Abstract
In November 2002, samples were received from an all-year-round chrysanthemum crop growing under glass in south-west England. The crop consisted of cvs Fiji and Calabria, cuttings of which originated from Brazil. The samples had distinct dark stem lesions and some leaf necrosis. Given these symptoms and the absence of any fungal pathogens, the plants were tested by ELISA for the presence of three tospoviruses. Chrysanthemum stem necrosis virus (CSNV) was detected using a polyclonal DAS-ELISA kit (DSMZ, Braunschweig, Germany). Neither Tomato spotted wilt virus nor Impatiens necrotic spot virus were detected. When examined electron-microscopically, sap from the samples was found to contain spherical tospovirus-like virus particles of 90–110 nm in diameter. Mechanical inoculation of sap from affected stem material to a range of indicator plant species resulted in symptoms typical of CSNV (Bezerra et al., 1999), including systemic mosaic in Nicotiana benthamiana; these indicator plants showing symptoms tested positive for CSNV when tested by ELISA. RT-PCR using degenerate tospovirus primers BR60 and BR65 (Eiras et al., 2001) was also performed on extracts taken from chrysanthemum stem tissue showing symptoms and a single product of the correct predicted size (453 bp) was obtained; this product was sequenced and shown to share 98% identity with the 5′ end of the capsid protein gene of a published CSNV sequence (AF067068). The infected crop has now been removed and measures taken to eradicate the infection. As CSNV has previously been reported only from Brazil and the Netherlands (Bezerra et al., 1999;Verhoeven et al., 1996), this is the first report of its occurrence in the UK.
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Claims 8
Claim · model-written summary
Symptomatic chrysanthemum samples had dark stem lesions and leaf necrosis.
- Taxon
- chrysanthemum
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Chrysanthemumt0 - Outcome
- dark stem lesions and some leaf necrosise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
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Verbatim
e0“The samples had distinct dark stem lesions and some leaf necrosis.”
t0“Chrysanthemum”
Claim · model-written summary
CSNV was detected in symptomatic chrysanthemum by ELISA.
- Taxon
- ChrysanthemumChrysanthemume0
- Interactor
- Chrysanthemum stem necrosis virus (CSNV)Chrysanthemum stem necrosis viruse0pathogen
- Outcome
- Chrysanthemum stem necrosis virus (CSNV) was detectede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Chrysanthemum stem necrosis virus (CSNV) was detected using a polyclonal DAS-ELISA kit”
Claim · model-written summary
Tomato spotted wilt virus was not detected in the chrysanthemum samples.
- Taxon
- chrysanthemumChrysanthemumt0
- Interactor
- Tomato spotted wilt virus
Orthotospovirus tomatomaculaee0pathogen - Outcome
- Tomato spotted wilt viruse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Neither Tomato spotted wilt virus nor Impatiens necrotic spot virus were detected.”
t0“Chrysanthemum”
Claim · model-written summary
Impatiens necrotic spot virus was not detected in the chrysanthemum samples.
- Taxon
- chrysanthemumChrysanthemumt0
- Interactor
- Impatiens necrotic spot virus
Impatiens necrotic spot viruse0pathogen - Outcome
- Impatiens necrotic spot viruse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Neither Tomato spotted wilt virus nor Impatiens necrotic spot virus were detected.”
t0“Chrysanthemum”
Claim · model-written summary
Sap from chrysanthemum samples contained tospovirus-like particles 90–110 nm in diameter.
- Taxon
- chrysanthemumChrysanthemumt0
- Outcome
- spherical tospovirus-like virus particlese0
- Value
- 90–110 nme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“sap from the samples was found to contain spherical tospovirus-like virus particles of 90–110 nm in diameter”
t0“Chrysanthemum”
Claim · model-written summary
Sap inoculation produced systemic mosaic in Nicotiana benthamiana, with CSNV positive ELISA tests.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae0 - Treatment
- Mechanical inoculation of sap from affected stem materiale0
- Interactor
- CSNVe0pathogen
- Outcome
- systemic mosaice0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mechanical inoculation of sap from affected stem material to a range of indicator plant species resulted in symptoms typical of CSNV (Bezerra et al., 1999), including systemic mosaic in Nicotiana benthamiana; these indicator plants showing symptoms tested positive for CSNV when tested by ELISA.”
Claim · model-written summary
RT-PCR of symptomatic chrysanthemum stem yielded a 453 bp product with 98% identity to a CSNV capsid gene sequence.
- Taxon
- chrysanthemumChrysanthemumt0
- Interactor
- CSNVe0pathogen
- Outcome
- a single product of the correct predicted sizee0
- Value
- 453 bpe0
- Qualifier
- 98% identitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a single product of the correct predicted size (453 bp) was obtained; this product was sequenced and shown to share 98% identity with the 5′ end of the capsid protein gene of a published CSNV sequence”
t0“Chrysanthemum”
Claim · model-written summary
The infected chrysanthemum crop was the first reported UK occurrence of CSNV.
- Taxon
- ChrysanthemumChrysanthemumt0
- Outcome
- first report of its occurrence in the UKe0
- Setting
- UKe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“this is the first report of its occurrence in the UK.”
t0“First report of Chrysanthemum stem necrosis virus (CSNV) in the UK”
The same questions in other papers
inoculation · disease · Nicotiana benthamianaPositive and negative results
7 papers in the same direction as this paper · 31 in the opposite direction · 1 reporting no difference from an alternative
- negative
- negative
- negative
36 papers more in this group
See all 40 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.