of freezing damage were rate of respiration, adenosine diphosphate to oxygen ratio, and respiratory control ratio. Marked reduction in respiration and loss of respiratory control occurred when mitochondria were transferred from liquid nitrogen to -5, -10, or -18 C for 15 minutes prior to thawing at 30 C. Dimethylsulfoxide (5%) prevented freezing damage when mitochondria were incubated at -5 C but did not prevent freezing damage at -10 or -18 C. Isolated tomato mitochondria show promise as a model system for studying the nature of freezing damage and the mode of action of cryo-protective agents.
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Claims 6
Claim · model-written summary
Rapid freezing in liquid nitrogen and rapid thawing at 30 C allowed storage without a cryoprotectant.
- Taxon
- tomato
Solanum lycopersicume0 - Material
- Kc 146e0
- Organ / stage
- fruit mitochondriae0
- Treatment
- frozen quickly by immersion in liquid nitrogen and later thawed quickly at 30 Ce0
- Outcome
- stored successfully in the frozen statee0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Isolated tomato (Lycopersicon esculentum var. Kc 146) fruit mitochondria could be stored successfully in the frozen state without a cryoprotective agent if the mitochondria were frozen quickly by immersion in liquid nitrogen and later thawed quickly at 30 C.”
Claim · model-written summary
Intermediate storage at -5, -10 or -18 C markedly reduced mitochondrial respiration.
- Taxon
- tomatoSolanum lycopersicume1
- Material
- Kc 146e1
- Organ / stage
- fruit mitochondriae1
- Treatment
- transferred from liquid nitrogen to -5, -10, or -18 C for 15 minutes prior to thawing at 30 Ce0
- Outcome
- respiratione0
- Direction
- Marked reductione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Marked reduction in respiration and loss of respiratory control occurred when mitochondria were transferred from liquid nitrogen to -5, -10, or -18 C for 15 minutes prior to thawing at 30 C.”
e1“Isolated tomato (Lycopersicon esculentum var. Kc 146) fruit mitochondria”
Claim · model-written summary
Intermediate storage at -5, -10 or -18 C caused loss of mitochondrial respiratory control.
- Taxon
- tomatoSolanum lycopersicume1
- Material
- Kc 146e1
- Organ / stage
- fruit mitochondriae1
- Treatment
- transferred from liquid nitrogen to -5, -10, or -18 C for 15 minutes prior to thawing at 30 Ce0
- Outcome
- respiratory controle0
- Direction
- losse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Marked reduction in respiration and loss of respiratory control occurred when mitochondria were transferred from liquid nitrogen to -5, -10, or -18 C for 15 minutes prior to thawing at 30 C.”
e1“Isolated tomato (Lycopersicon esculentum var. Kc 146) fruit mitochondria”
Claim · model-written summary
Five-percent dimethylsulfoxide prevented freezing damage at -5 C.
- Taxon
- tomatoSolanum lycopersicume1
- Material
- Kc 146e1
- Organ / stage
- fruit mitochondriae1
- Treatment
- Dimethylsulfoxide (5%)e0
- Outcome
- freezing damagee0
- Direction
- preventede0
- Qualifier
- at -5 Ce0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Dimethylsulfoxide (5%) prevented freezing damage when mitochondria were incubated at -5 C”
e1“Isolated tomato (Lycopersicon esculentum var. Kc 146) fruit mitochondria”
Claim · model-written summary
Five-percent dimethylsulfoxide did not prevent freezing damage at -10 or -18 C.
- Taxon
- tomatoSolanum lycopersicume1
- Material
- Kc 146e1
- Organ / stage
- fruit mitochondriae1
- Treatment
- Dimethylsulfoxide (5%)e0
- Outcome
- freezing damagee0
- Direction
- did not prevente0
- Qualifier
- at -10 or -18 Ce0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Dimethylsulfoxide (5%) prevented freezing damage when mitochondria were incubated at -5 C but did not prevent freezing damage at -10 or -18 C.”
e1“Isolated tomato (Lycopersicon esculentum var. Kc 146) fruit mitochondria”
Claim · model-written summary
Isolated tomato mitochondria show promise as a model of freezing damage and cryoprotectant action.
- Taxon
- tomatoSolanum lycopersicume0
- Outcome
- model system for studying the nature of freezing damage and the mode of action of cryo-protective agentse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Isolated tomato mitochondria show promise as a model system for studying the nature of freezing damage and the mode of action of cryo-protective agents.”