Abstract
A simple and rapid micellar electrokinetic chromatography method was developed for simultaneous determination of indole-3-acetic acid, indole-3-butyric acid, gibberellic acid, abscisic acid and naphthylacetic acid in mungbean sprouts for monitoring plant growth and development. The effects of several parameters related to the separation and determination were investigated in detail. The analysis was carried out using 10 mM borax, 10 mM sodium dihydrogen phosphate, 90 mM sodium dodecyl sulfate and 5% acetonitrile as running buffer (pH 9.0). Under optimum conditions, the method demonstrated good performance concerning linearity (r, 0.9954-0.9991), precision (0.77-4.97%), the method limit of detection (LOD) and the method limit of quantitation (LOQ) (LOD, 0.011-0.177 mg/kg; LOQ, 0.035-0.590 mg/kg) and accuracy (83.62-102.56%). The results confirmed that the method is rapid, convenient and of low cost for the determination of the phytohormones.
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Claims 6
Claim · model-written summary
The method showed good linearity (r 0.9954-0.9991) for determining phytohormones in mungbean sprouts.
- Taxon
- Mungbean
.jpg?width=60)
Vigna radiatat0 - Outcome
- linearitye1
- Value
- 0.9954-0.9991e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
t0“Mungbean Sprouts”
e1“good performance concerning linearity (r, 0.9954-0.9991)”
Claim · model-written summary
The method showed precision of 0.77-4.97% for determining phytohormones in mungbean sprouts.
- Taxon
- MungbeanVigna radiatat0
- Outcome
- precisione2
- Value
- 0.77-4.97%e2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Mungbean Sprouts”
e2“precision (0.77-4.97%)”
Claim · model-written summary
The method limit of detection (LOD) for phytohormones in mungbean sprouts was 0.011-0.177 mg/kg.
- Taxon
- MungbeanVigna radiatat0
- Outcome
- LODe3
- Value
- 0.011-0.177 mg/kge3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Mungbean Sprouts”
e3“LOD, 0.011-0.177 mg/kg”
Claim · model-written summary
The method limit of quantitation (LOQ) for phytohormones in mungbean sprouts was 0.035-0.590 mg/kg.
- Taxon
- MungbeanVigna radiatat0
- Outcome
- LOQe4
- Value
- 0.035-0.590 mg/kge4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Mungbean Sprouts”
e4“LOQ, 0.035-0.590 mg/kg”
Claim · model-written summary
The method showed accuracy of 83.62-102.56% for determining phytohormones in mungbean sprouts.
- Taxon
- MungbeanVigna radiatat0
- Outcome
- accuracye5
- Value
- 83.62-102.56%e5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Mungbean Sprouts”
e5“accuracy (83.62-102.56%)”
Claim · model-written summary
The authors conclude that the method is rapid, convenient and of low cost for determining phytohormones in mungbean sprouts.
- Taxon
- MungbeanVigna radiatat0
- Outcome
- rapid, convenient and of low coste6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Mungbean Sprouts”
e6“The results confirmed that the method is rapid, convenient and of low cost for the determination of the phytohormones”