Abstract
The fluorescent labeling/HPSEC method was developed and the molar-based distribution of amylopectin unit-chains, and molecules of amylose and amylopectin from various sources was examined. The molar-based distribution of A and B chains enabled the estimation of the number of chains per cluster. Cereal amylopectins had a larger number of chains in a cluster than root and tuber amylopectins. A-Crystalline type starches contained amylopectin with a larger number of chains per cluster than B-type crystalline. The molar distribution of C chain suggested the presence of long and short C-chains, which appeared to connect two clusters and were in single cluster, respectively. Amylopectin comprised three molecular species. The large species was major in amount by mole and weight, but the medium and small species were relatively large in amount by mole but very small by weight. The three species appeared to be built up with a similar cluster in structure and differed in the number of clusters. The number of clusters suggested that the large and medium species might be blocklets in granule while the small species immature and/or degraded products of the large species. Amylose comprised several molecular species with different size, and their proportions differed by plant sources. The small amylose species was predominant in cereal amyloses while the large amylose species in root and tuber amyloses. The number-average degree of polymerization of amyloses and the number average chain length of amylopectins determined by the labeling/HPSEC method were in good agreement with those determined by conventional colorimetric methods.
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Claims 13
Claim · model-written summary
The molar distribution of A and B chains enabled estimation of chains per cluster.
- Outcome
- estimation of the number of chains per clustere0
Not reportedValue
Verbatim
e0“The molar-based distribution of A and B chains enabled the estimation of the number of chains per cluster.”
Claim · model-written summary
Cereal amylopectins had more chains per cluster than root and tuber amylopectins.
Verbatim
e0“Cereal amylopectins had a larger number of chains in a cluster than root and tuber amylopectins.”
Claim · model-written summary
A-crystalline starch amylopectin had more chains per cluster than B-type crystalline starch amylopectin.
Verbatim
e0“A-Crystalline type starches contained amylopectin with a larger number of chains per cluster than B-type crystalline.”
Claim · model-written summary
C chains appeared to include long and short forms connecting two clusters and occurring in a single cluster, respectively.
- Outcome
- presence of long and short C-chainse0
- Qualifier
- appeared to connect two clusters and were in single cluster, respectivelye0
Not reportedTaxon
Verbatim
e0“The molar distribution of C chain suggested the presence of long and short C-chains, which appeared to connect two clusters and were in single cluster, respectively.”
Claim · model-written summary
Amylopectin comprised three molecular species.
- Outcome
- three molecular speciese0
Not reportedTaxon
Verbatim
e0“Amylopectin comprised three molecular species.”
Claim · model-written summary
The large amylopectin species was major by mole and weight.
Not reportedTaxon
Verbatim
e0“The large species was major in amount by mole and weight”
Claim · model-written summary
Medium and small amylopectin species were relatively large by mole but very small by weight.
Not reportedTaxon
Verbatim
e0“the medium and small species were relatively large in amount by mole but very small by weight.”
Claim · model-written summary
Three amylopectin species appeared to have similar cluster structure and different numbers of clusters.
- Outcome
- similar cluster in structuree0
Not reportedTaxon
Verbatim
e0“The three species appeared to be built up with a similar cluster in structure and differed in the number of clusters.”
Claim · model-written summary
The large and medium species may be granule blocklets and small species immature or degraded products.
- Outcome
- blocklets in granulee0
Not reportedTaxon
Verbatim
e0“the large and medium species might be blocklets in granule while the small species immature and/or degraded products of the large species.”
Claim · model-written summary
Amylose comprised multiple molecular species of different sizes whose proportions differed by plant source.
Verbatim
e0“Amylose comprised several molecular species with different size, and their proportions differed by plant sources.”
Claim · model-written summary
Small amylose species predominated in cereals.
Verbatim
e0“The small amylose species was predominant in cereal amyloses”
Claim · model-written summary
Large amylose species predominated in root and tuber sources.
Verbatim
e0“the large amylose species in root and tuber amyloses.”
Claim · model-written summary
Labeling/HPSEC estimates agreed with conventional colorimetric estimates.
- Outcome
- number-average degree of polymerization of amyloses and the number average chain length of amylopectinse0
- Comparator
- conventional colorimetric methodse0
Not reportedTaxon
Verbatim
e0“The number-average degree of polymerization of amyloses and the number average chain length of amylopectins determined by the labeling/HPSEC method were in good agreement with those determined by conventional colorimetric methods.”