Abstract
Strains of the plant-pathogenic bacterium Xanthomonas axonopodis pv. citri are differentiated into two groups with respect to aggressiveness (normal and weak) on Citrus grandis cultivars but not on other Citrus species such as Citrus sinensis. Random mutagenesis using the transposon Tn5 in X. axonopodis pv. citri strain KC21, which showed weak aggressiveness on a C. grandis cultivar, was used to isolate mutant KC21T46, which regained a normal level of aggressiveness on the cultivar. The gene inactivated by the transposon, hssB3.0, was shown to be responsible for the suppression of virulence on C. grandis. Sequence analysis revealed it to be a new member of the pthA homologs, which was almost identical in sequence to the other homologs except for the number of tandem repeats in the central region of the gene. hssB3.0 appears to be a chimera of other pthA homologs, pB3.1 and pB3.7, and could have been generated by recombination between these two genes. Importantly, in X. axonopodis pv. citri, hssB3.0 was found in all of the tested isolates belonging to the weakly aggressive group but not in the isolates of the normally aggressive group. Isolation of the virulence-deficient mutant KC21T14 from KC21, in which the pathogenicity gene pthA-KC21 was disrupted, showed that hssB3.0 induces a defense response on the host but partially interrupts canker development elicited by the pathogenicity gene in this bacterium.
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Claims 10
Claim · model-written summary
Bacterial strains differed in aggressiveness on Citrus grandis but not Citrus sinensis.
- Taxon
- Citrus grandis
Citrus maximae0 - Material
- cultivarse0
- Interactor
- Xanthomonas axonopodis pv. citri
Xanthomonas citrie0pathogen - Outcome
- aggressiveness (normal and weak)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Strains of the plant-pathogenic bacterium Xanthomonas axonopodis pv. citri are differentiated into two groups with respect to aggressiveness (normal and weak) on Citrus grandis cultivars but not on other Citrus species such as Citrus sinensis.”
Claim · model-written summary
Mutant KC21T46 regained normal aggressiveness on a C. grandis cultivar.
Not reportedTaxon
Verbatim
e0“mutant KC21T46, which regained a normal level of aggressiveness on the cultivar.”
Claim · model-written summary
Inactivated hssB3.0 was responsible for suppressed virulence on C. grandis.
- Taxon
- C. grandisCitrus maximae0
- Gene
- hssB3.0e0
- Outcome
- suppression of virulencee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The gene inactivated by the transposon, hssB3.0, was shown to be responsible for the suppression of virulence on C. grandis.”
Claim · model-written summary
Sequence analysis identified hssB3.0 as a new pthA homolog distinguished by tandem repeat number.
Not reportedTaxon
Verbatim
e0“Sequence analysis revealed it to be a new member of the pthA homologs, which was almost identical in sequence to the other homologs except for the number of tandem repeats in the central region of the gene.”
Claim · model-written summary
hssB3.0 appears to be a chimera of pB3.1 and pB3.7.
Not reportedTaxon
Verbatim
e0“hssB3.0 appears to be a chimera of other pthA homologs, pB3.1 and pB3.7”
Claim · model-written summary
hssB3.0 may have arisen through recombination between pB3.1 and pB3.7.
Not reportedTaxonGene
Verbatim
e0“could have been generated by recombination between these two genes.”
Claim · model-written summary
hssB3.0 occurred in every tested weakly aggressive isolate and no normally aggressive isolate.
- Gene
- hssB3.0e0
- Outcome
- found in all of the tested isolates belonging to the weakly aggressive groupe0
- Comparator
- isolates of the normally aggressive groupe0
Not reportedTaxonDirection
Verbatim
e0“hssB3.0 was found in all of the tested isolates belonging to the weakly aggressive group but not in the isolates of the normally aggressive group.”
Claim · model-written summary
The hssB3.0 gene induced a defense response in the host.
Not reportedTaxon
Verbatim
e0“hssB3.0 induces a defense response on the host but partially interrupts canker development elicited by the pathogenicity gene in this bacterium.”
Claim · model-written summary
The hssB3.0 gene partially interrupted canker development.
Not reportedTaxon
Verbatim
e0“hssB3.0 induces a defense response on the host but partially interrupts canker development elicited by the pathogenicity gene in this bacterium.”
Claim · model-written summary
Disruption of pthA-KC21 yielded virulence-deficient mutant KC21T14.
Not reportedTaxon
Verbatim
e0“Isolation of the virulence-deficient mutant KC21T14 from KC21, in which the pathogenicity gene pthA-KC21 was disrupted”