Abstract
The effect of progressive 5[prime] deletions within a potato proteinase inhibitor II promoter on wound-inducible expression of the chloramphenicol acetyltransferase (CAT) gene in leaves of transgenic tobacco plants was analyzed. After deletion of a region ranging from position -1300 to -700 with respect to the transcription start site, promoter activity was markedly reduced but still wound-inducible. Further deletion of approximately 200 base pairs resulted in a promoter activity that was below the detection limit, proving that the activity of the proteinase inhibitor II promoter is controlled by sequences upstream of position -514. Addition of the enhancer of the 35S promoter of the cauliflower mosaic virus (CaMV) either 5[prime] upstream or 3[prime] downstream of chimeric genes consisting of different proteinase inhibitor II promoter deletions (-700, -514, -210) fused to the CAT gene led to wound-inducible CAT gene expression in a fraction of transgenic plants containing either the "-700" or "-514" promoters, indicating the presence of wound-responsive elements in the promoter-proximal region. A fragment of the proteinase inhibitor II promoter comprising sequences between positions -1300 and -195 is able to confer wound-inducible expression to an inactive CaMV 35S promoter truncated at position -90 in either orientation, proving that this fragment displays wound-specific, enhancer-like properties. In addition, data are presented excluding that the proteinase inhibitor II 3[prime] end is of importance for wound-inducible gene expression.
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Claims 5
Claim · model-written summary
Deleting promoter positions -1300 to -700 markedly reduced activity while retaining wound inducibility.
- Taxon
- Tobacco
Nicotiana tabacumt0 - Treatment
- deletion of a region ranging from position -1300 to -700e0
- Gene
- proteinase inhibitor IIe1
- Outcome
- promoter activitye0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“After deletion of a region ranging from position -1300 to -700 with respect to the transcription start site, promoter activity was markedly reduced but still wound-inducible”
e1“potato proteinase inhibitor II promoter”
t0“Transgenic Tobacco Plants”
Claim · model-written summary
A further approximately 200-base-pair deletion lowered promoter activity below detection.
- Taxon
- TobaccoNicotiana tabacumt0
- Treatment
- Further deletion of approximately 200 base pairse0
- Outcome
- promoter activitye0
- Direction
- below the detection limite0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Further deletion of approximately 200 base pairs resulted in a promoter activity that was below the detection limit”
t0“Tobacco Plants”
Claim · model-written summary
The CaMV 35S enhancer yielded wound-inducible CAT expression with -700 or -514 promoters in a fraction of plants.
- Taxon
- TobaccoNicotiana tabacumt0
- Treatment
- Addition of the enhancer of the 35S promoter of the cauliflower mosaic virus (CaMV)e0
- Gene
- CAT genee0
- Outcome
- wound-inducible CAT gene expressione0
- Qualifier
- a fraction of transgenic plants containing either the "-700" or "-514" promoterse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Addition of the enhancer of the 35S promoter of the cauliflower mosaic virus (CaMV) either 5[prime] upstream or 3[prime] downstream of chimeric genes consisting of different proteinase inhibitor II promoter deletions (-700, -514, -210) fused to the CAT gene led to wound-inducible CAT gene expression in a fraction of transgenic plants containing either the "-700" or "-514" promoters”
t0“Tobacco Plants”
Claim · model-written summary
The -1300 to -195 promoter fragment conferred wound-inducible expression to inactive CaMV 35S promoter in either orientation.
- Taxon
- TobaccoNicotiana tabacumt0
- Treatment
- A fragment of the proteinase inhibitor II promoter comprising sequences between positions -1300 and -195e0
- Gene
- proteinase inhibitor II promotere0
- Outcome
- wound-inducible expressione0
- Comparator
- inactive CaMV 35S promoter truncated at position -90e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A fragment of the proteinase inhibitor II promoter comprising sequences between positions -1300 and -195 is able to confer wound-inducible expression to an inactive CaMV 35S promoter truncated at position -90 in either orientation”
t0“Tobacco Plants”
Claim · model-written summary
The proteinase inhibitor II 3-prime end was not important for wound-inducible expression.
- Taxon
- TobaccoNicotiana tabacumt0
- Gene
- proteinase inhibitor II 3[prime] ende0
- Outcome
- wound-inducible gene expressione0
- Direction
- excludinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“excluding that the proteinase inhibitor II 3[prime] end is of importance for wound-inducible gene expression”
t0“Tobacco Plants”
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