Abstract
Moringa oleifera Lam. has been used as a traditional medicine for the treatment of numerous diseases. A simultaneous high-performance liquid chromatography (HPLC) analysis was developed and validated for the determination of the contents of crypto-chlorogenic acid, isoquercetin and astragalin, the primary antioxidative compounds, in M. oleifera leaves. HPLC analysis was successfully conducted by using a Hypersil BDS C18 column, eluted with a gradient of methanol-1% acetic acid with a flow rate of 1 mL/min, and detected at 334 nm. Parameters for the validation included linearity, precision, accuracy and limits of detection and quantitation. The developed HPLC method was precise, with relative standard deviation < 2%. The recovery values of crypto-chlorogenic acid, isoquercetin and astragalin in M. oleifera leaf extracts were 98.50, 98.47 and 98.59%, respectively. The average contents of these compounds in the dried ethanolic extracts of the leaves of M. oleifera collected from different regions of Thailand were 0.081, 0.120 and 0.153% (w/w), respectively. The developed HPLC method was appropriate and practical for the simultaneous analysis of crypto-chlorogenic acid, isoquercetin and astragalin in the leaf extract of M. oleifera. This work is valuable as guidance for the standardization of the leaf extracts and pharmaceutical products of M. oleifera.
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Claims 8
Claim · model-written summary
The HPLC method had relative standard deviation below 2%.
- Taxon
- Moringa oleifera Lam.
Moringa oleiferat0 - Outcome
- relative standard deviatione0
- Direction
- precisee0
- Value
- < 2%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The developed HPLC method was precise, with relative standard deviation < 2%.”
t0“Moringa oleifera Lam.”
Claim · model-written summary
Recovery of crypto-chlorogenic acid was 98.50%.
- Taxon
- M. oleiferaMoringa oleiferae0
- Organ / stage
- leaf extractse0
- Outcome
- recovery values of crypto-chlorogenic acide0
- Value
- 98.50e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The recovery values of crypto-chlorogenic acid, isoquercetin and astragalin in M. oleifera leaf extracts were 98.50, 98.47 and 98.59%, respectively.”
Claim · model-written summary
Recovery of isoquercetin was 98.47%.
- Taxon
- M. oleiferaMoringa oleiferae0
- Organ / stage
- leaf extractse0
- Outcome
- recovery values of crypto-chlorogenic acid, isoquercetin and astragaline0
- Value
- 98.47e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The recovery values of crypto-chlorogenic acid, isoquercetin and astragalin in M. oleifera leaf extracts were 98.50, 98.47 and 98.59%, respectively.”
Claim · model-written summary
Recovery of astragalin was 98.59%.
- Taxon
- M. oleiferaMoringa oleiferae0
- Organ / stage
- leaf extractse0
- Outcome
- recovery values of crypto-chlorogenic acid, isoquercetin and astragaline0
- Value
- 98.59%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The recovery values of crypto-chlorogenic acid, isoquercetin and astragalin in M. oleifera leaf extracts were 98.50, 98.47 and 98.59%, respectively.”
Claim · model-written summary
Crypto-chlorogenic acid averaged 0.081% in dried ethanolic Moringa leaf extracts.
- Taxon
- M. oleiferaMoringa oleiferae0
- Organ / stage
- leavese0
- Outcome
- average contents of these compoundse0
- Value
- 0.081e0
- Setting
- Thailande0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The average contents of these compounds in the dried ethanolic extracts of the leaves of M. oleifera collected from different regions of Thailand were 0.081, 0.120 and 0.153% (w/w), respectively.”
Claim · model-written summary
Isoquercetin averaged 0.120% in dried ethanolic Moringa leaf extracts.
- Taxon
- M. oleiferaMoringa oleiferae0
- Organ / stage
- leavese0
- Outcome
- average contents of these compoundse0
- Value
- 0.120e0
- Setting
- Thailande0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The average contents of these compounds in the dried ethanolic extracts of the leaves of M. oleifera collected from different regions of Thailand were 0.081, 0.120 and 0.153% (w/w), respectively.”
Claim · model-written summary
Astragalin averaged 0.153% in dried ethanolic Moringa leaf extracts.
- Taxon
- M. oleiferaMoringa oleiferae0
- Organ / stage
- leavese0
- Outcome
- average contents of these compoundse0
- Value
- 0.153% (w/w)e0
- Setting
- Thailande0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The average contents of these compounds in the dried ethanolic extracts of the leaves of M. oleifera collected from different regions of Thailand were 0.081, 0.120 and 0.153% (w/w), respectively.”
Claim · model-written summary
The HPLC method was judged practical for simultaneous analysis of the three compounds.
- Taxon
- M. oleiferaMoringa oleiferae0
- Outcome
- simultaneous analysis of crypto-chlorogenic acid, isoquercetin and astragaline0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The developed HPLC method was appropriate and practical for the simultaneous analysis of crypto-chlorogenic acid, isoquercetin and astragalin in the leaf extract of M. oleifera.”
t0“Moringa oleifera Lam.”