Abstract
Mutant tobacco plants deficient for class I beta-1,3-glucanase (GLU I) are decreased in their susceptibility to virus infection. This is correlated with delayed virus spread, a reduction in the size exclusion limit of plasmodesmata and increased cell-wall deposition of the beta-1,3-glucan callose. To further investigate a role of GLU I during cell-to-cell movement of virus infection, we inserted the GLU I coding sequence into TMV for overexpression in infected cells. Compared with the size of local lesions produced on plants infected with virus expressing either an enzymatically inactive GLU I or a frameshift mutant of the gene, the size of local lesions caused by infection with virus expressing active GLU I was consistently increased. Viruses expressing antisense GLU I constructs led to lesions of decreased size. Similar effects were obtained for virus spread using plants grown at 32 degrees C to block the hypersensitive response. Together, these results indicate that enzymatically active GLU I expressed in cells containing replicating virus can increase cell-to-cell movement of virus. This supports the view that GLU I induced locally during infection helps to promote cell-to-cell movement of virus by hydrolyzing callose. Moreover, our results provide the first direct evidence that a biological function of a plant beta-1,3-glucanase depends on its catalytic activity.
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Claims 5
Claim · model-written summary
Virus expressing active GLU I increased the size of local lesions in tobacco compared with inactive GLU I or a frameshift mutant.
- Taxon
- tobacco
Nicotiana tabacume0 - Treatment
- virus expressing active GLU Ie2
- Interactor
- TMV
Tobacco mosaic viruse1pathogen - Gene
- GLU Ie2
- Outcome
- size of local lesionse2
- Direction
- increasede2
- Comparator
- virus expressing either an enzymatically inactive GLU I or a frameshift mutant of the genee2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mutant tobacco plants deficient for class I beta-1,3-glucanase (GLU I)”
e1“we inserted the GLU I coding sequence into TMV for overexpression in infected cells”
e2“Compared with the size of local lesions produced on plants infected with virus expressing either an enzymatically inactive GLU I or a frameshift mutant of the gene, the size of local lesions caused by infection with virus expressing active GLU I was consistently increased”
Claim · model-written summary
Antisense GLU I constructs in the virus led to lesions of decreased size in tobacco.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- antisense GLU I constructse3
- Gene
- GLU Ie3
- Outcome
- lesionse3
- Direction
- decreasede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mutant tobacco plants deficient for class I beta-1,3-glucanase (GLU I)”
e3“Viruses expressing antisense GLU I constructs led to lesions of decreased size”
Claim · model-written summary
Similar effects on virus spread were obtained in tobacco plants grown at 32 degrees C.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- plants grown at 32 degrees Ce4
- Outcome
- virus spreade4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mutant tobacco plants deficient for class I beta-1,3-glucanase (GLU I)”
e4“Similar effects were obtained for virus spread using plants grown at 32 degrees C to block the hypersensitive response”
Claim · model-written summary
GLU I induced locally during infection is proposed to promote cell-to-cell virus movement by hydrolyzing callose.
- Taxon
- tobaccoNicotiana tabacume0
- Gene
- GLU Ie5
- Outcome
- cell-to-cell movement of viruse5
- Qualifier
- hydrolyzing callosee5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mutant tobacco plants deficient for class I beta-1,3-glucanase (GLU I)”
e5“This supports the view that GLU I induced locally during infection helps to promote cell-to-cell movement of virus by hydrolyzing callose”
Claim · model-written summary
A biological function of a plant beta-1,3-glucanase depends on its catalytic activity.
- Taxon
- tobaccoNicotiana tabacume0
- Gene
- beta-1,3-glucanasee6
- Outcome
- biological functione6
- Direction
- depends one6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mutant tobacco plants deficient for class I beta-1,3-glucanase (GLU I)”
e6“a biological function of a plant beta-1,3-glucanase depends on its catalytic activity”
The same questions in other papers
loss of function · disease · Nicotiana tabacum (tobacco)Positive and negative results
20 papers in the same direction as this paper · 35 in the opposite direction
- positive
- negative
- negative
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See all 56 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.