Abstract
Soybean seeds are non-sterile and their bacterial population interferes with the enumeration of beneficial bacteria, making it difficult to assess survival under different conditions. Within this context, the principal aims of this work were: (1) to improve a selective media for the enumeration of B. japonicum recovered from inoculated soybean seeds; (2) to establish the most representative mathematical function for B. japonicum mortality on soybean seeds after inoculation; (3) to evaluate if environmental or physiological conditions modify B. japonicum mortality on soybean seeds; and (4) to create a new protocol for quality control of soybean inoculants. We successfully evaluated the combination of pentachloronitrobenzene and vancomycin added to the yeast-mannitol medium to inhibit most fungi and Gram-positive soybean microbiota, thus producing reliable counts of B. japonicum from inoculated soybean seeds. Percentages of recovery and survival factors were obtained and used to construct a two-phase exponential decay non-linear regression function. High temperature and desiccation decreased these parameters, while the optimization of temperature and the use of osmoprotective compounds with inoculants increased them. The use of this protocol minimized heterogeneity between experiments and may be considered more reliable than the simple expression of direct colony count of bacteria recovered from seeds.
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Claims 4
Claim · model-written summary
The selective medium with pentachloronitrobenzene and vancomycin gives reliable counts of B. japonicum from inoculated soybean seeds, more reliable than direct colony counts.
- Taxon
- Soybean
Glycine maxe0 - Interactor
- B. japonicume1symbiont
- Outcome
- reliable counts of B. japonicum from inoculated soybean seedse3
- Comparator
- direct colony count of bacteria recovered from seedse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Soybean seeds are non-sterile”
e1“enumeration of B. japonicum recovered from inoculated soybean seeds”
e2“than the simple expression of direct colony count of bacteria recovered from seeds”
e3“producing reliable counts of B. japonicum from inoculated soybean seeds”
Claim · model-written summary
High temperature and desiccation decreased the recovery and survival factors of B. japonicum on soybean seeds.
- Taxon
- SoybeanGlycine maxe0
- Treatment
- High temperature and desiccatione4
- Interactor
- B. japonicume1symbiont
- Outcome
- survival factorse5
- Direction
- decreasede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Soybean seeds are non-sterile”
e1“enumeration of B. japonicum recovered from inoculated soybean seeds”
e4“High temperature and desiccation decreased these parameters”
e5“Percentages of recovery and survival factors were obtained”
Claim · model-written summary
Optimized temperature and osmoprotective compounds increased the recovery and survival factors of B. japonicum on soybean seeds.
- Taxon
- SoybeanGlycine maxe0
- Treatment
- the optimization of temperature and the use of osmoprotective compounds with inoculantse6
- Interactor
- B. japonicume1symbiont
- Outcome
- survival factorse5
- Direction
- increasede6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Soybean seeds are non-sterile”
e1“enumeration of B. japonicum recovered from inoculated soybean seeds”
e6“the optimization of temperature and the use of osmoprotective compounds with inoculants increased them”
e5“Percentages of recovery and survival factors were obtained”
Claim · model-written summary
The proposed protocol minimized heterogeneity between experiments and may be more reliable than direct colony counts.
- Taxon
- SoybeanGlycine maxe0
- Outcome
- heterogeneity between experimentse7
- Comparator
- direct colony count of bacteria recovered from seedse7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Soybean seeds are non-sterile”
e7“The use of this protocol minimized heterogeneity between experiments and may be considered more reliable than the simple expression of direct colony count of bacteria recovered from seeds”
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1 in the opposite direction
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inoculation · survival · Glycine max (soybean)Positive and negative results
4 papers in the same direction as this paper · 3 in the opposite direction
- negative
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See all 8 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.