Abstract
Pathogenesis-related protein-1a (PR-1a) is a protein of unknown function that is strongly induced during the onset of systemic acquired resistance (SAR) in tobacco. The expression of PR-1a is under complex regulation that is controlled at least partially by the rate of transcription. In this study, we demonstrated that 661 bp of 5' flanking DNA was sufficient to impart tobacco mosaic virus and salicylic acid inducibility to a reporter gene. The PR-1a promoter did not respond significantly to treatments with either auxin or cytokinin. Experiments with the protein synthesis inhibitor cycloheximide indicated that protein synthesis is required for salicylate-dependent mRNA accumulation. At flowering, the PR-1a gene was expressed primarily in the mesophyll and epidermal tissues of the leaf blade and the sepals of the flower. Several artifacts, most importantly ectopic expression in pollen, were associated with the use of the beta-glucuronidase reporter gene.
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Claims 7
Claim · model-written summary
A 661 bp flanking region conferred tobacco mosaic virus inducibility to a reporter gene.
- Taxon
- tobacco
Nicotiana tabacumt0 - Treatment
- tobacco mosaic viruse0
- Interactor
- tobacco mosaic virus
Tobacco mosaic viruse0pathogen - Gene
- reporter genee0
- Outcome
- inducibility to a reporter genee0
- Value
- 661 bpe0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“661 bp of 5' flanking DNA was sufficient to impart tobacco mosaic virus and salicylic acid inducibility to a reporter gene”
t0“tobacco”
Claim · model-written summary
The same 661 bp region conferred salicylic acid inducibility to a reporter gene.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- salicylic acide0
- Outcome
- inducibility to a reporter genee0
- Value
- 661 bpe0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“661 bp of 5' flanking DNA was sufficient to impart tobacco mosaic virus and salicylic acid inducibility to a reporter gene”
t0“tobacco”
Claim · model-written summary
The PR-1a promoter did not respond significantly to auxin.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- auxine0
- Gene
- PR-1ae0
- Outcome
- PR-1a promotere0
- Direction
- did not responde0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The PR-1a promoter did not respond significantly to treatments with either auxin or cytokinin”
t0“tobacco”
Claim · model-written summary
The PR-1a promoter did not respond significantly to cytokinin.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- cytokinine0
- Gene
- PR-1ae0
- Outcome
- PR-1a promotere0
- Direction
- did not responde0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The PR-1a promoter did not respond significantly to treatments with either auxin or cytokinin”
t0“tobacco”
Claim · model-written summary
Protein synthesis was required for salicylate-dependent mRNA accumulation.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- cycloheximidee0
- Outcome
- salicylate-dependent mRNA accumulatione0
- Qualifier
- protein synthesis is requirede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Experiments with the protein synthesis inhibitor cycloheximide indicated that protein synthesis is required for salicylate-dependent mRNA accumulation”
t0“tobacco”
Claim · model-written summary
At flowering, PR-1a expression was primarily in leaf mesophyll and epidermis and flower sepals.
- Taxon
- tobaccoNicotiana tabacumt0
- Organ / stage
- mesophyll and epidermal tissues of the leaf blade and the sepals of the flowere0
- Gene
- PR-1ae0
- Outcome
- was expressed primarilye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“At flowering, the PR-1a gene was expressed primarily in the mesophyll and epidermal tissues of the leaf blade and the sepals of the flower”
t0“tobacco”
Claim · model-written summary
The beta-glucuronidase reporter gene produced artifacts including ectopic pollen expression.
- Taxon
- tobaccoNicotiana tabacumt0
- Organ / stage
- pollene0
- Gene
- beta-glucuronidase reporter genee0
- Outcome
- ectopic expression in pollene0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Several artifacts, most importantly ectopic expression in pollen, were associated with the use of the beta-glucuronidase reporter gene”
t0“tobacco”
The same questions in other papers
salicylic acid · molecular: expression · Nicotiana tabacum (tobacco)Positive and negative results
24 papers in the same direction as this paper · 11 in the opposite direction
- no effect
- positive
- positive
32 papers more in this group
See all 36 papers →class · quality: protein · Nicotiana tabacum (tobacco)Positive and negative results
2 papers in the same direction as this paper · 3 in the opposite direction
- positive
- positive
- positive
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.