Abstract
Abscisic acid (ABA) regulates plant adaptive responses to various environmental stresses, while L-ascorbic acid (AsA) that is also named vitamin C is an important antioxidant and involves in plant stress tolerance and the immune system in domestic animals. Transgenic tobacco (Nicotiana tabacum L.) and stylo [Stylosanthes guianensis (Aublet) Swartz], a forage legume, plants co-expressing stylo 9-cis-epoxycarotenoid dioxygenase (SgNCED1) and yeast D-arabinono-1,4-lactone oxidase (ALO) genes were generated in this study, and tolerance to drought and chilling was analysed in comparison with transgenic tobacco overexpressing SgNCED1 or ALO and the wild-type plants. Compared to the SgNCED1 or ALO transgenic plants, in which only ABA or AsA levels were increased, both ABA and AsA levels were increased in transgenic tobacco and stylo plants co-expressing SgNCED1 and ALO genes. Compared to the wild type, an enhanced drought tolerance was observed in SgNCED1 transgenic tobacco plants with induced expression of drought-responsive genes, but not in ALO plants, while an enhanced chilling tolerance was observed in ALO transgenic tobaccos with induced expression of cold-responsive genes, but not in SgNCED1 plants. Co-expression of SgNCED1 and ALO genes resulted in elevated tolerance to both drought and chilling in transgenic tobacco and stylo plants with induced expression of both drought and cold-responsive genes. Our result suggests that co-expression of SgNCED1 and ALO genes is an effective way for use in forage plant improvement for increased tolerance to drought and chilling and nutrition quality.
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Claims 9
Claim · model-written summary
Transgenic tobacco co-expressing SgNCED1 and ALO had increased ABA and AsA levels compared with plants carrying SgNCED1 or ALO alone.
- Taxon
- tobacco
Nicotiana tabacume0 - Treatment
- co-expressing SgNCED1 and ALO genese0
- Outcome
- ABA and AsA levelse0
- Direction
- increasede0
- Comparator
- SgNCED1 or ALO transgenic plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Compared to the SgNCED1 or ALO transgenic plants, in which only ABA or AsA levels were increased, both ABA and AsA levels were increased in transgenic tobacco and stylo plants co-expressing SgNCED1 and ALO genes”
Claim · model-written summary
Transgenic stylo co-expressing SgNCED1 and ALO had increased ABA and AsA levels compared with plants carrying SgNCED1 or ALO alone.
- Taxon
- stylo
.jpg?width=60)
Stylosanthes guianensise0 - Treatment
- co-expressing SgNCED1 and ALO genese0
- Outcome
- ABA and AsA levelse0
- Direction
- increasede0
- Comparator
- SgNCED1 or ALO transgenic plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“Compared to the SgNCED1 or ALO transgenic plants, in which only ABA or AsA levels were increased, both ABA and AsA levels were increased in transgenic tobacco and stylo plants co-expressing SgNCED1 and ALO genes”
Claim · model-written summary
SgNCED1 transgenic tobacco showed enhanced drought tolerance with induced expression of drought-responsive genes compared with wild type.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- SgNCED1 transgenic tobacco plantse0
- Outcome
- drought tolerancee0
- Direction
- enhancede0
- Qualifier
- induced expression of drought-responsive genese0
- Comparator
- wild typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Compared to the wild type, an enhanced drought tolerance was observed in SgNCED1 transgenic tobacco plants with induced expression of drought-responsive genes, but not in ALO plants”
Claim · model-written summary
ALO transgenic tobacco did not show enhanced drought tolerance compared with wild type.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- ALO plantse0
- Outcome
- drought tolerancee0
- Direction
- but not in ALO plantse0
- Comparator
- wild typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Compared to the wild type, an enhanced drought tolerance was observed in SgNCED1 transgenic tobacco plants with induced expression of drought-responsive genes, but not in ALO plants”
Claim · model-written summary
ALO transgenic tobacco showed enhanced chilling tolerance with induced expression of cold-responsive genes compared with wild type.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- ALO transgenic tobaccose0
- Outcome
- chilling tolerancee0
- Direction
- enhancede0
- Qualifier
- induced expression of cold-responsive genese0
- Comparator
- wild typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Compared to the wild type, an enhanced drought tolerance was observed in SgNCED1 transgenic tobacco plants with induced expression of drought-responsive genes, but not in ALO plants, while an enhanced chilling tolerance was observed in ALO transgenic tobaccos with induced expression of cold-responsive genes, but not in SgNCED1 plants”
Claim · model-written summary
SgNCED1 transgenic tobacco did not show enhanced chilling tolerance compared with wild type.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- SgNCED1 plantse0
- Outcome
- chilling tolerancee0
- Direction
- but not in SgNCED1 plantse0
- Comparator
- wild typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Compared to the wild type, an enhanced drought tolerance was observed in SgNCED1 transgenic tobacco plants with induced expression of drought-responsive genes, but not in ALO plants, while an enhanced chilling tolerance was observed in ALO transgenic tobaccos with induced expression of cold-responsive genes, but not in SgNCED1 plants”
Claim · model-written summary
Co-expression of SgNCED1 and ALO genes resulted in elevated tolerance to both drought and chilling in transgenic tobacco, with induced expression of both drought and cold-responsive genes.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- Co-expression of SgNCED1 and ALO genese0
- Outcome
- tolerance to both drought and chillinge0
- Direction
- elevatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Co-expression of SgNCED1 and ALO genes resulted in elevated tolerance to both drought and chilling in transgenic tobacco and stylo plants”
Claim · model-written summary
Co-expression of SgNCED1 and ALO genes resulted in elevated tolerance to both drought and chilling in transgenic stylo, with induced expression of both drought and cold-responsive genes.
- Taxon
- styloStylosanthes guianensise0
- Treatment
- Co-expression of SgNCED1 and ALO genese0
- Outcome
- tolerance to both drought and chillinge0
- Direction
- elevatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Co-expression of SgNCED1 and ALO genes resulted in elevated tolerance to both drought and chilling in transgenic tobacco and stylo plants”
Claim · model-written summary
The authors suggest that co-expressing SgNCED1 and ALO is an effective way to improve forage plants for increased drought and chilling tolerance and nutrition quality.
Verbatim
e0“co-expression of SgNCED1 and ALO genes is an effective way for use in forage plant improvement for increased tolerance to drought and chilling and nutrition quality”