Abstract
Resistance to flax rust Melampsora lini (Ehrenb.) Lév. in flax carrying the N resistance gene is determined by 24 h postinoculation, at which time hypersensitivity is observed. We have examined protein synthesis in cotyledons inoculated with both virulent and avirulent races of rust by in vivo labelling with [ 35 S]methionine. The pattern of protein synthesis was assessed by one- and two-dimensional electrophoresis 8, 13, and 18 h after inoculation. No changes in protein synthesis were observed in the first 14 h following inoculation; however, by 18 h after inoculation the susceptible combination showed a marked decrease in protein synthesis (22%; P = 0.01). This could be largely accounted for by the reduced synthesis of the ribulose 1,5-bisphosphate carboxylase large subunit, which was readily quantified on electrophoresis gels. In addition, a 30-kDa polypeptide also declined in the susceptible combination. Two-dimensional electrophoresis enabled changes to be detected in the synthesis of other minor polypeptides. None of these changes were observed in the resistant combination in which a small increase in the synthesis of the ribulose 1,5-bisphosphate carboxylase large subunit and the 30-kDa polypeptide was found. These results indicate that the outcome of the host–pathogen interaction has already been determined by 18 h after inoculation.
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Claims 7
Claim · model-written summary
No protein synthesis change was observed in the first 14 hours after inoculation.
Not reportedTaxonOrgan / stage
Verbatim
e0“No changes in protein synthesis were observed in the first 14 h following inoculation”
Claim · model-written summary
The susceptible combination showed a 22% decrease in protein synthesis by 18 hours after inoculation.
Not reportedTaxon
Verbatim
e0“by 18 h after inoculation the susceptible combination showed a marked decrease in protein synthesis (22%; P = 0.01).”
Claim · model-written summary
Synthesis of ribulose 1,5-bisphosphate carboxylase large subunit was reduced in the susceptible combination.
- Gene
- ribulose 1,5-bisphosphate carboxylase large subunite0
- Outcome
- synthesis of the ribulose 1,5-bisphosphate carboxylase large subunite0
- Direction
- reducede0
Not reportedTaxon
Verbatim
e0“This could be largely accounted for by the reduced synthesis of the ribulose 1,5-bisphosphate carboxylase large subunit”
Claim · model-written summary
A 30-kDa polypeptide declined in the susceptible combination.
Not reportedTaxon
Verbatim
e0“a 30-kDa polypeptide also declined in the susceptible combination.”
Claim · model-written summary
Synthesis changes in other minor polypeptides were detected.
- Outcome
- changes to be detected in the synthesis of other minor polypeptidese0
Not reportedTaxon
Verbatim
e0“Two-dimensional electrophoresis enabled changes to be detected in the synthesis of other minor polypeptides.”
Claim · model-written summary
The resistant combination did not show the susceptible pattern of changes.
Not reportedTaxon
Verbatim
e0“None of these changes were observed in the resistant combination”
Claim · model-written summary
Ribulose 1,5-bisphosphate carboxylase large subunit and 30-kDa polypeptide synthesis increased slightly in the resistant combination.
- Outcome
- synthesis of the ribulose 1,5-bisphosphate carboxylase large subunit and the 30-kDa polypeptidee0
- Direction
- increasee0
- Qualifier
- smalle0
Not reportedTaxon
Verbatim
e0“in which a small increase in the synthesis of the ribulose 1,5-bisphosphate carboxylase large subunit and the 30-kDa polypeptide was found.”