Abstract
Coiled bodies are nuclear organelles that contain components of at least three RNA-processing pathways: pre-mRNA splicing, histone mRNA 3'- maturation, and pre-rRNA processing. Their function remains unknown. However, it has been speculated that coiled bodies may be sites of splicing factor assembly and/or recycling, play a role in histone mRNA 3'-processing, or act as nuclear transport or sorting structures. To study the dynamics of coiled bodies in living cells, we have stably expressed a U2B"-green fluorescent protein fusion in tobacco BY-2 cells and in Arabidopsis plants. Time-lapse confocal microscopy has shown that coiled bodies are mobile organelles in plant cells. We have observed movements of coiled bodies in the nucleolus, in the nucleoplasm, and from the periphery of the nucleus into the nucleolus, which suggests a transport function for coiled bodies. Furthermore, we have observed coalescence of coiled bodies, which suggests a mechanism for the decrease in coiled body number during the cell cycle. Deletion analysis of the U2B" gene construct has shown that the first RNP-80 motif is sufficient for localization to the coiled body.
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Claims 8
Claim · model-written summary
Coiled bodies are mobile in plant cells.
- Taxon
- tobacco
Nicotiana tabacume1 - Organ / stage
- plant cellse0
- Outcome
- coiled bodies are mobile organellese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Time-lapse confocal microscopy has shown that coiled bodies are mobile organelles in plant cells.”
e1“tobacco BY-2 cells and in Arabidopsis plants”
Claim · model-written summary
Coiled bodies move in the nucleolus.
- Taxon
- tobaccoNicotiana tabacume1
- Outcome
- movements of coiled bodies in the nucleoluse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We have observed movements of coiled bodies in the nucleolus, in the nucleoplasm, and from the periphery of the nucleus into the nucleolus”
e1“tobacco BY-2 cells and in Arabidopsis plants”
Claim · model-written summary
Coiled bodies move in the nucleoplasm.
- Taxon
- tobaccoNicotiana tabacume1
- Outcome
- in the nucleoplasme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We have observed movements of coiled bodies in the nucleolus, in the nucleoplasm, and from the periphery of the nucleus into the nucleolus”
e1“tobacco BY-2 cells and in Arabidopsis plants”
Claim · model-written summary
Coiled bodies move from the nuclear periphery into the nucleolus.
- Taxon
- tobaccoNicotiana tabacume1
- Outcome
- from the periphery of the nucleus into the nucleoluse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We have observed movements of coiled bodies in the nucleolus, in the nucleoplasm, and from the periphery of the nucleus into the nucleolus”
e1“tobacco BY-2 cells and in Arabidopsis plants”
Claim · model-written summary
The observed coiled-body movements suggest a transport function.
- Taxon
- tobaccoNicotiana tabacume1
- Outcome
- transport function for coiled bodiese0
- Qualifier
- from the periphery of the nucleus into the nucleoluse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“from the periphery of the nucleus into the nucleolus, which suggests a transport function for coiled bodies.”
e1“tobacco BY-2 cells and in Arabidopsis plants”
Claim · model-written summary
Coiled bodies coalesce.
- Taxon
- tobaccoNicotiana tabacume1
- Outcome
- coalescence of coiled bodiese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we have observed coalescence of coiled bodies”
e1“tobacco BY-2 cells and in Arabidopsis plants”
Claim · model-written summary
Coalescence may explain the decrease in coiled-body number during the cell cycle.
- Taxon
- tobaccoNicotiana tabacume1
- Outcome
- decrease in coiled body number during the cell cyclee0
- Qualifier
- coalescence of coiled bodiese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“coalescence of coiled bodies, which suggests a mechanism for the decrease in coiled body number during the cell cycle.”
e1“tobacco BY-2 cells and in Arabidopsis plants”
Claim · model-written summary
The first RNP-80 motif is sufficient for coiled-body localization.
- Taxon
- tobaccoNicotiana tabacume1
- Treatment
- Deletion analysise0
- Gene
- first RNP-80 motife0
- Outcome
- localization to the coiled bodye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Deletion analysis of the U2B" gene construct has shown that the first RNP-80 motif is sufficient for localization to the coiled body.”
e1“tobacco BY-2 cells and in Arabidopsis plants”