Abstract
Abstract China‐tree or paraiso ( Melia azedarach ) decline, caused by a phytoplasma, is a disease in Argentina for which hitherto no diagnostic reagents were available. The production of a polyclonal antiserum using a purification protocol involving a double Percoll gradient is reported. The antiserum was used successfully in immuno‐dot blot assay to discriminate between infected and healthy plants. It also recognized, although less reliably, other phytoplasmas present in garlic ( Allium sativum ), peach ( Prunus persica ) and tona ( Tona cilliata ). Organisms that by their size and morphology resembled phytoplasmas were detected by immuno‐electron microscopy and immunogold labelling. The ability of the antiserum to discriminate between healthy and infected plants was confirmed by PCR amplification of fragments only from infected plants.
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Claims 6
Claim · model-written summary
Antiserum discriminated infected from healthy China-tree plants.
- Interactor
- Phytoplasma
Candidatus Phytoplasmat0pathogen - Outcome
- discriminate between infected and healthy plantse0
- Comparator
- healthy plantse0
ChinaOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The antiserum was used successfully in immuno‐dot blot assay to discriminate between infected and healthy plants”
t0“Phytoplasma Associated with China‐tree”
Claim · model-written summary
Antiserum also recognized phytoplasmas in garlic.
- Taxon
- garlic
Allium sativume0 - Interactor
- phytoplasmasCandidatus Phytoplasmae0pathogen
- Outcome
- recognized, although less reliably, other phytoplasmase0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“It also recognized, although less reliably, other phytoplasmas present in garlic ( Allium sativum ), peach ( Prunus persica ) and tona ( Tona cilliata )”
Claim · model-written summary
Antiserum also recognized phytoplasmas in peach.
- Taxon
- peach
Prunus persicae0 - Interactor
- phytoplasmasCandidatus Phytoplasmae0pathogen
- Outcome
- recognized, although less reliably, other phytoplasmase0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“It also recognized, although less reliably, other phytoplasmas present in garlic ( Allium sativum ), peach ( Prunus persica ) and tona ( Tona cilliata )”
Claim · model-written summary
Antiserum also recognized phytoplasmas in tona.
- Taxon
- tonae0
- Interactor
- phytoplasmasCandidatus Phytoplasmae0pathogen
- Outcome
- recognized, although less reliably, other phytoplasmase0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“It also recognized, although less reliably, other phytoplasmas present in garlic ( Allium sativum ), peach ( Prunus persica ) and tona ( Tona cilliata )”
Claim · model-written summary
Immunoelectron microscopy and labeling detected phytoplasma-like organisms.
- Interactor
- phytoplasmasCandidatus Phytoplasmae0pathogen
- Outcome
- Organisms that by their size and morphology resembled phytoplasmas were detectede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Organisms that by their size and morphology resembled phytoplasmas were detected by immuno‐electron microscopy and immunogold labelling”
t0“China‐tree”
Claim · model-written summary
PCR confirmed discrimination of healthy and infected plants.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The ability of the antiserum to discriminate between healthy and infected plants was confirmed by PCR amplification of fragments only from infected plants”
t0“China‐tree”