Paper · 1973 · Physiologia Plantarum

The Light Induced Protochlorophyll–Chlorophyll a‐Transformation and the Succeeding Interconversions of the Different Forms of Chlorophyll

HEMMING I. VIRGIN; C. STACY FRENCH

4
claims
7
verbatim quotes
33
citations
2
research areas
—
Not open access

Abstract

Abstract Curve resolution into Gaussian components of the absorption spectra during the varying stages of the Shibata shift in dark grown, irradiated leaves of barley indicates that the chlorophyll a forms formed after irradiation consist of the same main components which have been reported to be present in all hitherto investigated plant materials (peak values in the red region 662, 670, 677 and 683 nm, respectively) but in varying proportions. The spectra during the Shibata shift proper can be satisfied by a mixture of two single components gradually changing their proportions, although a four component system gives a still better fit to the measured absorption curves. It is also shown that curves taken before and after the shift and added together in the appropriate proportions will match the absorption spectrum measured with peak at the isosbestic point (after ca. 15 min at room temperature).

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Claims 4

Observationobservedoriginal resultmeasured

Claim · model-written summary

Irradiated barley leaves contained four main chlorophyll a spectral components with red peaks at 662, 670, 677 and 683 nm in varying proportions.

Organ / stage
leavese0
Treatment
irradiatede0
Outcome
chlorophyll a forms formed after irradiatione0
Value
662, 670, 677 and 683 nme0
Qualifier
varying proportionse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“dark grown, irradiated leaves of barley indicates that the chlorophyll a forms formed after irradiation consist of the same main components which have been reported to be present in all hitherto investigated plant materials (peak values in the red region 662, 670, 677 and 683 nm, respectively) but in varying proportions.”

✓ located verbatim in the source · abstract, characters 127–449 · show in text ↑
Method / resourceobservedoriginal resultmeasured

Claim · model-written summary

A two-component mixture with changing proportions fits the spectra during the Shibata shift.

Outcome
spectra during the Shibata shift propere0
Value
two single componentse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The spectra during the Shibata shift proper can be satisfied by a mixture of two single components gradually changing their proportions”

✓ located verbatim in the source · abstract, characters 450–585 · show in text ↑
e1

“leaves of barley”

✓ located verbatim in the source · abstract, characters 150–166 · show in text ↑
Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

A four-component system fits measured absorption curves better.

Outcome
fit to the measured absorption curvese0
Direction
bettere0
Value
four component systeme0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“although a four component system gives a still better fit to the measured absorption curves.”

✓ located verbatim in the source · abstract, characters 587–679 · show in text ↑
e1

“leaves of barley”

✓ located verbatim in the source · abstract, characters 150–166 · show in text ↑
Method / resourceobservedoriginal resultmeasured

Claim · model-written summary

Weighted pre- and post-shift curves match the spectrum at the isosbestic point after about 15 minutes.

Outcome
match the absorption spectrum measured with peak at the isosbestic pointe0
Value
ca. 15 mine0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“curves taken before and after the shift and added together in the appropriate proportions will match the absorption spectrum measured with peak at the isosbestic point (after ca. 15 min at room temperature).”

✓ located verbatim in the source · abstract, characters 702–909 · show in text ↑
e1

“leaves of barley”

✓ located verbatim in the source · abstract, characters 150–166 · show in text ↑