Abstract
Nuclei fromZea mays L. root tip meristematic cells were treated according to the conventional method for nuclear matrix isolation and according to a recently adapted procedure for isolation of nuclear shells from plant cells. In the first case, after high salt extraction of proteins and DNase I and RNase digestions, residual structures are obtained consisting of a periferal layer and an internal network. The obtained structures are very similar to the nuclear matrix preparations from animal cells. In case nuclei are swollen in EDTA first, digested with DNase II and RNase prior high salt treatment, structures devoid of internal network are obtained. The analogous residual structures were shown forPhaseolus vulgaris L. meristematic root cells nuclei (Galcheva-Gargovaet al. 1988). The morphology and the protein composition of the two types of residual structures suggest that the organization of scaffold structures from plant nuclei is very similar to the one from animal cell nuclei.
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Claims 3
Claim · model-written summary
Conventional extraction left a peripheral layer and internal network in maize nuclei.
Zea maysOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“residual structures are obtained consisting of a periferal layer and an internal network”
t0“Zea mays L.”
Claim · model-written summary
EDTA swelling followed by digestion and salt treatment left structures without an internal network.
- Outcome
- structures devoid of internal networke0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“structures devoid of internal network are obtained”
t0“Zea mays L.”
Claim · model-written summary
The two maize nuclear residual structures resembled animal nuclear matrix preparations and suggested similar scaffold organization.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The morphology and the protein composition of the two types of residual structures suggest that the organization of scaffold structures from plant nuclei is very similar to the one from animal cell nuclei”
t0“Zea mays L.”