Abstract
When a crude extract from 8-day-old wheat (Triticum aestivum L. cv. Olympic) leaves was fractionated by a combination of ammonium sulfate precipitation and Sephadex G-100 chromatography the presence of three factors which have a marked effect on the stability of highly purified nitrate reductase was revealed. Two of these factors (I and III) have a positive effect and the other factor (II) has a negative effect on stability. Factors I and III can each overcome the instability-promoting effect of II; however, this was apparently not due to a direct effect on factor II.Both factors I and III have been subjected to further purification. Factor I can be separated into at least four fractions, each with stability-promoting activity. Factor III appears to be a single factor.The in vitro activity and stability of nitrate reductase in crude extracts were found to vary diurnally. Stability and activity were highest 4 hours after the start of the light period and both were minimal 1 to 3 hours after the end of the light period. When crude extract was fractionated as described above and an assessment made of the relative amounts of I, II, and III, there appeared to be a distinct diurnal variation in their levels. Factors I and III were highest when in vitro nitrate reductase activity and stability were highest. Factor II was apparently out of phase in that maximum activity coincided with the time of minimum in vitro nitrate reductase activity and stability.
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Claims 8
Claim · model-written summary
Factors I and III promoted purified nitrate reductase stability.
- Taxon
- Triticum aestivum L.
Triticum aestivume1 - Gene
- nitrate reductasee2
- Outcome
- stabilitye0
- Direction
- positive effecte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Two of these factors (I and III) have a positive effect and the other factor (II) has a negative effect on stability.”
e1“wheat (Triticum aestivum L. cv. Olympic) leaves”
e2“highly purified nitrate reductase”
Claim · model-written summary
Factor II reduced purified nitrate reductase stability.
- Taxon
- Triticum aestivum L.Triticum aestivume2
- Gene
- nitrate reductasee1
- Outcome
- stabilitye0
- Direction
- negative effecte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Two of these factors (I and III) have a positive effect and the other factor (II) has a negative effect on stability.”
e1“highly purified nitrate reductase”
e2“wheat (Triticum aestivum L. cv. Olympic) leaves”
Claim · model-written summary
Factors I and III each overcame the instability promoting action of factor II, apparently without acting directly on II.
- Taxon
- Triticum aestivum L.Triticum aestivume1
- Outcome
- instability-promoting effect of IIe0
- Direction
- overcomee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Factors I and III can each overcome the instability-promoting effect of II; however, this was apparently not due to a direct effect on factor II.”
e1“wheat (Triticum aestivum L. cv. Olympic) leaves”
Claim · model-written summary
Factor I separated into at least four stability promoting fractions.
- Taxon
- Triticum aestivum L.Triticum aestivume1
- Outcome
- fractionse0
- Value
- at least foure0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Factor I can be separated into at least four fractions, each with stability-promoting activity.”
e1“wheat (Triticum aestivum L. cv. Olympic) leaves”
Claim · model-written summary
Factor III appeared to be a single factor.
- Taxon
- Triticum aestivum L.Triticum aestivume1
- Outcome
- single factore0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Factor III appears to be a single factor.”
e1“wheat (Triticum aestivum L. cv. Olympic) leaves”
Claim · model-written summary
Nitrate reductase activity and stability varied daily, peaking four hours after light began and reaching minima one to three hours after light ended.
- Taxon
- Triticum aestivum L.Triticum aestivume1
- Gene
- nitrate reductasee2
- Outcome
- Stability and activitye0
- Direction
- higheste0
- Value
- 4 hourse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Stability and activity were highest 4 hours after the start of the light period and both were minimal 1 to 3 hours after the end of the light period.”
e1“wheat (Triticum aestivum L. cv. Olympic) leaves”
e2“nitrate reductase in crude extracts”
Claim · model-written summary
Factors I and III peaked when nitrate reductase activity and stability peaked.
- Taxon
- Triticum aestivum L.Triticum aestivume1
- Gene
- nitrate reductasee0
- Outcome
- in vitro nitrate reductase activity and stabilitye0
- Direction
- higheste0
- Qualifier
- Factors I and III were higheste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Factors I and III were highest when in vitro nitrate reductase activity and stability were highest.”
e1“wheat (Triticum aestivum L. cv. Olympic) leaves”
Claim · model-written summary
Factor II activity peaked when nitrate reductase activity and stability were minimal.
- Taxon
- Triticum aestivum L.Triticum aestivume1
- Gene
- nitrate reductasee0
- Outcome
- in vitro nitrate reductase activity and stabilitye0
- Direction
- minimume0
- Qualifier
- maximum activitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Factor II was apparently out of phase in that maximum activity coincided with the time of minimum in vitro nitrate reductase activity and stability.”
e1“wheat (Triticum aestivum L. cv. Olympic) leaves”