Abstract
Previous QTL mapping identified a Brassica nigra homolog to Arabidopsis thaliana CO as a candidate gene affecting flowering time in B. nigra. Transformation of an A. thaliana co mutant with two different alleles of the B. nigra CO (Bni COa) homolog, one from an early-flowering B. nigra plant and one from a late one, did not show any differential effect of the two alleles on flowering time. The DNA sequence of the coding region of the two alleles was also identical, showing that nucleotide variation influencing flowering time must reside outside the coding region of Bni COa. In contrast, the nucleotide sequence of the B. nigra COL1 (Bni COL1) gene located 3.5 kb upstream of Bni COa was highly diverged between the alleles from early and late plants. One indel polymorphism in the Bni COL1 coding region, present in several natural populations of B. nigra, displayed a significant association with flowering time within a majority of these populations. These data indicate that a quantitative trait nucleotide (QTN) affecting flowering time is located within or close to the Bni COL1 gene. The intergenic sequence between Bni COL1 and Bni COa displayed a prominent peak of divergence 1 kb downstream of the Bni COL1 coding region. This region could contain regulatory elements for the downstream Bni COa gene. Our data suggest that a naturally occurring QTN for flowering time affects the function or expression of either Bni COL1 or Bni COa.
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Claims 6
Claim · model-written summary
Two Bni COa alleles did not differentially affect flowering time in the Arabidopsis co mutant.
- Taxon
- A. thaliana
Arabidopsis thalianae0 - Material
- co mutante0
- Treatment
- two different allelese0
- Gene
- Bni COae0
- Outcome
- flowering timee0
- Direction
- did not show any differential effecte0
- Comparator
- two allelese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transformation of an A. thaliana co mutant with two different alleles of the B. nigra CO (Bni COa) homolog, one from an early-flowering B. nigra plant and one from a late one, did not show any differential effect of the two alleles on flowering time”
Claim · model-written summary
The two Bni COa coding sequences were identical.
- Taxon
- Brassica nigra
_leaves.JPG?width=60)
Brassica nigrat0 - Gene
- Bni COae1
- Outcome
- DNA sequence of the coding regione0
- Direction
- identicale0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_leaves.JPG?width=500)
Verbatim
e0“The DNA sequence of the coding region of the two alleles was also identical”
t0“Brassica nigra”
e1“B. nigra CO (Bni COa) homolog”
Claim · model-written summary
Bni COL1 sequence was highly diverged between early and late alleles.
- Taxon
- B. nigraBrassica nigrae0
- Gene
- Bni COL1e0
- Outcome
- nucleotide sequencee0
- Direction
- highly divergede0
- Comparator
- alleles from early and late plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the nucleotide sequence of the B. nigra COL1 (Bni COL1) gene located 3.5 kb upstream of Bni COa was highly diverged between the alleles from early and late plants”
Claim · model-written summary
A Bni COL1 coding indel was significantly associated with flowering time in most populations.
- Taxon
- B. nigraBrassica nigrae0
- Gene
- Bni COL1e0
- Outcome
- flowering timee0
- Qualifier
- majority of these populationse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“One indel polymorphism in the Bni COL1 coding region, present in several natural populations of B. nigra, displayed a significant association with flowering time within a majority of these populations”
Claim · model-written summary
The intergenic region showed a divergence peak downstream of Bni COL1.
- Taxon
- Brassica nigraBrassica nigrat0
- Gene
- Bni COL1e0
- Outcome
- peak of divergencee0
- Value
- 1 kbe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The intergenic sequence between Bni COL1 and Bni COa displayed a prominent peak of divergence 1 kb downstream of the Bni COL1 coding region”
t0“Brassica nigra”
Claim · model-written summary
A flowering-time QTN was inferred within or near Bni COL1.
- Taxon
- Brassica nigraBrassica nigrat0
- Gene
- Bni COL1e0
- Outcome
- flowering timee0
- Qualifier
- within or close to the Bni COL1 genee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a quantitative trait nucleotide (QTN) affecting flowering time is located within or close to the Bni COL1 gene”
t0“Brassica nigra”