Abstract
Abstract Calystegines are plant secondary metabolites produced by the roots of a few plant species, and the ability to catabolize calystegines is infrequent in rhizosphere bacteria. In Sinorhizobium meliloti Rm41, the endosymbiont of the legume Medicago sativa , this ability results from the presence of the genes cac (for calystegine catabolism) located on the nonsymbiotic plasmid pRme41a. The effect of the cac catabolic plasmid pRme41a on the ability of Rm41 to colonize the rhizosphere of calystegine‐positive plants was studied using derivatives of Rm41 with or without cac catabolic plasmid. When strains were inoculated alone, the presence of a cac catabolic plasmid had no effect on their colonization of the rhizosphere, regardless of whether plants produced calystegines or not. However, a spontaneous rifampicin‐resistant mutant of Rm41 containing a cac catabolic plasmid reached population levels in the rhizosphere of calystegine‐positive plants that were several orders of magnitude higher than those of the same strain without the plasmid, when each was co‐inoculated with a derivative of Rm41 cured of pRme41a. In contrast, the cac catabolic plasmid provided little or no selective advantage in the rhizosphere of calystegine‐negative plants. In conclusion, the cac catabolic plasmid pRme41a can contribute to the ability of S. meliloti Rm41 to colonize the rhizosphere of alternative, nonlegume plant hosts producing calystegines.
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Claims 4
Claim · model-written summary
When inoculated alone, the plasmid did not affect Rm41 rhizosphere colonization regardless of calystegine production.
Verbatim
e0“When strains were inoculated alone, the presence of a cac catabolic plasmid had no effect on their colonization of the rhizosphere, regardless of whether plants produced calystegines or not.”
Claim · model-written summary
In co-inoculations, plasmid-bearing Rm41 reached rhizosphere levels several orders higher than its plasmid-free counterpart on calystegine-positive plants.
Verbatim
e0“However, a spontaneous rifampicin‐resistant mutant of Rm41 containing a cac catabolic plasmid reached population levels in the rhizosphere of calystegine‐positive plants that were several orders of magnitude higher than those of the same strain without the plasmid, when each was co‐inoculated with a derivative of Rm41 cured of pRme41a.”
Claim · model-written summary
The plasmid conferred little or no selective advantage on calystegine-negative plants.
- Taxon
- plantsViridiplantaee0
- Treatment
- cac catabolic plasmide0
- Interactor
- Sinorhizobium meliloti Rm41
Sinorhizobium meliloti Rm41t0symbiont - Outcome
- selective advantage in the rhizospheree0
- Direction
- little or noe0
- Comparator
- calystegine‐negative plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In contrast, the cac catabolic plasmid provided little or no selective advantage in the rhizosphere of calystegine‐negative plants.”
t0“Sinorhizobium meliloti Rm41”
Claim · model-written summary
The authors concluded that pRme41a can aid S. meliloti Rm41 colonization of calystegine-producing nonlegume hosts.
- Taxon
- plant hostse0
- Interactor
- S. meliloti Rm41
Sinorhizobium melilotie0symbiont - Gene
- cace0
- Outcome
- ability of S. meliloti Rm41 to colonize the rhizospheree0
- Qualifier
- alternative, nonlegume plant hosts producing calysteginese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In conclusion, the cac catabolic plasmid pRme41a can contribute to the ability of S. meliloti Rm41 to colonize the rhizosphere of alternative, nonlegume plant hosts producing calystegines.”