Abstract
A cDNA clone encoding a putative EIN3-like protein (DC-EIL1) was obtained from total RNA isolated from senescing carnation (Dianthus caryophyllus L.) petals using RT-PCR and RACE techniques. The cDNA (2382 bp) contained an open reading frame of 1986 bp corresponding to 662 amino acids. The amino acid sequence of the N-terminal half of the protein, ranging from 80-300 amino acid residues, had 84% identity with that of the corresponding regions of Arabidopsis EIN3 and tobacco TEIL, although the overall identity was 49% and 52%, respectively. Northern blot analysis revealed that the amount of mRNA corresponding to DC-EIL1 decreased in flower tissues, especially in petals, during natural senescence and senescence induced by exogenously applied ethylene or ABA.
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Claims 6
Claim · model-written summary
A cDNA clone encoding an EIN3-like protein (DC-EIL1) was obtained from senescing carnation petals.
- Taxon
- carnation
Dianthus caryophyllust0 - Organ / stage
- petalse1
- Gene
- DC-EIL1e1
- Outcome
- cDNA clone encoding a putative EIN3-like proteine1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“carnation flower tissues”
e1“A cDNA clone encoding a putative EIN3-like protein (DC-EIL1) was obtained from total RNA isolated from senescing carnation (Dianthus caryophyllus L.) petals using RT-PCR and RACE techniques”
Claim · model-written summary
The N-terminal half of the DC-EIL1 protein had 84% identity with the corresponding regions of Arabidopsis EIN3 and tobacco TEIL.
- Taxon
- carnationDianthus caryophyllust0
- Outcome
- identitye2
- Value
- 84%e2
- Qualifier
- N-terminal halfe2
- Comparator
- Arabidopsis EIN3 and tobacco TEILe2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“carnation flower tissues”
e2“The amino acid sequence of the N-terminal half of the protein, ranging from 80-300 amino acid residues, had 84% identity with that of the corresponding regions of Arabidopsis EIN3 and tobacco TEIL, although the overall identity was 49% and 52%, respectively”
Claim · model-written summary
Overall identity of the DC-EIL1 protein with Arabidopsis EIN3 was 49%.
- Taxon
- carnationDianthus caryophyllust0
- Outcome
- overall identitye2
- Value
- 49%e2
- Comparator
- Arabidopsis EIN3e2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“carnation flower tissues”
e2“The amino acid sequence of the N-terminal half of the protein, ranging from 80-300 amino acid residues, had 84% identity with that of the corresponding regions of Arabidopsis EIN3 and tobacco TEIL, although the overall identity was 49% and 52%, respectively”
Claim · model-written summary
Overall identity of the DC-EIL1 protein with tobacco TEIL was 52%.
- Taxon
- carnationDianthus caryophyllust0
- Outcome
- overall identitye2
- Value
- 52%e2
- Comparator
- tobacco TEILe2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“carnation flower tissues”
e2“The amino acid sequence of the N-terminal half of the protein, ranging from 80-300 amino acid residues, had 84% identity with that of the corresponding regions of Arabidopsis EIN3 and tobacco TEIL, although the overall identity was 49% and 52%, respectively”
Claim · model-written summary
DC-EIL1 mRNA amount decreased in carnation flower tissues, especially petals, during natural senescence.
- Taxon
- carnationDianthus caryophyllust0
- Organ / stage
- petalse3
- Treatment
- natural senescencee3
- Gene
- DC-EIL1e3
- Outcome
- amount of mRNA corresponding to DC-EIL1e3
- Direction
- decreasede3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“carnation flower tissues”
e3“Northern blot analysis revealed that the amount of mRNA corresponding to DC-EIL1 decreased in flower tissues, especially in petals, during natural senescence and senescence induced by exogenously applied ethylene or ABA”
Claim · model-written summary
DC-EIL1 mRNA amount decreased in carnation flower tissues during senescence induced by exogenously applied ethylene or ABA.
- Taxon
- carnationDianthus caryophyllust0
- Organ / stage
- petalse3
- Treatment
- exogenously applied ethylene or ABAe3
- Gene
- DC-EIL1e3
- Outcome
- amount of mRNA corresponding to DC-EIL1e3
- Direction
- decreasede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“carnation flower tissues”
e3“Northern blot analysis revealed that the amount of mRNA corresponding to DC-EIL1 decreased in flower tissues, especially in petals, during natural senescence and senescence induced by exogenously applied ethylene or ABA”