Abstract
The present work is the first report on in vitro regeneration of an elite date palm cultivar, Deglet Bey (Mnakher) through both somatic embryogenesis and direct shootformation from young leaf explants cultured on MS agar-solidified medium supplemented with 10 mg.l−1 2,4-dichlorophenoxyacetic acidfor 8 months. Factors affecting embryogenic callus and shoot initiation, including antioxidants, adsorbents and 2,4-D concentrations, were investigated. Embryogenic suspensions and culture of shoots in temporary immersion bioreactor (TIB) were developed to improve differentiation of embryogenic callus and proliferation of regenerated shoots, respectively. The yield of cotyledonary somatic embryos produced in half-strength MS liquid media, especially when enriched with 2 mg.l−1 2,4-dichlorophenoxyacetic acid, were shown to be greater than in agar-solidified medium, namely 501 versus 29 per 0.5 g fresh weight of embryogenic callus. The culture of shoot clusters in TIB with an immersion frequency of 5 min every 8 h with MS liquid medium containing 0.04 mg.l−1 α-naphthalenacetic acid, 0.2 mg.l−1 6-benzylaminopurine 0.02 mg.l−1 kinetin for 6 weeks has clearly improved the yield of regenerated shoots per 15 g of shoot clusters. Indeed, it increased 5.5-fold in comparison with that regenerated on agar-solidified medium. For development into plantlets, cotyledonary somatic embryos and regenerated shoots were cultured on MS agar-solidified medium free of 2,4-D and MS medium comprising 0.1 mg.l−1 NAA, respectively.
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Claims 2
Claim · model-written summary
Half-strength liquid medium enriched with 2,4-D yielded more cotyledonary embryos than agar medium, 501 versus 29.
- Taxon
- date palm
Phoenix dactyliferat0 - Treatment
- half-strength MS liquid media, especially when enriched with 2 mg.l−1 2,4-dichlorophenoxyacetic acide0
- Outcome
- yield of cotyledonary somatic embryose0
- Direction
- greatere0
- Value
- 501 versus 29 per 0.5 g fresh weight of embryogenic calluse0
- Comparator
- agar-solidified mediume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The yield of cotyledonary somatic embryos produced in half-strength MS liquid media, especially when enriched with 2 mg.l−1 2,4-dichlorophenoxyacetic acid, were shown to be greater than in agar-solidified medium, namely 501 versus 29 per 0.5 g fresh weight of embryogenic callus”
t0“Date Palm”
Claim · model-written summary
Temporary immersion increased regenerated shoot yield 5.5-fold compared with agar medium.
- Taxon
- date palmPhoenix dactyliferat0
- Treatment
- TIBe0
- Outcome
- yield of regenerated shoots per 15 g of shoot clusterse0
- Direction
- increasede0
- Value
- 5.5-folde0
- Comparator
- agar-solidified mediume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The culture of shoot clusters in TIB with an immersion frequency of 5 min every 8 h with MS liquid medium containing 0.04 mg.l−1 α-naphthalenacetic acid, 0.2 mg.l−1 6-benzylaminopurine 0.02 mg.l−1 kinetin for 6 weeks has clearly improved the yield of regenerated shoots per 15 g of shoot clusters. Indeed, it increased 5.5-fold in comparison with that regenerated on agar-solidified medium”
t0“Date Palm”
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.