Abstract
The Rp1-D gene for resistance to maize common rust (Puccinia sorghi) is a member of a complex locus (haplotype) composed of Rp1-D and approximately eight other gene homologs. The identity of Rp1-D was demonstrated by using two independent gene-tagging approaches with the transposons Mutator and Dissociation. PIC20, a disease resistance (R) gene analog probe previously mapped to the rp1 locus, detected insertion of Dissociation in an Rp1-D mutation and excision in three revertants. Independent libraries probed with the PIC20 or Mutator probes resulted in isolation of the same gene sequence. Rp1-D belongs to the nucleotide binding site, leucine-rich repeat class of R genes. However, unlike the rust resistance genes M and L6 from flax, the maize Rp1-D gene does not encode an N-terminal domain with similarity to the signal transduction domains of the Drosophila Toll protein and mammalian interleukin-1 receptor. Although the abundance of transcripts of genes from the rp1 complex changed with leaf age, there was no evidence of any change due to inoculation with avirulent or virulent rust biotypes. A set of 27 Rp1-D mutants displayed at least nine different deletions of Rp1-D gene family members that were consistent with unequal crossing-over events. One mutation (Rp1-D*-24) resulted in deletion of all but one gene family member. Other unique deletions were observed in the disease lesion mimic Rp1-D*-21 and the partially susceptible mutant Rp1-D*-5. Different rp1 specificities have distinct DNA fingerprints (haplotypes). Analysis of recombinants between rp1 specificities indicated that recombination had occurred within the rp1 gene complex. Similar analyses indicated that the rust R genes at the rp5 locus, 2 centimorgans distal to rp1, are not closely related to Rp1-D.
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Claims 14
Claim · model-written summary
Rp1-D belongs to a complex locus with approximately eight other homologs.
- Interactor
- Puccinia sorghi
Puccinia sorghie0pathogen - Gene
- Rp1-De0
- Outcome
- complex locus (haplotype)e0
- Value
- approximately eight other gene homologse0
Zea maysOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The Rp1-D gene for resistance to maize common rust (Puccinia sorghi) is a member of a complex locus (haplotype) composed of Rp1-D and approximately eight other gene homologs.”
Claim · model-written summary
Two independent gene tagging approaches established the identity of Rp1-D.
Verbatim
e0“The identity of Rp1-D was demonstrated by using two independent gene-tagging approaches with the transposons Mutator and Dissociation.”
t0“Maize Rp1-D Rust Resistance Haplotype”
Claim · model-written summary
PIC20 detected Dissociation insertion in an Rp1-D mutant and excision in three revertants.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PIC20, a disease resistance (R) gene analog probe previously mapped to the rp1 locus, detected insertion of Dissociation in an Rp1-D mutation and excision in three revertants.”
t0“Maize Rp1-D Rust Resistance Haplotype”
Claim · model-written summary
Independent PIC20- and Mutator-probed libraries yielded the same gene sequence.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Independent libraries probed with the PIC20 or Mutator probes resulted in isolation of the same gene sequence.”
t0“Maize Rp1-D Rust Resistance Haplotype”
Claim · model-written summary
Rp1-D is a nucleotide binding site, leucine-rich repeat resistance gene.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Rp1-D belongs to the nucleotide binding site, leucine-rich repeat class of R genes.”
t0“Maize Rp1-D Rust Resistance Haplotype”
Claim · model-written summary
Unlike flax M and L6, maize Rp1-D lacks an N-terminal domain similar to Toll and interleukin-1 receptor signaling domains.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“unlike the rust resistance genes M and L6 from flax, the maize Rp1-D gene does not encode an N-terminal domain with similarity to the signal transduction domains of the Drosophila Toll protein and mammalian interleukin-1 receptor.”
Claim · model-written summary
Transcripts of genes in the rp1 complex changed with leaf age.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the abundance of transcripts of genes from the rp1 complex changed with leaf age”
t0“Maize Rp1-D Rust Resistance Haplotype”
Claim · model-written summary
Inoculation with avirulent or virulent rust biotypes showed no evidence of changing rp1-complex transcript abundance.
- Treatment
- inoculation with avirulent or virulent rust biotypese0
- Outcome
- abundance of transcriptse0
- Direction
- no evidence of any changee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Although the abundance of transcripts of genes from the rp1 complex changed with leaf age, there was no evidence of any change due to inoculation with avirulent or virulent rust biotypes.”
t0“Maize Rp1-D Rust Resistance Haplotype”
Claim · model-written summary
Twenty-seven Rp1-D mutants displayed at least nine distinct deletions consistent with unequal crossing over.
- Material
- Rp1-D mutantse0
- Gene
- Rp1-De0
- Outcome
- deletions of Rp1-D gene family memberse0
- Value
- at least nine different deletionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A set of 27 Rp1-D mutants displayed at least nine different deletions of Rp1-D gene family members that were consistent with unequal crossing-over events.”
t0“Maize Rp1-D Rust Resistance Haplotype”
Claim · model-written summary
Rp1-D*-24 deleted all but one gene family member.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“One mutation (Rp1-D*-24) resulted in deletion of all but one gene family member.”
t0“Maize Rp1-D Rust Resistance Haplotype”
Claim · model-written summary
Distinct deletions occurred in lesion-mimic Rp1-D*-21 and partially susceptible Rp1-D*-5.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Other unique deletions were observed in the disease lesion mimic Rp1-D*-21 and the partially susceptible mutant Rp1-D*-5.”
t0“Maize Rp1-D Rust Resistance Haplotype”
Claim · model-written summary
Different rp1 resistance specificities have distinct DNA fingerprints.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Different rp1 specificities have distinct DNA fingerprints (haplotypes).”
t0“Maize Rp1-D Rust Resistance Haplotype”
Claim · model-written summary
Recombination occurred within the rp1 gene complex.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Analysis of recombinants between rp1 specificities indicated that recombination had occurred within the rp1 gene complex.”
t0“Maize Rp1-D Rust Resistance Haplotype”
Claim · model-written summary
Rust resistance genes at rp5 were not closely related to Rp1-D.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the rust R genes at the rp5 locus, 2 centimorgans distal to rp1, are not closely related to Rp1-D.”
t0“Maize Rp1-D Rust Resistance Haplotype”
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