Abstract
ng from delayed separation of chromatids and typical bridges were observed in Feulgen preparations. The analysis of C-banded anaphases showed that delayed chromatids were held together at heterochromatic knob sites (primary event), and the presence of typical bridges with and without bands corresponding to knobs. These events suggest the occurrence of breakage-fusion-bridge (BFB) cycles initiated by chromosome arms broken during the primary event. Additional evidence for such a mechanism was the presence of gross aberrations involving chromosome 7, detected in several C-banded metaphases of some cultures. It is hypothesized that such aberrations are duplication deficiencies produced by BFB cycles and chromosome healing that would have occurred after some cell divisions.
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Claims 6
Claim · model-written summary
Delayed chromatid separation and typical bridges were observed in Feulgen preparations of maize callus.
Zea maysNot reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Chromosome Breakage in Maize Callus Culture”
e1“ng from delayed separation of chromatids and typical bridges were observed in Feulgen preparations”
Claim · model-written summary
Delayed chromatids were held together at heterochromatic knob sites, the primary event, in C-banded anaphases.
- Organ / stage
- C-banded anaphasese2
- Outcome
- delayed chromatids were held together at heterochromatic knob sitese2
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Chromosome Breakage in Maize Callus Culture”
e2“The analysis of C-banded anaphases showed that delayed chromatids were held together at heterochromatic knob sites (primary event)”
Claim · model-written summary
Typical bridges with and without bands corresponding to knobs were present in the analysed cells.
- Outcome
- typical bridges with and without bands corresponding to knobse3
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Chromosome Breakage in Maize Callus Culture”
e3“the presence of typical bridges with and without bands corresponding to knobs”
Claim · model-written summary
The observations suggest breakage-fusion-bridge cycles initiated by chromosome arms broken during the primary event.
- Outcome
- occurrence of breakage-fusion-bridge (BFB) cyclese4
- Qualifier
- chromosome arms broken during the primary evente4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Chromosome Breakage in Maize Callus Culture”
e4“These events suggest the occurrence of breakage-fusion-bridge (BFB) cycles initiated by chromosome arms broken during the primary event”
Claim · model-written summary
Gross aberrations involving chromosome 7 were detected in several C-banded metaphases of some cultures.
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Chromosome Breakage in Maize Callus Culture”
e5“gross aberrations involving chromosome 7, detected in several C-banded metaphases of some cultures”
Claim · model-written summary
The aberrations are hypothesized to be duplication deficiencies produced by BFB cycles and chromosome healing.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Chromosome Breakage in Maize Callus Culture”
e6“It is hypothesized that such aberrations are duplication deficiencies produced by BFB cycles and chromosome healing that would have occurred after some cell divisions”