Abstract
AUXIN RESPONSE FACTOR7 (ARF7) is one of five ARF transcriptional activators in Arabidopsis thaliana that is proposed to regulate auxin-responsive expression of genes containing TGTCTC auxin response elements in their promoters. An Arabidopsis mutant (nonphototropic hypocotyl4-1 [nph4-1]) that is a null for ARF7 showed strongly reduced expression of integrated auxin-responsive reporter genes and natural genes that were monitored in Arabidopsis leaf mesophyll protoplasts. Expression of the reporter and natural genes was restored in an auxin-dependent manner when protoplasts were transfected with a 35S:ARF7 effector gene, encoding a full-length ARF7 protein. Transfection of effector genes encoding other ARF activators restored auxin-responsive gene expression to varying degrees, but less than that observed with the ARF7 effector gene. Arabidopsis lines that were null for ARF6, ARF8, or ARF19 were not defective in expression of the reporter and natural auxin response genes assayed in mesophyll protoplasts, suggesting that ARF7 plays a major role in regulating expression of a subset of auxin response genes in leaf mesophyll cells. Auxin-responsive gene expression was induced in wild-type protoplasts and restored in nph4-1 protoplasts only with auxin and not with other hormones, including brassinolide. In the presence of auxin, however, brassinolide modestly enhanced auxin-responsive gene expression.
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Claims 7
Claim · model-written summary
The ARF7-null nph4-1 mutant had strongly reduced auxin-responsive reporter and natural gene expression in Arabidopsis leaf mesophyll protoplasts.
- Taxon
- Arabidopsis
Arabidopsise0 - Material
- nonphototropic hypocotyl4-1 [nph4-1]e0
- Organ / stage
- leaf mesophyll protoplastse0
- Gene
- ARF7e0
- Outcome
- expression of integrated auxin-responsive reporter genes and natural genese0
- Direction
- strongly reducede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“An Arabidopsis mutant (nonphototropic hypocotyl4-1 [nph4-1]) that is a null for ARF7 showed strongly reduced expression of integrated auxin-responsive reporter genes and natural genes that were monitored in Arabidopsis leaf mesophyll protoplasts.”
Claim · model-written summary
Transfection with 35S:ARF7 restored reporter and natural gene expression in an auxin-dependent manner.
- Treatment
- transfected with a 35S:ARF7 effector genee0
- Gene
- ARF7e0
- Outcome
- Expression of the reporter and natural genese0
- Direction
- restorede0
- Qualifier
- auxin-dependente0
Not reportedTaxonComparator
Verbatim
e0“Expression of the reporter and natural genes was restored in an auxin-dependent manner when protoplasts were transfected with a 35S:ARF7 effector gene, encoding a full-length ARF7 protein.”
Claim · model-written summary
Other ARF activator effector genes restored auxin-responsive expression less than the ARF7 effector gene.
- Treatment
- effector genes encoding other ARF activatorse0
- Outcome
- auxin-responsive gene expressione0
- Direction
- restorede0
- Qualifier
- less than that observed with the ARF7 effector genee0
- Comparator
- ARF7 effector genee0
Not reportedTaxon
Verbatim
e0“Transfection of effector genes encoding other ARF activators restored auxin-responsive gene expression to varying degrees, but less than that observed with the ARF7 effector gene.”
Claim · model-written summary
ARF6-, ARF8-, and ARF19-null Arabidopsis lines were not defective in auxin response gene expression in mesophyll protoplasts.
- Taxon
- ArabidopsisArabidopsise0
- Organ / stage
- mesophyll protoplastse0
- Gene
- ARF6, ARF8, or ARF19e0
- Outcome
- expression of the reporter and natural auxin response genese0
- Direction
- not defectivee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis lines that were null for ARF6, ARF8, or ARF19 were not defective in expression of the reporter and natural auxin response genes assayed in mesophyll protoplasts”
Claim · model-written summary
Auxin induced auxin-responsive gene expression in wild-type protoplasts.
- Material
- wild-typee0
- Treatment
- auxine0
- Outcome
- Auxin-responsive gene expressione0
- Direction
- inducede0
- Comparator
- other hormones, including brassinolidee0
Not reportedTaxon
Verbatim
e0“Auxin-responsive gene expression was induced in wild-type protoplasts and restored in nph4-1 protoplasts only with auxin and not with other hormones, including brassinolide.”
Claim · model-written summary
Auxin restored auxin-responsive gene expression in nph4-1 protoplasts, unlike other hormones including brassinolide.
- Material
- nph4-1e0
- Treatment
- auxine0
- Outcome
- Auxin-responsive gene expressione0
- Direction
- restorede0
- Comparator
- other hormones, including brassinolidee0
Not reportedTaxon
Verbatim
e0“Auxin-responsive gene expression was induced in wild-type protoplasts and restored in nph4-1 protoplasts only with auxin and not with other hormones, including brassinolide.”
Claim · model-written summary
Brassinolide modestly enhanced auxin-responsive gene expression when auxin was present.
- Treatment
- brassinolidee0
- Outcome
- auxin-responsive gene expressione0
- Direction
- enhancede0
- Qualifier
- In the presence of auxine0
Not reportedTaxonComparator
Verbatim
e0“In the presence of auxin, however, brassinolide modestly enhanced auxin-responsive gene expression.”
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See all 75 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.