Abstract
A survey of the Arabidopsis thaliana databases revealed that single C2H2 zinc finger protein genes comprise a large gene family (approximately 30 genes). No known phenotype has been associated with any of these genes except SUPERMAN. One of these genes, designated AtZFP10 (A. thaliana single zinc finger protein), was isolated by RT-PCR in the present study. The AtZFP10 gene was expressed at low levels in the flowers, axillary meristems and siliques, and at very low levels in the stems in Arabidopsis. Overexpression of the AtZFP10 gene driven by a constitutive promoter resulted in abnormal Arabidopsis plants and only one plant was recovered. Tobacco plants overexpressing the AtZFP10 gene displayed dwarfing, abnormal leaf phenotypes and early flowering that correlated with the level of expression of the AtZFP10 gene. No differences were observed in cell size between the AtZFP10 transgenic plants and the wild-type plants. Application of exogenous GA3 did not restore the wild-type phenotype, but it did reduce the dwarfing phenotype. Deletion of the leucine-rich region at the carboxyl terminus of the AtZFP10 gene resulted in transgenic plants that were not phenotypically different from wild-type plants suggesting a role for the leucine-rich region as essential for normal function.
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Claims 6
Claim · model-written summary
The AtZFP10 gene is expressed at low levels in Arabidopsis flowers, axillary meristems and siliques, and at very low levels in stems.
- Taxon
- Arabidopsis
Arabidopsise1 - Organ / stage
- flowers, axillary meristems and siliquese1
- Gene
- AtZFP10e1
- Outcome
- expressed at low levelse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The AtZFP10 gene was expressed at low levels in the flowers, axillary meristems and siliques, and at very low levels in the stems in Arabidopsis”
Claim · model-written summary
Tobacco plants overexpressing AtZFP10 showed dwarfing, abnormal leaf phenotypes and early flowering relative to wild-type plants.
- Taxon
- Tobacco
Nicotiana tabacume3 - Treatment
- overexpressing the AtZFP10 genee3
- Gene
- AtZFP10e3
- Outcome
- dwarfing, abnormal leaf phenotypes and early floweringe3
- Qualifier
- correlated with the level of expressione3
- Comparator
- wild-type plantse4
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“Tobacco plants overexpressing the AtZFP10 gene displayed dwarfing, abnormal leaf phenotypes and early flowering that correlated with the level of expression of the AtZFP10 gene”
e4“No differences were observed in cell size between the AtZFP10 transgenic plants and the wild-type plants”
Claim · model-written summary
Exogenous GA3 did not restore the wild-type phenotype of AtZFP10 tobacco plants.
- Taxon
- TobaccoNicotiana tabacume3
- Treatment
- exogenous GA3e5
- Outcome
- wild-type phenotypee5
- Direction
- did not restoree5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“Tobacco plants overexpressing the AtZFP10 gene displayed dwarfing, abnormal leaf phenotypes and early flowering that correlated with the level of expression of the AtZFP10 gene”
e5“Application of exogenous GA3 did not restore the wild-type phenotype”
Claim · model-written summary
Exogenous GA3 reduced the dwarfing phenotype of AtZFP10 tobacco plants.
- Taxon
- TobaccoNicotiana tabacume3
- Treatment
- exogenous GA3e5
- Outcome
- dwarfing phenotypee6
- Direction
- reducee6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“Tobacco plants overexpressing the AtZFP10 gene displayed dwarfing, abnormal leaf phenotypes and early flowering that correlated with the level of expression of the AtZFP10 gene”
e5“Application of exogenous GA3 did not restore the wild-type phenotype”
e6“but it did reduce the dwarfing phenotype”
Claim · model-written summary
Deleting the leucine-rich region at the carboxyl terminus of AtZFP10 gave transgenic plants not phenotypically different from wild-type plants.
- Treatment
- Deletion of the leucine-rich region at the carboxyl terminus of the AtZFP10 genee7
- Gene
- AtZFP10e7
- Outcome
- phenotypically differente7
- Direction
- not phenotypically differente7
- Comparator
- wild-type plantse7
Not reportedTaxon
Verbatim
e7“Deletion of the leucine-rich region at the carboxyl terminus of the AtZFP10 gene resulted in transgenic plants that were not phenotypically different from wild-type plants”
Claim · model-written summary
Overexpressing AtZFP10 under a constitutive promoter produced abnormal Arabidopsis plants, and only one plant was recovered.
- Taxon
- ArabidopsisArabidopsise2
- Treatment
- Overexpression of the AtZFP10 gene driven by a constitutive promotere2
- Gene
- AtZFP10e2
- Outcome
- abnormal Arabidopsis plantse2
- Qualifier
- only one plant was recoverede2
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“Overexpression of the AtZFP10 gene driven by a constitutive promoter resulted in abnormal Arabidopsis plants and only one plant was recovered”