Abstract
The Arabidopsis NPR1 gene is a positive regulator of inducible plant disease resistance. Expression of NPR1 is induced by pathogen infection or treatment with defense-inducing compounds such as salicylic acid (SA). Transgenic plants overexpressing NPR1 exhibit enhanced resistance to a broad spectrum of microbial pathogens, whereas plants underexpressing the gene are more susceptible to pathogen infection. These results suggest that regulation of NPR1 gene expression is important for the activation of plant defense responses. In the present study, we report the identification of W-box sequences in the promoter region of the NPR1 gene that are recognized specifically by SA-induced WRKY DNA binding proteins from Arabidopsis. Mutations in these W-box sequences abolished their recognition by WRKY DNA binding proteins, rendered the promoter unable to activate a downstream reporter gene, and compromised the ability of NPR1 to complement npr1 mutants for SA-induced defense gene expression and disease resistance. These results provide strong evidence that certain WRKY genes act upstream of NPR1 and positively regulate its expression during the activation of plant defense responses. Consistent with this model, we found that SA-induced expression of a number of WRKY genes was independent of NPR1.
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Claims 6
Claim · model-written summary
SA-induced Arabidopsis WRKY proteins specifically recognized NPR1 promoter W-box sequences.
- Taxon
- Arabidopsis
Arabidopsise0 - Gene
- NPR1e0
- Outcome
- recognized specifically by SA-induced WRKY DNA binding proteinse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“W-box sequences in the promoter region of the NPR1 gene that are recognized specifically by SA-induced WRKY DNA binding proteins from Arabidopsis”
Claim · model-written summary
W-box mutations abolished recognition by WRKY proteins.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- Mutations in these W-box sequencese0
- Outcome
- recognition by WRKY DNA binding proteinse0
- Direction
- abolishede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mutations in these W-box sequences abolished their recognition by WRKY DNA binding proteins”
e1“from Arabidopsis”
Claim · model-written summary
W-box mutations prevented promoter activation of a downstream reporter gene.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- Mutations in these W-box sequencese0
- Outcome
- activate a downstream reporter genee0
- Direction
- unablee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mutations in these W-box sequences abolished their recognition by WRKY DNA binding proteins, rendered the promoter unable to activate a downstream reporter gene”
e1“from Arabidopsis”
Claim · model-written summary
W-box mutations compromised NPR1 complementation for SA-induced defense gene expression and disease resistance.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- Mutations in these W-box sequencese0
- Outcome
- ability of NPR1 to complement npr1 mutants for SA-induced defense gene expression and disease resistancee0
- Direction
- compromisede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mutations in these W-box sequences abolished their recognition by WRKY DNA binding proteins, rendered the promoter unable to activate a downstream reporter gene, and compromised the ability of NPR1 to complement npr1 mutants for SA-induced defense gene expression and disease resistance”
e1“from Arabidopsis”
Claim · model-written summary
The authors infer that WRKY genes positively regulate NPR1 upstream during defense activation.
- Taxon
- ArabidopsisArabidopsise1
- Gene
- WRKY genese0
- Outcome
- NPR1e0
- Direction
- positively regulatee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“certain WRKY genes act upstream of NPR1 and positively regulate its expression during the activation of plant defense responses”
e1“from Arabidopsis”
Claim · model-written summary
SA-induced expression of several WRKY genes was independent of NPR1.
- Taxon
- ArabidopsisArabidopsise1
- Gene
- WRKY genese0
- Outcome
- SA-induced expressione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SA-induced expression of a number of WRKY genes was independent of NPR1”
e1“from Arabidopsis”
The same questions in other papers
loss of function · molecular: expression · ArabidopsisPositive and negative results
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- negative
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