Abstract
BACKGROUND: Potato virus Y (PVY) is a major pathogen that causes substantial economic losses in worldwide potato production. Different potato cultivars differ in resistance to PVY, from severe susceptibility, through tolerance, to complete resistance. The aim of this study was to better define the mechanisms underlying tolerant responses of potato to infection by the particularly aggressive PVY(NTN) strain. We focused on the dynamics of the primary metabolism-related processes during PVY(NTN) infection. RESULTS: A comprehensive analysis of the dynamic changes in primary metabolism was performed, which included whole transcriptome analysis, nontargeted proteomics, and photosynthetic activity measurements in potato cv. Désirée and its transgenic counterpart depleted for accumulation of salicylic acid (NahG-Désirée). Faster multiplication of virus occurred in the NahG-Désirée, with these plants developing strong disease symptoms. We show that while the dynamics of responses at the transcriptional level are extensive and bimodal, this is only partially translated to the protein level, and to the final functional outcome. Photosynthesis-related genes are transiently induced before viral multiplication is detected and it is down-regulated later on. This is reflected as a deficiency of the photosynthetic apparatus at the onset of viral multiplication only. Interestingly, specific and constant up-regulation of some RuBisCO transcripts was detected in Désirée plants, which might be important, as these proteins have been shown to interact with viral proteins. In SA-deficient and more sensitive NahG-Désirée plants, consistent down-regulation of photosynthesis-related genes was detected. A constant reduction in the photochemical efficiency from the onset of viral multiplication was identified; in nontransgenic plants this decrease was only transient. The transient reduction in net photosynthetic rate occurred in both genotypes with the same timing, and coincided with changes in stomatal conductivity. CONCLUSIONS: Down-regulation of photosynthesis-related gene expression and decreased photosynthetic activity is in line with other studies that have reported the effects of biotic stress on photosynthesis. Here, we additionally detected induction of light-reaction components in the early stages of PVY(NTN) infection of tolerant interaction. As some of these components have already been shown to interact with viral proteins, their overproduction might contribute to the absence of symptoms in cv. Désirée.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 14
Claim · model-written summary
Faster virus multiplication occurred in NahG-Désirée potato than in Désirée during PVY(NTN) infection
- Taxon
- potato
Solanum tuberosumt0 - Material
- NahG-Désiréee1
- Treatment
- PVY(NTN) infectione2
- Interactor
- Potato virus Y
Potato virus Yt0pathogen - Outcome
- multiplication of viruse1
- Direction
- Fastere1
- Comparator
- Désiréee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tolerant potato-Potato virus Y interaction”
e1“Faster multiplication of virus occurred in the NahG-Désirée, with these plants developing strong disease symptoms”
e2“We focused on the dynamics of the primary metabolism-related processes during PVY(NTN) infection”
Claim · model-written summary
NahG-Désirée potato plants developed strong disease symptoms
- Taxon
- potatoSolanum tuberosumt0
- Material
- NahG-Désiréee1
- Outcome
- strong disease symptomse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tolerant potato-Potato virus Y interaction”
e1“Faster multiplication of virus occurred in the NahG-Désirée, with these plants developing strong disease symptoms”
Claim · model-written summary
Photosynthesis-related genes were transiently induced before viral multiplication was detected
- Taxon
- potatoSolanum tuberosumt0
- Gene
- Photosynthesis-related genese3
- Outcome
- transiently inducede3
- Qualifier
- before viral multiplication is detectede3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tolerant potato-Potato virus Y interaction”
e3“Photosynthesis-related genes are transiently induced before viral multiplication is detected and it is down-regulated later on”
Claim · model-written summary
Photosynthesis-related genes were down-regulated later in PVY(NTN) infection
- Taxon
- potatoSolanum tuberosumt0
- Gene
- Photosynthesis-related genese3
- Outcome
- down-regulatede3
- Qualifier
- later one3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tolerant potato-Potato virus Y interaction”
e3“Photosynthesis-related genes are transiently induced before viral multiplication is detected and it is down-regulated later on”
Claim · model-written summary
A deficiency of the photosynthetic apparatus was present at the onset of viral multiplication only
- Taxon
- potatoSolanum tuberosumt0
- Outcome
- deficiency of the photosynthetic apparatuse4
- Qualifier
- at the onset of viral multiplication onlye4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tolerant potato-Potato virus Y interaction”
e4“This is reflected as a deficiency of the photosynthetic apparatus at the onset of viral multiplication only”
Claim · model-written summary
Specific and constant up-regulation of some RuBisCO transcripts was detected in Désirée potato
- Taxon
- potatoSolanum tuberosumt0
- Material
- Désiréee5
- Gene
- RuBisCO transcriptse5
- Outcome
- up-regulation of some RuBisCO transcriptse5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tolerant potato-Potato virus Y interaction”
e5“specific and constant up-regulation of some RuBisCO transcripts was detected in Désirée plants”
Claim · model-written summary
In NahG-Désirée plants, consistent down-regulation of photosynthesis-related genes was detected
- Taxon
- potatoSolanum tuberosumt0
- Material
- NahG-Désiréee6
- Gene
- photosynthesis-related genese6
- Outcome
- consistent down-regulation of photosynthesis-related genese6
- Qualifier
- SA-deficient and more sensitivee6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tolerant potato-Potato virus Y interaction”
e6“In SA-deficient and more sensitive NahG-Désirée plants, consistent down-regulation of photosynthesis-related genes was detected”
Claim · model-written summary
Photochemical efficiency showed a constant reduction from the onset of viral multiplication
- Taxon
- potatoSolanum tuberosumt0
- Outcome
- photochemical efficiencye7
- Qualifier
- from the onset of viral multiplicatione7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tolerant potato-Potato virus Y interaction”
e7“A constant reduction in the photochemical efficiency from the onset of viral multiplication was identified”
Claim · model-written summary
In nontransgenic potato plants, the reduction in photochemical efficiency was only transient
- Taxon
- potatoSolanum tuberosumt0
- Material
- nontransgenic plantse0
- Outcome
- photochemical efficiencye7
- Qualifier
- only transiente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tolerant potato-Potato virus Y interaction”
e7“A constant reduction in the photochemical efficiency from the onset of viral multiplication was identified”
e0“in nontransgenic plants this decrease was only transient”
Claim · model-written summary
Net photosynthetic rate showed a transient reduction in both genotypes at the same timing
- Taxon
- potatoSolanum tuberosumt0
- Material
- both genotypese0
- Outcome
- net photosynthetic ratee0
- Qualifier
- transient reductione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tolerant potato-Potato virus Y interaction”
e0“The transient reduction in net photosynthetic rate occurred in both genotypes with the same timing, and coincided with changes in stomatal conductivity”
Claim · model-written summary
Changes in stomatal conductivity coincided with the transient reduction in net photosynthetic rate
- Taxon
- potatoSolanum tuberosumt0
- Material
- both genotypese0
- Outcome
- stomatal conductivitye0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tolerant potato-Potato virus Y interaction”
e0“The transient reduction in net photosynthetic rate occurred in both genotypes with the same timing, and coincided with changes in stomatal conductivity”
Claim · model-written summary
Induction of light-reaction components was detected in the early stages of PVY(NTN) infection in the tolerant interaction
- Taxon
- potatoSolanum tuberosumt0
- Outcome
- induction of light-reaction componentse0
- Qualifier
- in the early stages of PVY(NTN) infectione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tolerant potato-Potato virus Y interaction”
e0“Here, we additionally detected induction of light-reaction components in the early stages of PVY(NTN) infection of tolerant interaction”
Claim · model-written summary
Dynamics of responses at the transcriptional level were extensive and bimodal
- Taxon
- potatoSolanum tuberosumt0
- Outcome
- dynamics of responses at the transcriptional levele0
- Qualifier
- extensive and bimodale0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tolerant potato-Potato virus Y interaction”
e0“We show that while the dynamics of responses at the transcriptional level are extensive and bimodal, this is only partially translated to the protein level, and to the final functional outcome”
Claim · model-written summary
Transcriptional responses were only partially translated to the protein level
- Taxon
- potatoSolanum tuberosumt0
- Outcome
- translated to the protein levele0
- Qualifier
- only partiallye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tolerant potato-Potato virus Y interaction”
e0“We show that while the dynamics of responses at the transcriptional level are extensive and bimodal, this is only partially translated to the protein level, and to the final functional outcome”
The same questions in other papers
infection · Potato virus Y · Solanum tuberosum (potato)Positive and negative results
11 papers in the same direction as this paper · 3 in the opposite direction
- negative
- positive
- positive
11 papers more in this group
See all 15 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.