Abstract
Microtubule arrays in living cells were analysed during Arabidopsis stomatal development in order to more closely define stages in the pathway and contexts where intercellular signalling might operate. Arabidopsis stomata are patterned iteratively via the orientation of an asymmetric division in a cell located next to an existing stoma. It was found that preprophase bands of microtubules (PPBs) were correctly placed away from stomata and from two types of precursor cells. This suggests that all three cell types participate in an intercellular signalling pathway that orients the division site. These and other asymmetric divisions in the pathway were preceded by a polarized cytoplasm, with the PPB around the nucleus at one end, and the vacuole at the other. PPBs before symmetric divisions of guard mother cells (GMCs) were broader than those in asymmetric divisions, and the GMC division site was marked by unusual end-wall thickenings. This work identifies an accessible system for studying cytoskeletal function and provides a foundation for analysing the role of genes involved in stomatal development.
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Claims 5
Claim · model-written summary
Preprophase bands were placed away from stomata and two precursor cell types.
- Taxon
- Arabidopsis
Arabidopsist0 - Organ / stage
- stomatae0
- Outcome
- preprophase bands of microtubules (PPBs) were correctly placed away from stomata and from two types of precursor cellse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“It was found that preprophase bands of microtubules (PPBs) were correctly placed away from stomata and from two types of precursor cells.”
t0“Arabidopsis”
Claim · model-written summary
Three cell types may signal to orient the division site.
- Taxon
- ArabidopsisArabidopsist0
- Outcome
- orients the division sitee0
- Qualifier
- intercellular signalling pathwaye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This suggests that all three cell types participate in an intercellular signalling pathway that orients the division site.”
t0“Arabidopsis”
Claim · model-written summary
Asymmetric divisions were preceded by polarized cytoplasm with the band and vacuole at opposite ends.
- Taxon
- ArabidopsisArabidopsist0
- Outcome
- polarized cytoplasme0
- Qualifier
- PPB around the nucleus at one end, and the vacuole at the othere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These and other asymmetric divisions in the pathway were preceded by a polarized cytoplasm, with the PPB around the nucleus at one end, and the vacuole at the other.”
t0“Arabidopsis”
Claim · model-written summary
Preprophase bands before symmetric guard mother cell divisions were broader than in asymmetric divisions.
- Taxon
- ArabidopsisArabidopsist0
- Organ / stage
- guard mother cells (GMCs)e0
- Outcome
- PPBse0
- Direction
- broadere0
- Comparator
- asymmetric divisionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PPBs before symmetric divisions of guard mother cells (GMCs) were broader than those in asymmetric divisions”
t0“Arabidopsis”
Claim · model-written summary
Guard mother cell division sites had unusual end-wall thickenings.
- Taxon
- ArabidopsisArabidopsist0
- Organ / stage
- GMCe0
- Outcome
- unusual end-wall thickeningse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the GMC division site was marked by unusual end-wall thickenings.”
t0“Arabidopsis”