Abstract
Non-nodulating Agrobacterium-like strains identified among root nodule isolates of common bean were labeled with gusA, a reporter gene encoding beta-glucuronidase (GUS). Bean plants were then co-inoculated with an infective Rhizobium strain and labeled transconjugants of Agrobacterium-like strains. Blue staining of nodules showed that Agrobacterium-like strains were able to colonize these symbiotic organs. Isolation and characterization by restriction fragment length polymorphism analysis of PCR-amplified 16S rRNA genes revealed a mixed population of Rhizobium and Agrobacterium-like strains in all nodules showing GUS activity. PCR amplification of the nifH gene and nodulation tests did not show any evidence of acquisition of symbiotic gene by lateral transfer from Rhizobium to Agrobacterium-like strains. Moreover, these strains were able to invade mature nodules. Based on sequencing of the 16S rRNA gene, one of these Agrobacterium-like strains showed 99.4% sequence similarity with Agrobacterium bv. 1 reference strains and 99% similarity with an Agrobacterium bv. 1 strain isolated from Acacia mollisima in Senegal. Agrobacterium tumefaciens C58 and the disarmed variant AT123 did not show any ability to colonize nodules. Co-inoculation of bean seeds with Agrobacterium and Rhizobium strains did not enhance nodulation and plant yield under controlled conditions.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 8
Claim · model-written summary
Agrobacterium-like strains were able to colonize Phaseolus vulgaris nodules.
- Taxon
- Phaseolus vulgaris
Phaseolus vulgarist0 - Organ / stage
- nodulese0
- Interactor
- Agrobacterium-like strainse0symbiont
- Outcome
- colonize these symbiotic organse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Blue staining of nodules showed that Agrobacterium-like strains were able to colonize these symbiotic organs”
t0“Colonization of Phaseolus vulgaris nodules by Agrobacterium-like strains”
Claim · model-written summary
Bean nodules contained a mixed population of Rhizobium and Agrobacterium-like strains.
- Taxon
- Phaseolus vulgarisPhaseolus vulgarist0
- Organ / stage
- nodulese1
- Second interactor
- Agrobacterium-like strainse1symbiont
- Outcome
- mixed populatione1
RhizobiumOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Isolation and characterization by restriction fragment length polymorphism analysis of PCR-amplified 16S rRNA genes revealed a mixed population of Rhizobium and Agrobacterium-like strains in all nodules showing GUS activity”
t0“Colonization of Phaseolus vulgaris nodules by Agrobacterium-like strains”
Claim · model-written summary
No evidence was found for acquisition of the nifH symbiotic gene by Agrobacterium-like strains from Rhizobium by lateral transfer.
- Taxon
- Phaseolus vulgarisPhaseolus vulgarist0
- Interactor
- Agrobacterium-like strainse2symbiont
- Gene
- nifHe2
- Outcome
- acquisition of symbiotic gene by lateral transfere2
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“PCR amplification of the nifH gene and nodulation tests did not show any evidence of acquisition of symbiotic gene by lateral transfer from Rhizobium to Agrobacterium-like strains”
t0“Colonization of Phaseolus vulgaris nodules by Agrobacterium-like strains”
Claim · model-written summary
Agrobacterium-like strains were able to invade mature bean nodules.
- Taxon
- Phaseolus vulgarisPhaseolus vulgarist0
- Organ / stage
- mature nodulese3
- Interactor
- Agrobacterium-like strainse0symbiont
- Outcome
- invade mature nodulese3
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Blue staining of nodules showed that Agrobacterium-like strains were able to colonize these symbiotic organs”
e3“Moreover, these strains were able to invade mature nodules”
t0“Colonization of Phaseolus vulgaris nodules by Agrobacterium-like strains”
Claim · model-written summary
One bean nodule Agrobacterium-like strain showed 99.4% 16S rRNA sequence similarity with Agrobacterium bv. 1 reference strains.
- Taxon
- Phaseolus vulgarisPhaseolus vulgarist0
- Interactor
- Agrobacterium-like strainse4symbiont
- Outcome
- sequence similaritye4
- Value
- 99.4%e4
- Comparator
- Agrobacterium bv. 1 reference strainse4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“one of these Agrobacterium-like strains showed 99.4% sequence similarity with Agrobacterium bv. 1 reference strains”
t0“Colonization of Phaseolus vulgaris nodules by Agrobacterium-like strains”
Claim · model-written summary
A bean nodule Agrobacterium-like strain showed 99% similarity with an Agrobacterium bv. 1 strain isolated from Acacia mollisima.
- Taxon
- Phaseolus vulgarisPhaseolus vulgarist0
- Interactor
- Agrobacterium-like strainse0symbiont
- Outcome
- similaritye5
- Value
- 99%e5
- Comparator
- an Agrobacterium bv. 1 strain isolated from Acacia mollisima in Senegale5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“99% similarity with an Agrobacterium bv. 1 strain isolated from Acacia mollisima in Senegal”
e0“Blue staining of nodules showed that Agrobacterium-like strains were able to colonize these symbiotic organs”
t0“Colonization of Phaseolus vulgaris nodules by Agrobacterium-like strains”
Claim · model-written summary
Agrobacterium tumefaciens C58 and AT123 did not colonize bean nodules.
- Taxon
- Phaseolus vulgarisPhaseolus vulgarist0
- Organ / stage
- nodulese6
- Interactor
- Agrobacterium tumefaciens C58
Agrobacterium tumefaciense6other - Second interactor
- AT123e6other
- Outcome
- ability to colonize nodulese6
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e6“Agrobacterium tumefaciens C58 and the disarmed variant AT123 did not show any ability to colonize nodules”
t0“Colonization of Phaseolus vulgaris nodules by Agrobacterium-like strains”
Claim · model-written summary
Co-inoculation of bean seeds with Agrobacterium and Rhizobium strains did not enhance nodulation or plant yield under controlled conditions.
- Taxon
- Phaseolus vulgarisPhaseolus vulgarist0
- Treatment
- Co-inoculation of bean seeds with Agrobacterium and Rhizobium strainse7
- Interactor
- Agrobacterium
.jpg?width=60)
Agrobacteriume7symbiont - Outcome
- nodulation and plant yielde7
- Direction
- did not enhancee7
- Setting
- controlled conditionse7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e7“Co-inoculation of bean seeds with Agrobacterium and Rhizobium strains did not enhance nodulation and plant yield under controlled conditions”
t0“Colonization of Phaseolus vulgaris nodules by Agrobacterium-like strains”
The same questions in other papers
Rhizobium · symbiosis · Phaseolus vulgaris (common bean)Positive and negative results
1 paper in the same direction as this paper · 16 in the opposite direction
- positive
- positive
- positive
14 papers more in this group
See all 18 papers →inoculation · symbiosis · Phaseolus vulgaris (common bean)Positive and negative results
6 papers in the same direction as this paper · 83 in the opposite direction
- positive
- positive
- positive
86 papers more in this group
See all 90 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.