Abstract
Plant and organ culture can readily be applied to the field of medicinal plant and secondary metabolite production. Many studies that have been conducted attest to the utility of in vitro methods for preservation of genetic diversity, propagation of clonal individuals for use in commercial field planting, and production of metabolites directly from culture. Experiments have also shown that tissue culture is an important tool for enhancement of medicinal compound production utilizing various methods including elicitation. This thesis focuses on production of metabolites using an in vitro system. An overview in the form of a literature review is provided, which details the many ways that tissue culture has been applied to medicinal plants. Two major concerns are addressed experimentally, quantity of biomass and concentration of compounds in that biomass. Quantity of biomass was evaluated in a time course factorial experiment employing three different concentrations each of methyl jasmonate (MeJa) and benzyladenine (BA). MeJa negatively impacted biomass accumulation while BA had a positive impact on biomass accumulation. Sugar use was found to directly correlate with dry weight production, and a system for long-term growth of plant materials was developed. Concentrations of compounds in the biomass were explored by observing effects of media additions of proline, a natural source of proline (Gropro), MeJa, phenylalanine, chitosan, and nitrogen stress. MeJa and phenylalanine were utilized in a factorial experiment, and both compounds reduced biomass and secondary metabolite production. Proline, Gropro, MeJa, chitosan, and nitrogen stress were evaluated in comparison with a control in a separate experiment. Only one treatment, nitrogen stress, was successful in upregulating
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Claims 6
Claim · model-written summary
MeJa negatively impacted biomass accumulation of turmeric in vitro.
- Taxon
- Curcuma longa L.
_W_IMG_2440.jpg?width=60)
Curcuma longat0 - Treatment
- MeJae2
- Outcome
- biomass accumulatione2
- Direction
- negatively impactede2
- Setting
- in vitro systeme6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_W_IMG_2440.jpg?width=500)
Verbatim
t0“Development of in vitro protocols to enhance secondary metabolite production from turmeric (Curcuma longa L.)”
e1“employing three different concentrations each of methyl jasmonate (MeJa) and benzyladenine (BA)”
e2“MeJa negatively impacted biomass accumulation while BA had a positive impact on biomass accumulation”
e6“This thesis focuses on production of metabolites using an in vitro system”
Claim · model-written summary
BA had a positive impact on biomass accumulation of turmeric in vitro.
- Taxon
- Curcuma longa L.Curcuma longat0
- Treatment
- benzyladenine (BA)e1
- Outcome
- biomass accumulatione2
- Direction
- positive impacte2
- Setting
- in vitro systeme6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Development of in vitro protocols to enhance secondary metabolite production from turmeric (Curcuma longa L.)”
e1“employing three different concentrations each of methyl jasmonate (MeJa) and benzyladenine (BA)”
e2“MeJa negatively impacted biomass accumulation while BA had a positive impact on biomass accumulation”
e6“This thesis focuses on production of metabolites using an in vitro system”
Claim · model-written summary
Sugar use directly correlated with dry weight production in turmeric cultures.
- Taxon
- Curcuma longa L.Curcuma longat0
- Compound
- Sugar usee3
- Outcome
- dry weight productione3
- Direction
- directly correlatee3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Development of in vitro protocols to enhance secondary metabolite production from turmeric (Curcuma longa L.)”
e3“Sugar use was found to directly correlate with dry weight production”
Claim · model-written summary
A system for long-term growth of turmeric plant materials was developed.
- Taxon
- Curcuma longa L.Curcuma longat0
- Outcome
- a system for long-term growth of plant materialse4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Schema note from the model: method development with no comparator or value; recorded as observation
Verbatim
t0“Development of in vitro protocols to enhance secondary metabolite production from turmeric (Curcuma longa L.)”
e4“a system for long-term growth of plant materials was developed”
Claim · model-written summary
MeJa reduced biomass and secondary metabolite production of turmeric.
- Taxon
- Curcuma longa L.Curcuma longat0
- Treatment
- MeJae5
- Outcome
- biomass and secondary metabolite productione5
- Direction
- reducede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Development of in vitro protocols to enhance secondary metabolite production from turmeric (Curcuma longa L.)”
e5“MeJa and phenylalanine were utilized in a factorial experiment, and both compounds reduced biomass and secondary metabolite production”
Claim · model-written summary
Phenylalanine reduced biomass and secondary metabolite production of turmeric.
- Taxon
- Curcuma longa L.Curcuma longat0
- Treatment
- phenylalaninee5
- Outcome
- biomass and secondary metabolite productione5
- Direction
- reducede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Development of in vitro protocols to enhance secondary metabolite production from turmeric (Curcuma longa L.)”
e5“MeJa and phenylalanine were utilized in a factorial experiment, and both compounds reduced biomass and secondary metabolite production”
The same questions in other papers
N-benzyladenine · growth · Curcuma longa (turmeric)Results agree
7 papers in the same direction as this paper
- positive
- positive
- positive
4 papers more in this group
See all 8 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.