Abstract
RNA stability is important in post-transcriptional gene expression. However, its role during plant male gametophyte development has not been studied in detail, probably because of technical difficulties and because earlier work suggested that, in general, mRNAs expressed during late pollen development would be stable. We determined mRNA stabilities of 12 pollen-expressed genes. We measured pollen mRNA decay during in planta development and in vitro maturation after transcriptional inhibition with ActinomycinD (ActD). Because five of these genes were also expressed in somatic cells we also measured RNA decay in BY2 cells after transcriptional inhibition with ActD. Ten of the mRNAs were highly stable during pollen development (t1/2 > 30 h), including all seven pollen-specific mRNAs. However, two mRNAs showed a logarithmic decrease as pollen development proceeded, both in planta and in vitro. In fact, for one of these two, GUT8-2b, the mRNA level decreased significantly within 3 h. Our results show that many pollen-expressed mRNAs are indeed long-lived but that at least some mRNAs undergo decay during pollen maturation. Thus, there is no overall cytoplasmic condition in pollen that stabilizes mRNAs and specific mRNA turnover mechanisms must exist. We detected reciprocal mRNA stabilities with two genes that are expressed in both BY2 cells and pollen. GUT15 mRNA, known to be unstable (t1/2 < 3 h) in BY2 cells, was highly stable in pollen. In contrast, GRP2 mRNA decayed in pollen but was highly stable in BY2 cells. Therefore, we conclude that mRNA stability can be tissue-specific.
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Claims 8
Claim · model-written summary
Ten of the mRNAs measured were highly stable during pollen development (t1/2 > 30 h).
Verbatim
e1“Ten of the mRNAs were highly stable during pollen development (t1/2 > 30 h), including all seven pollen-specific mRNAs”
e4“However, its role during plant male gametophyte development has not been studied in detail”
Claim · model-written summary
All seven pollen-specific mRNAs were highly stable during pollen development.
Verbatim
e1“Ten of the mRNAs were highly stable during pollen development (t1/2 > 30 h), including all seven pollen-specific mRNAs”
e4“However, its role during plant male gametophyte development has not been studied in detail”
Claim · model-written summary
Two mRNAs showed a logarithmic decrease as pollen development proceeded, both in planta and in vitro.
Verbatim
e2“two mRNAs showed a logarithmic decrease as pollen development proceeded, both in planta and in vitro”
e4“However, its role during plant male gametophyte development has not been studied in detail”
Claim · model-written summary
The GUT8-2b mRNA level decreased significantly within 3 h during pollen maturation.
Verbatim
e3“In fact, for one of these two, GUT8-2b, the mRNA level decreased significantly within 3 h”
e4“However, its role during plant male gametophyte development has not been studied in detail”
Claim · model-written summary
Specific mRNA turnover mechanisms must exist in pollen because no overall cytoplasmic condition stabilizes mRNAs there.
Verbatim
e5“Thus, there is no overall cytoplasmic condition in pollen that stabilizes mRNAs and specific mRNA turnover mechanisms must exist”
e4“However, its role during plant male gametophyte development has not been studied in detail”
Claim · model-written summary
GUT15 mRNA was highly stable in pollen, in contrast to BY2 cells.
Verbatim
e6“GUT15 mRNA, known to be unstable (t1/2 < 3 h) in BY2 cells, was highly stable in pollen”
e4“However, its role during plant male gametophyte development has not been studied in detail”
Claim · model-written summary
GRP2 mRNA decayed in pollen.
Verbatim
e7“GRP2 mRNA decayed in pollen but was highly stable in BY2 cells”
e4“However, its role during plant male gametophyte development has not been studied in detail”
Claim · model-written summary
GRP2 mRNA was highly stable in BY2 cells.
Verbatim
e7“GRP2 mRNA decayed in pollen but was highly stable in BY2 cells”