Abstract
The replication of shuttle vectors derived from Wheat Dwarf Virus, a monopartite geminivirus, was studied in cultured maize endosperm cells, and in the Black Mexican Sweet (BMS) maize cell line. Using in vivo labeling and DNA methylation analysis, we showed that replication was initiated within 24 hrs after transfection, and did not require cell division in both cell lines. Copy numbers of 30,000 ds DNA molecules per cell were observed in endosperm cells after three days. The replication protein was shown to act in trans, since the wild type gene of the shuttle vector enabled replication-deficient vectors carrying mutated genes to replicate. These properties suggest that WDV may have similar applications in plants as SV40 in mammalian cells.
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Claims 5
Claim · model-written summary
Vector replication began within 24 hours after transfection.
Zea maysOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“replication was initiated within 24 hrs after transfection”
t0“maize cells”
Claim · model-written summary
Vector replication did not require cell division.
- Outcome
- did not require cell divisione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“did not require cell division in both cell lines”
t0“maize cells”
Claim · model-written summary
Endosperm cells reached 30,000 double-stranded DNA molecules per cell after three days.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Copy numbers of 30,000 ds DNA molecules per cell were observed in endosperm cells after three days.”
t0“maize cells”
Claim · model-written summary
The replication protein acted in trans to enable replication-deficient vectors to replicate.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The replication protein was shown to act in trans, since the wild type gene of the shuttle vector enabled replication-deficient vectors carrying mutated genes to replicate.”
t0“maize cells”
Claim · model-written summary
Authors suggested wheat dwarf virus could have plant vector applications.
- Taxon
- plantsViridiplantaee0
- Outcome
- applications in plantse0
Verbatim
e0“These properties suggest that WDV may have similar applications in plants as SV40 in mammalian cells.”