Abstract
Evaluation of genetic relationships is of prime importance in any breeding studies. The present study was carried out to estimate the genetic diversity of Brassica napus germplasm using randomly amplified polymorphic DNA (RAPD) primers. A total of 16 B. napus genotypes were evaluated using ten RAPD primers. Bivariate data matrix was generated and genetic distances were calculated using unweighted pair group of arithmetic mean (UPGMA) procedure, which amplified 2.31, 2.70, 1.14, 2.46, 1.82, 2.24, 1.78, 3.06, 2.25 and 2.46 alleles per genotype, respectively. All the genotypes showed various levels of genetic polymorphism for the loci detected by using RAPD primers. A total of 217 alleles (bands) were amplified in 16 genotypes using 10 primers giving the average of 13.56 alleles per primer. Among the ten primers used in the present study, primer GLA-09 yielded an average minimum number of bands (1.14), while primer GLD-08 yielded maximum (3.06) number of alleles per genotype on average. Level of genetic polymorphism (estimated as percent genetic distance) observed during the study varied, but on average, and it was in the range of 19 to 93%. Maximum genetic distances were observed among PR-102 and Dunkled, closely followed by PS-1 and PR-111. These findings were further strengthened by dendrogram analyses. Genetically distinct lines pointed out in the present study (PR-102 and Dunkled) could be used in future breeding programs for developing better quality canola inbred lines/varieties. Key words: Genetic diversity, randomly amplified polymorphic DNA (RAPD),Brassica napus, rapeseed.
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Claims 6
Claim · model-written summary
RAPD primers amplified 217 alleles across 16 B. napus genotypes
- Taxon
- Brassica napus
_Brassica_napus_-_Habit.jpg?width=60)
Brassica napust0 - Outcome
- alleles (bands) were amplifiede1
- Value
- 217e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Brassica_napus_-_Habit.jpg?width=500)
Verbatim
t0“Characterization of Brassica napus germplasm based on molecular markers”
e1“A total of 217 alleles (bands) were amplified in 16 genotypes using 10 primers giving the average of 13.56 alleles per primer”
Claim · model-written summary
Genetic polymorphism of the B. napus genotypes ranged from 19 to 93% genetic distance
- Taxon
- Brassica napusBrassica napust0
- Outcome
- genetic polymorphisme2
- Value
- 19 to 93%e2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Characterization of Brassica napus germplasm based on molecular markers”
e2“Level of genetic polymorphism (estimated as percent genetic distance) observed during the study varied, but on average, and it was in the range of 19 to 93%”
Claim · model-written summary
Primer GLD-08 gave the maximum number of alleles per genotype (3.06)
- Taxon
- Brassica napusBrassica napust0
- Outcome
- number of alleles per genotypee3
- Value
- 3.06e3
- Qualifier
- primer GLD-08e3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Characterization of Brassica napus germplasm based on molecular markers”
e3“Among the ten primers used in the present study, primer GLA-09 yielded an average minimum number of bands (1.14), while primer GLD-08 yielded maximum (3.06) number of alleles per genotype on average”
Claim · model-written summary
Primer GLA-09 gave the minimum number of bands (1.14)
- Taxon
- Brassica napusBrassica napust0
- Outcome
- number of bandse3
- Value
- 1.14e3
- Qualifier
- primer GLA-09e3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Characterization of Brassica napus germplasm based on molecular markers”
e3“Among the ten primers used in the present study, primer GLA-09 yielded an average minimum number of bands (1.14), while primer GLD-08 yielded maximum (3.06) number of alleles per genotype on average”
Claim · model-written summary
Maximum genetic distances were observed between PR-102 and Dunkled
- Taxon
- Brassica napusBrassica napust0
- Material
- PR-102 and Dunklede4
- Outcome
- Maximum genetic distancese4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Characterization of Brassica napus germplasm based on molecular markers”
e4“Maximum genetic distances were observed among PR-102 and Dunkled, closely followed by PS-1 and PR-111”
Claim · model-written summary
PR-102 and Dunkled are recommended for future breeding of canola inbred lines
- Taxon
- canola
.jpg?width=60)
Brassica napus var. napuse5 - Material
- PR-102 and Dunklede5
- Outcome
- used in future breeding programse5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e5“Genetically distinct lines pointed out in the present study (PR-102 and Dunkled) could be used in future breeding programs for developing better quality canola inbred lines/varieties”