Abstract
Isolation of the four major peroxidase isozymes (isozymes 1, 2, 3, and 4) of two flax genotypes was achieved by modifying the procedure used by Shannon et al. (1966) for the isolation of horseradish peroxidase isozymes. The net positive and net negative charges of isozymes 1, 2, and 4 were different. Isozyme 3 resembled isozyme 4 in charge but differed in apparent molecular weight. The chromatographic elution profiles of both genotypes were the same. Anionic gel electrophoresis demonstrated that after isolation and repurification, relative mobility differences existed between the corresponding isozymes of the two genotypes for all four isozymes.
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Claims 5
Claim · model-written summary
Four major peroxidase isozymes were isolated from two flax genotypes by a modified procedure.
- Taxon
- flax
Linum usitatissimume0 - Gene
- peroxidase isozymese0
- Outcome
- Isolation of the four major peroxidase isozymese0
- Value
- foure0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Isolation of the four major peroxidase isozymes (isozymes 1, 2, 3, and 4) of two flax genotypes was achieved by modifying the procedure used by Shannon et al. (1966) for the isolation of horseradish peroxidase isozymes.”
Claim · model-written summary
Isozymes 1, 2, and 4 differed in net charge.
- Taxon
- flaxLinum usitatissimumt0
- Gene
- isozymes 1, 2, and 4e0
- Outcome
- net positive and net negative chargese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The net positive and net negative charges of isozymes 1, 2, and 4 were different.”
t0“flax genotypes”
Claim · model-written summary
Isozyme 3 resembled isozyme 4 in charge but differed in apparent molecular weight.
- Taxon
- flaxLinum usitatissimumt0
- Gene
- Isozyme 3e0
- Outcome
- charge but differed in apparent molecular weighte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Isozyme 3 resembled isozyme 4 in charge but differed in apparent molecular weight.”
t0“flax genotypes”
Claim · model-written summary
Chromatographic elution profiles were the same for both flax genotypes.
- Taxon
- flaxLinum usitatissimumt0
- Outcome
- chromatographic elution profilese0
- Direction
- samee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The chromatographic elution profiles of both genotypes were the same.”
t0“flax genotypes”
Claim · model-written summary
Corresponding isozymes of the two flax genotypes differed in relative mobility after isolation and repurification.
- Taxon
- flaxLinum usitatissimumt0
- Gene
- isozymese0
- Outcome
- relative mobility differencese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Anionic gel electrophoresis demonstrated that after isolation and repurification, relative mobility differences existed between the corresponding isozymes of the two genotypes for all four isozymes.”
t0“flax genotypes”