Abstract
ST-LS1 is a light-inducible, single-copy gene from potato that is expressed only in photosynthetic tissues. Various sequences derived from the 5'-upstream region of this gene were fused to the coding region of the chloramphenicol acetyltransferase (CAT) gene and to the gene 7 termination region of the transfer DNA (T-DNA) from the Agrobacterium Ti plasmid pTiACH5 and transferred to tobacco using Ti-plasmid vectors. After regeneration of whole plants, tissues were assayed for the expression of the CAT gene. Sequences derived from the 5'-upstream region of the ST-LS1 gene comprising positions -334 to +11 were sufficient to confer a leaf/stem-specific as well as a light-inducible expression of the CAT gene. Destruction of chloroplasts by treatment with the herbicide norfluorazon and subsequent exposure to light drastically reduced the expression of the CAT gene indicating that this upstream sequence most likely interacts with a chloroplast-dependent signal. When sequences from position -98 to position +675 were fused to a truncated inactive fragment of the cauliflower mosaic virus 35S promoter in a head-to-head manner, the corresponding chimeric genes were again expressed in photosynthetic tissues only, indicating that these sequences have enhancer-like properties.
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Claims 7
Claim · model-written summary
Potato ST-LS1 is light inducible and expressed only in photosynthetic tissues.
- Taxon
- potato
Solanum tuberosume0 - Organ / stage
- photosynthetic tissuese0
- Gene
- ST-LS1e0
- Outcome
- expressed only in photosynthetic tissuese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ST-LS1 is a light-inducible, single-copy gene from potato that is expressed only in photosynthetic tissues”
Claim · model-written summary
The ST-LS1 upstream region from -334 to +11 conferred leaf and stem specific CAT expression in tobacco.
- Taxon
- tobacco
Nicotiana tabacume1 - Organ / stage
- leaf/steme0
- Treatment
- Sequences derived from the 5'-upstream region of the ST-LS1 gene comprising positions -334 to +11e0
- Gene
- ST-LS1e0
- Outcome
- leaf/stem-specifice0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Sequences derived from the 5'-upstream region of the ST-LS1 gene comprising positions -334 to +11 were sufficient to confer a leaf/stem-specific as well as a light-inducible expression of the CAT gene”
t0“potato gene in transgenic plants”
e1“transferred to tobacco using Ti-plasmid vectors”
Claim · model-written summary
The same ST-LS1 upstream region conferred light-inducible CAT expression in tobacco.
- Taxon
- tobaccoNicotiana tabacume1
- Treatment
- Sequences derived from the 5'-upstream region of the ST-LS1 gene comprising positions -334 to +11e0
- Gene
- ST-LS1e0
- Outcome
- light-inducible expression of the CAT genee0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Sequences derived from the 5'-upstream region of the ST-LS1 gene comprising positions -334 to +11 were sufficient to confer a leaf/stem-specific as well as a light-inducible expression of the CAT gene”
e1“transferred to tobacco using Ti-plasmid vectors”
Claim · model-written summary
Norfluorazon chloroplast destruction followed by light drastically reduced CAT expression.
- Taxon
- tobaccoNicotiana tabacume1
- Treatment
- treatment with the herbicide norfluorazon and subsequent exposure to lighte0
- Gene
- CAT genee0
- Outcome
- expression of the CAT genee0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Destruction of chloroplasts by treatment with the herbicide norfluorazon and subsequent exposure to light drastically reduced the expression of the CAT gene”
e1“transferred to tobacco using Ti-plasmid vectors”
Claim · model-written summary
The chloroplast treatment result suggested that the ST-LS1 upstream sequence interacts with a chloroplast-dependent signal.
- Taxon
- tobaccoNicotiana tabacume1
- Outcome
- interacts with a chloroplast-dependent signale0
- Qualifier
- most likelye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“indicating that this upstream sequence most likely interacts with a chloroplast-dependent signal”
e1“transferred to tobacco using Ti-plasmid vectors”
Claim · model-written summary
The -98 to +675 sequences conferred expression of chimeric genes only in photosynthetic tissues.
- Taxon
- tobaccoNicotiana tabacume1
- Organ / stage
- photosynthetic tissuese0
- Treatment
- sequences from position -98 to position +675 were fused to a truncated inactive fragment of the cauliflower mosaic virus 35S promotere0
- Outcome
- chimeric genes were again expressed in photosynthetic tissues onlye0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When sequences from position -98 to position +675 were fused to a truncated inactive fragment of the cauliflower mosaic virus 35S promoter in a head-to-head manner, the corresponding chimeric genes were again expressed in photosynthetic tissues only”
e1“transferred to tobacco using Ti-plasmid vectors”
Claim · model-written summary
The -98 to +675 sequences showed enhancer-like properties.
- Taxon
- tobaccoNicotiana tabacume2
- Outcome
- enhancer-like propertiese0
- Qualifier
- sequences from position -98 to position +675e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“these sequences have enhancer-like properties”
e1“sequences from position -98 to position +675”
e2“transferred to tobacco using Ti-plasmid vectors”
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- positive
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- positive
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11 papers more in this group
See all 15 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.