Abstract
The hexaploid wheat 'Prelude' (2n = 42 = AABBDD) contains the endosperm protein electrophoretic band 40, but not band 45, while the extracted tetraploid 'Tetraprelude' (2n = 28 = AABB) contains electrophoretic band 45 but not band 40. Moreover, F 3 progeny from the cross between backcross lines of 'Prelude' and 'Marquis' segregated as if both of these proteins were controlled by alleles of one gene, or by two very closely linked genes. Examination of the F 3 seed from the cross 'Prelude' × 'Canthatch' 7D nullisomic showed that band 45 was not expressed in any of the 334 F 2 families, including nine nullisomics. Together, these results indicate that band 45 endosperm protein is not suppressed by a gene on chromosome 7D. Furthermore, removal of the D genome from 'Prelude' resulted in increased kernel hardness and increased gluten elastic strength but a decrease in sodium dodecyl sulfate sedimentation volume. However, the sodium dodecyl sulfate sedimentation volume of 'Tetraprelude', was the highest among the tetraploid cultivars evaluated.Key words: wheat, sodium dodecyl sulfate electrophoresis, endosperm protein, gene expression.
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Claims 9
Claim · model-written summary
Prelude has endosperm protein band 40 but lacks band 45.
- Taxon
- wheat
Triticum aestivume0 - Material
- Preludee0
- Outcome
- endosperm protein electrophoretic band 40, but not band 45e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The hexaploid wheat 'Prelude' (2n = 42 = AABBDD) contains the endosperm protein electrophoretic band 40, but not band 45”
Claim · model-written summary
Tetraprelude has band 45 but lacks band 40.
- Taxon
- wheatTriticum aestivumt0
- Material
- Tetrapreludee0
- Outcome
- electrophoretic band 45 but not band 40e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the extracted tetraploid 'Tetraprelude' (2n = 28 = AABB) contains electrophoretic band 45 but not band 40”
t0“wheat”
Claim · model-written summary
Segregation of the two proteins was consistent with alleles of one gene or two closely linked genes.
- Taxon
- wheatTriticum aestivumt0
- Material
- 'Prelude' and 'Marquis'e0
- Outcome
- both of these proteins were controlled by alleles of one gene, or by two very closely linked genese0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“F 3 progeny from the cross between backcross lines of 'Prelude' and 'Marquis' segregated as if both of these proteins were controlled by alleles of one gene, or by two very closely linked genes”
t0“wheat”
Claim · model-written summary
Band 45 was absent in all 334 F2 families, including nine nullisomics.
- Taxon
- wheatTriticum aestivumt0
- Outcome
- band 45 was not expressede0
- Value
- 334 F 2 familiese0
- Qualifier
- including nine nullisomicse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“band 45 was not expressed in any of the 334 F 2 families, including nine nullisomics”
t0“wheat”
Claim · model-written summary
The authors infer that chromosome 7D does not suppress band 45.
- Taxon
- wheatTriticum aestivumt0
- Gene
- gene on chromosome 7De0
- Outcome
- band 45 endosperm protein is not suppressede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“band 45 endosperm protein is not suppressed by a gene on chromosome 7D”
t0“wheat”
Claim · model-written summary
Removing the D genome increased kernel hardness.
- Taxon
- wheatTriticum aestivumt0
- Material
- Preludee0
- Treatment
- removal of the D genomee0
- Outcome
- kernel hardnesse0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“removal of the D genome from 'Prelude' resulted in increased kernel hardness”
t0“wheat”
Claim · model-written summary
Removing the D genome increased gluten elastic strength.
- Taxon
- wheatTriticum aestivumt0
- Material
- Preludee0
- Treatment
- removal of the D genomee0
- Outcome
- gluten elastic strengthe0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“removal of the D genome from 'Prelude' resulted in increased kernel hardness and increased gluten elastic strength”
t0“wheat”
Claim · model-written summary
Removing the D genome decreased sodium dodecyl sulfate sedimentation volume.
- Taxon
- wheatTriticum aestivumt0
- Material
- Preludee0
- Treatment
- removal of the D genomee0
- Outcome
- sodium dodecyl sulfate sedimentation volumee0
- Direction
- decreasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“removal of the D genome from 'Prelude' resulted in increased kernel hardness and increased gluten elastic strength but a decrease in sodium dodecyl sulfate sedimentation volume”
t0“wheat”
Claim · model-written summary
Tetraprelude had the highest sodium dodecyl sulfate sedimentation volume among the tetraploid cultivars evaluated.
- Taxon
- wheatTriticum aestivumt0
- Material
- Tetrapreludee0
- Outcome
- sodium dodecyl sulfate sedimentation volumee0
- Direction
- higheste0
- Comparator
- tetraploid cultivars evaluatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“sodium dodecyl sulfate sedimentation volume of 'Tetraprelude', was the highest among the tetraploid cultivars evaluated”
t0“wheat”