Abstract
Susceptibility to virus infection is decreased in a class I beta-1,3-glucanase (GLU I)-deficient mutant (TAG4.4) of tobacco generated by antisense transformation. TAG4.4 exhibited delayed intercellular trafficking via plasmodesmata of a tobamovirus (tobacco mosaic virus), of a potexvirus (recombinant potato virus X expressing GFP), and of the movement protein (MP) 3a of a cucumovirus (cucumber mosaic virus). Monitoring the cell-to-cell movement of dextrans and peptides by a novel biolistic method revealed that the plasmodesmatal size exclusion limit (SEL) of TAG4.4 was also reduced from 1.0 to 0.85 nm. Therefore, GLU I-deficiency has a broad effect on plasmodesmatal movement, which is not limited to a particular virus type. Deposition of callose, a substrate for beta-1,3-glucanases, was increased in TAG4.4 in response to 32 degrees C treatment, treatment with the fungal elicitor xylanase, and wounding, suggesting that GLU I has an important function in regulating callose metabolism. Callose turnover is thought to regulate plasmodesmatal SEL. We propose that GLU I induction in response to infection may help promote MP-driven virus spread by degrading callose.
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Claims 9
Claim · model-written summary
Virus susceptibility decreased in tobacco GLU I deficient TAG4.4.
- Taxon
- tobacco
Nicotiana tabacume0 - Material
- TAG4.4e0
- Treatment
- antisense transformatione0
- Gene
- GLU Ie0
- Outcome
- Susceptibility to virus infectione0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Susceptibility to virus infection is decreased in a class I beta-1,3-glucanase (GLU I)-deficient mutant (TAG4.4) of tobacco generated by antisense transformation”
Claim · model-written summary
TAG4.4 delayed plasmodesmatal trafficking of tobacco mosaic virus.
- Material
- TAG4.4e0
- Interactor
- tobacco mosaic virus
Tobacco mosaic viruse0pathogen - Outcome
- intercellular trafficking via plasmodesmatae0
- Direction
- delayede0
Not reportedTaxon
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TAG4.4 exhibited delayed intercellular trafficking via plasmodesmata of a tobamovirus (tobacco mosaic virus), of a potexvirus (recombinant potato virus X expressing GFP), and of the movement protein (MP) 3a of a cucumovirus (cucumber mosaic virus)”
Claim · model-written summary
TAG4.4 delayed plasmodesmatal trafficking of recombinant potato virus X expressing GFP.
- Material
- TAG4.4e0
- Interactor
- recombinant potato virus X expressing GFPe0pathogen
- Outcome
- intercellular trafficking via plasmodesmatae0
- Direction
- delayede0
Not reportedTaxon
Verbatim
e0“TAG4.4 exhibited delayed intercellular trafficking via plasmodesmata of a tobamovirus (tobacco mosaic virus), of a potexvirus (recombinant potato virus X expressing GFP), and of the movement protein (MP) 3a of a cucumovirus (cucumber mosaic virus)”
Claim · model-written summary
TAG4.4 delayed plasmodesmatal trafficking of cucumber mosaic virus.
- Material
- TAG4.4e0
- Interactor
- cucumber mosaic virus
cucumber mosaic cucumoviruse0pathogen - Outcome
- intercellular trafficking via plasmodesmatae0
- Direction
- delayede0
Not reportedTaxon
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TAG4.4 exhibited delayed intercellular trafficking via plasmodesmata of a tobamovirus (tobacco mosaic virus), of a potexvirus (recombinant potato virus X expressing GFP), and of the movement protein (MP) 3a of a cucumovirus (cucumber mosaic virus)”
Claim · model-written summary
The plasmodesmatal size exclusion limit of TAG4.4 fell from 1.0 to 0.85 nm.
- Material
- TAG4.4e0
- Outcome
- plasmodesmatal size exclusion limit (SEL)e0
- Direction
- reducede0
- Value
- 0.85 nme0
- Qualifier
- from 1.0e0
Not reportedTaxon
Verbatim
e0“the plasmodesmatal size exclusion limit (SEL) of TAG4.4 was also reduced from 1.0 to 0.85 nm”
Claim · model-written summary
Callose deposition increased in TAG4.4 after 32 degrees C treatment.
Not reportedTaxonComparator
Verbatim
e0“Deposition of callose, a substrate for beta-1,3-glucanases, was increased in TAG4.4 in response to 32 degrees C treatment, treatment with the fungal elicitor xylanase, and wounding”
Claim · model-written summary
Callose deposition increased in TAG4.4 after treatment with the fungal elicitor xylanase.
- Material
- TAG4.4e0
- Treatment
- treatment with the fungal elicitor xylanasee0
- Outcome
- Deposition of callosee0
- Direction
- increasede0
Not reportedTaxonComparator
Verbatim
e0“Deposition of callose, a substrate for beta-1,3-glucanases, was increased in TAG4.4 in response to 32 degrees C treatment, treatment with the fungal elicitor xylanase, and wounding”
Claim · model-written summary
Callose deposition increased in TAG4.4 after wounding.
Not reportedTaxonComparator
Verbatim
e0“Deposition of callose, a substrate for beta-1,3-glucanases, was increased in TAG4.4 in response to 32 degrees C treatment, treatment with the fungal elicitor xylanase, and wounding”
Claim · model-written summary
The authors propose that infection induced GLU I may aid viral spread by degrading callose.
Not reportedTaxon
Verbatim
e0“GLU I induction in response to infection may help promote MP-driven virus spread by degrading callose”
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See all 56 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.