Abstract
To overcome the salinity-induced loss of crop yield, a salinity-tolerant trait is required. The SUV3 helicase is involved in the regulation of RNA surveillance and turnover in mitochondria, but the helicase activity of plant SUV3 and its role in abiotic stress tolerance have not been reported so far. Here we report that the Oryza sativa (rice) SUV3 protein exhibits DNA and RNA helicase, and ATPase activities. Furthermore, we report that SUV3 is induced in rice seedlings in response to high levels of salt. Its expression, driven by a constitutive cauliflower mosaic virus 35S promoter in IR64 transgenic rice plants, confers salinity tolerance. The T1 and T2 sense transgenic lines showed tolerance to high salinity and fully matured without any loss in yields. The T2 transgenic lines also showed tolerance to drought stress. These results suggest that the introduced trait is functional and stable in transgenic rice plants. The rice SUV3 sense transgenic lines showed lesser lipid peroxidation, electrolyte leakage and H2 O2 production, along with higher activities of antioxidant enzymes under salinity stress, as compared with wild type, vector control and antisense transgenic lines. These results suggest the existence of an efficient antioxidant defence system to cope with salinity-induced oxidative damage. Overall, this study reports that plant SUV3 exhibits DNA and RNA helicase and ATPase activities, and provides direct evidence of its function in imparting salinity stress tolerance without yield loss. The possible mechanism could be that OsSUV3 helicase functions in salinity stress tolerance by improving photosynthesis and antioxidant machinery in transgenic rice.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 7
Claim · model-written summary
Rice SUV3 protein showed DNA and RNA helicase and ATPase activity.
- Taxon
- Oryza sativa
Oryza sativae0 - Gene
- SUV3e0
- Outcome
- DNA and RNA helicase, and ATPase activitiese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the Oryza sativa (rice) SUV3 protein exhibits DNA and RNA helicase, and ATPase activities.”
Claim · model-written summary
Salt induced SUV3 in rice seedlings.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SUV3 is induced in rice seedlings in response to high levels of salt.”
Claim · model-written summary
SUV3 overexpression conferred salinity tolerance in transgenic IR64 rice.
- Taxon
- riceOryza sativae0
- Material
- IR64 transgenic rice plantse0
- Treatment
- expressione0
- Gene
- SUV3t0
- Outcome
- salinity tolerancee0
- Direction
- conferse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Its expression, driven by a constitutive cauliflower mosaic virus 35S promoter in IR64 transgenic rice plants, confers salinity tolerance.”
t0“OsSUV3 dual helicase functions in salinity stress tolerance by maintaining photosynthesis and antioxidant machinery in rice (Oryza sativa L. cv. IR64)”
Claim · model-written summary
T1 and T2 sense lines matured under high salinity without yield loss.
- Taxon
- riceOryza sativat0
- Material
- T1 and T2 sense transgenic linese0
- Treatment
- high salinitye0
- Outcome
- yieldse0
- Direction
- without any losse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The T1 and T2 sense transgenic lines showed tolerance to high salinity and fully matured without any loss in yields.”
t0“OsSUV3 dual helicase functions in salinity stress tolerance by maintaining photosynthesis and antioxidant machinery in rice (Oryza sativa L. cv. IR64)”
Claim · model-written summary
T2 transgenic rice lines tolerated drought.
- Taxon
- riceOryza sativat0
- Material
- T2 transgenic linese0
- Treatment
- drought stresse0
- Outcome
- tolerancee0
- Direction
- tolerancee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The T2 transgenic lines also showed tolerance to drought stress.”
t0“OsSUV3 dual helicase functions in salinity stress tolerance by maintaining photosynthesis and antioxidant machinery in rice (Oryza sativa L. cv. IR64)”
Claim · model-written summary
SUV3 sense lines had less lipid peroxidation, electrolyte leakage and H2O2 under salinity than controls.
- Taxon
- riceOryza sativae0
- Material
- SUV3 sense transgenic linese0
- Treatment
- salinity stresse0
- Outcome
- lipid peroxidation, electrolyte leakage and H2 O2 productione0
- Direction
- lessere0
- Comparator
- wild type, vector control and antisense transgenic linese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The rice SUV3 sense transgenic lines showed lesser lipid peroxidation, electrolyte leakage and H2 O2 production, along with higher activities of antioxidant enzymes under salinity stress, as compared with wild type, vector control and antisense transgenic lines.”
Claim · model-written summary
SUV3 sense lines had higher antioxidant enzyme activity under salinity than controls.
- Taxon
- riceOryza sativae0
- Material
- SUV3 sense transgenic linese0
- Treatment
- salinity stresse0
- Outcome
- activities of antioxidant enzymese0
- Direction
- highere0
- Comparator
- wild type, vector control and antisense transgenic linese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The rice SUV3 sense transgenic lines showed lesser lipid peroxidation, electrolyte leakage and H2 O2 production, along with higher activities of antioxidant enzymes under salinity stress, as compared with wild type, vector control and antisense transgenic lines.”
The same questions in other papers
salinity · yield · Oryza sativa (rice)Positive and negative results
28 papers in the same direction as this paper · 138 in the opposite direction
- negative
- negative
- negative
163 papers more in this group
See all 167 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.