Paper · 2014 · AFRICAN JOURNAL OF BIOTECHNOLOGY

Analysis and identification of oils from seed extract of Anthonotha macrophylla using gas chromatography-mass spectrometry (GC-MS)

K. C. Ugoeze,; T. Ehianeta,; C. Alaribe,; et al.

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verbatim quotes
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citations
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Open access · cc-by

Abstract

The volatile components obtained from the seed extract of Anthonotha Macrophylla were analyzed using gas chromatography-mass spectrometry (GC-MS). Prior to GC-MS analysis, Soxhelt extraction was carried out from the seeds of A. Macrophylla. Agilent GC-MS system comprising 6890GC model coupled with 5973 n mass selective detector was used for analysis. The GC is equipped with Agilent 7673 autosampler and a 30 m 0.25 id DB-1 MS dimethylpolysiolaxane capillary column. The MS source temperature was set at 230C and electron energy at 70V. The ionisation mode was electron ionization and the mass range was 50 to 550 while the scan time was 1 scan/min. The different compounds were identified by matching their mass spectra with the MS spectra in the NIST library. Various compounds were separated and identified but eight of these were at an elevated level. These include: n-hexadecanoic acid, n-octadecadienoic acid; cis-vaccenic acid; octadecanoic acid; hexadecanoic acid 2-hydroxy-1-(hydroxymethyl) ethyl ester; campesterol; stigmasterol and gamma-sitosterol. Key words: Gas chromatography-mass spectrometry, determination, evaluation, composition, Anthonotha macrophylla, seed oil.

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Claims 4

Observationobservedoriginal resultmeasured

Claim · model-written summary

Eight compounds were identified at elevated levels in Anthonotha macrophylla seed extract.

Taxon
Anthonotha macrophyllaAnthonotha macrophyllat0
Organ / stage
seedt0
Outcome
eight of these were at an elevated levele0

Verbatim

e0

“Various compounds were separated and identified but eight of these were at an elevated level.”

✓ located verbatim in the source · abstract, characters 764–857 · show in text ↑
t0

“seed extract of Anthonotha macrophylla”

✓ located verbatim in the source · title, characters 41–79 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

The elevated seed constituents included n-hexadecanoic acid, n-octadecadienoic acid and cis-vaccenic acid.

Taxon
Anthonotha macrophyllaAnthonotha macrophyllat0
Organ / stage
seedt0
Outcome
n-hexadecanoic acid, n-octadecadienoic acid; cis-vaccenic acide0

Verbatim

e0

“These include: n-hexadecanoic acid, n-octadecadienoic acid; cis-vaccenic acid”

✓ located verbatim in the source · abstract, characters 858–935 · show in text ↑
t0

“seed extract of Anthonotha macrophylla”

✓ located verbatim in the source · title, characters 41–79 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

The elevated seed constituents included octadecanoic acid and hexadecanoic acid 2-hydroxy-1-(hydroxymethyl) ethyl ester.

Taxon
Anthonotha macrophyllaAnthonotha macrophyllat0
Organ / stage
seedt0
Outcome
octadecanoic acid; hexadecanoic acid 2-hydroxy-1-(hydroxymethyl) ethyl estere0

Verbatim

e0

“octadecanoic acid; hexadecanoic acid 2-hydroxy-1-(hydroxymethyl) ethyl ester”

✓ located verbatim in the source · abstract, characters 937–1013 · show in text ↑
t0

“seed extract of Anthonotha macrophylla”

✓ located verbatim in the source · title, characters 41–79 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

The elevated seed constituents included campesterol, stigmasterol and gamma-sitosterol.

Taxon
Anthonotha macrophyllaAnthonotha macrophyllat0
Organ / stage
seedt0
Outcome
campesterol; stigmasterol and gamma-sitosterole0

Verbatim

e0

“campesterol; stigmasterol and gamma-sitosterol.”

✓ located verbatim in the source · abstract, characters 1015–1062 · show in text ↑
t0

“seed extract of Anthonotha macrophylla”

✓ located verbatim in the source · title, characters 41–79 · show in text ↑