Abstract
Alternative oxidase (Aox) is a nuclear-encoded mitochondrial protein. In soybean (Glycine max), the three members of the gene family have been shown to be differentially expressed during normal plant development and in response to stresses. To examine the function of the Aox promoters, genomic fragments were obtained for all three soybean genes: Aox1, Aox2a, and Aox2b. The regions of these fragments immediately upstream of the coding regions were used to drive beta-glucuronidase (GUS) expression during transient transformation of soybean suspension culture cells and stable transformation of Arabidopsis. The expression patterns of the GUS reporter genes in soybean cells were in agreement with the presence or absence of the various endogenous Aox proteins, determined by immunoblotting. Deletion of different portions of the upstream regions identified sequences responsible for both positive and negative regulation of Aox gene expression in soybean cells. Reporter gene analysis in Arabidopsis plants showed differential tissue expression patterns driven by the three upstream regions, similar to those reported for the endogenous proteins in soybean. The expression profiles of all five members of the Arabidopsis Aox gene family were examined also, to compare with GUS expression driven by the soybean upstream fragments. Even though the promoter activity of the upstream fragments from soybean Aox2a and Aox2b displayed the same tissue specificity in Arabidopsis as they do in soybean, the most prominently expressed endogenous genes in all tissues of Arabidopsis were of the Aox1 type. Thus although regulation of Aox expression generally appears to involve the same signals in different species, different orthologs of Aox may respond variously to these signals. A comparison of upstream sequences between soybean Aox genes and similarly expressed Arabidopsis Aox genes identified common motifs.
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Claims 7
Claim · model-written summary
GUS expression driven by the soybean Aox promoters agreed with the presence or absence of endogenous Aox proteins in soybean cells.
- Taxon
- soybean
Glycine maxe0 - Gene
- Aoxe1
- Outcome
- expression patterns of the GUS reporter genese1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In soybean (Glycine max), the three members of the gene family have been shown to be differentially expressed during normal plant development”
e1“The expression patterns of the GUS reporter genes in soybean cells were in agreement with the presence or absence of the various endogenous Aox proteins”
Claim · model-written summary
Deletion of portions of the soybean Aox upstream regions identified sequences responsible for both positive and negative regulation of Aox expression.
- Taxon
- soybeanGlycine maxe0
- Treatment
- Deletion of different portions of the upstream regionse2
- Outcome
- Aox gene expressione2
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In soybean (Glycine max), the three members of the gene family have been shown to be differentially expressed during normal plant development”
e2“Deletion of different portions of the upstream regions identified sequences responsible for both positive and negative regulation of Aox gene expression in soybean cells”
Claim · model-written summary
Soybean Aox upstream regions drove differential tissue expression patterns in Arabidopsis.
- Taxon
- Arabidopsis
Arabidopsise3 - Outcome
- differential tissue expression patternse3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“Reporter gene analysis in Arabidopsis plants showed differential tissue expression patterns driven by the three upstream regions”
Claim · model-written summary
Promoter fragments from soybean Aox2a and Aox2b showed in Arabidopsis the same tissue specificity as in soybean.
- Taxon
- ArabidopsisArabidopsise4
- Gene
- Aox2a and Aox2be4
- Outcome
- promoter activitye4
- Qualifier
- same tissue specificitye4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“Even though the promoter activity of the upstream fragments from soybean Aox2a and Aox2b displayed the same tissue specificity in Arabidopsis as they do in soybean”
Claim · model-written summary
The most prominently expressed endogenous Arabidopsis genes in all tissues were of the Aox1 type.
- Taxon
- ArabidopsisArabidopsise5
- Gene
- Aox1 typee5
- Outcome
- most prominently expressed endogenous genese5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“the most prominently expressed endogenous genes in all tissues of Arabidopsis were of the Aox1 type”
Claim · model-written summary
Different soybean Aox orthologs may respond differently to the same regulatory signals.
- Taxon
- soybeanGlycine maxe0
- Gene
- Aoxe6
- Outcome
- respond variously to these signalse6
- Qualifier
- different orthologs of Aoxe6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In soybean (Glycine max), the three members of the gene family have been shown to be differentially expressed during normal plant development”
e6“different orthologs of Aox may respond variously to these signals”
Claim · model-written summary
Comparing upstream sequences of soybean Aox genes with Arabidopsis Aox genes identified common motifs.
- Taxon
- soybeanGlycine maxe7
- Material
- upstream sequencese7
- Outcome
- common motifse7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e7“A comparison of upstream sequences between soybean Aox genes and similarly expressed Arabidopsis Aox genes identified common motifs”