Abstract
Axenic cultures of the thalloid liverwort Cryptomitrium himalayense Kashyap were established from spores and propagated in vitro under a variety of culture media and controlled environments to determine the optimum conditions for the onset of sexual phase. Enhanced vegetative growth in form of overlapping rosette-forming thalli occurs on half strength Knop's medium as well as in Hoagland medium under continuous illumination of 2.93 W/m2 at 21°C from the production of innovations from the basal parts of the dorsal surfaces of the thalli. Thalli became shorter and produced tuberous swelling following nutrient depletion and drying out of the medium. Sex organs were produced only on Hoagland medium supplemented with 1% sucrose under a long day regime with colder nights (16 hours light at 21°C and of 8 hours darkness at 15°C). Thalli acclimatized and transferred on soil also produced sex organs under similar conditions of photoperiod and temperature.
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Claims 5
Claim · model-written summary
Vegetative growth of Cryptomitrium himalayense was enhanced on half strength Knop's medium and on Hoagland medium under continuous illumination at 21°C.
- Taxon
- Cryptomitrium himalayenseCryptomitrium himalayenset0
- Treatment
- half strength Knop's mediume0
- Outcome
- vegetative growthe0
- Direction
- Enhancede0
- Qualifier
- Hoagland mediume0
- Setting
- in vitroe5
Not reportedComparator
Verbatim
e0“Enhanced vegetative growth in form of overlapping rosette-forming thalli occurs on half strength Knop's medium as well as in Hoagland medium”
e5“propagated in vitro under a variety of culture media and controlled environments”
t0“Cryptomitrium himalayense”
Claim · model-written summary
Thalli became shorter following nutrient depletion and drying out of the medium.
- Taxon
- Cryptomitrium himalayenseCryptomitrium himalayenset0
- Treatment
- nutrient depletion and drying out of the mediume1
- Outcome
- Thallie1
- Direction
- became shortere1
Not reportedComparator
Verbatim
e1“Thalli became shorter and produced tuberous swelling following nutrient depletion and drying out of the medium”
t0“Cryptomitrium himalayense”
Claim · model-written summary
Thalli produced tuberous swelling following nutrient depletion and drying out of the medium.
- Taxon
- Cryptomitrium himalayenseCryptomitrium himalayenset0
- Treatment
- nutrient depletion and drying out of the mediume1
- Outcome
- tuberous swellinge1
Not reportedComparatorDirection
Verbatim
e1“Thalli became shorter and produced tuberous swelling following nutrient depletion and drying out of the medium”
t0“Cryptomitrium himalayense”
Claim · model-written summary
Sex organs were produced only on Hoagland medium supplemented with 1% sucrose under a long day regime with colder nights.
- Taxon
- Cryptomitrium himalayenseCryptomitrium himalayenset0
- Treatment
- Hoagland medium supplemented with 1% sucrosee2
- Outcome
- Sex organse2
- Qualifier
- under a long day regime with colder nightse2
Not reportedComparatorDirection
Verbatim
e2“Sex organs were produced only on Hoagland medium supplemented with 1% sucrose under a long day regime with colder nights (16 hours light at 21°C and of 8 hours darkness at 15°C)”
t0“Cryptomitrium himalayense”
Claim · model-written summary
Thalli acclimatized and transferred on soil also produced sex organs under similar photoperiod and temperature conditions.
- Taxon
- Cryptomitrium himalayenseCryptomitrium himalayenset0
- Treatment
- acclimatized and transferred on soile3
- Outcome
- sex organse3
Not reportedComparatorDirection
Verbatim
e3“Thalli acclimatized and transferred on soil also produced sex organs under similar conditions of photoperiod and temperature”
t0“Cryptomitrium himalayense”